| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB9167-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4517-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61658-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4517-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61657-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50529-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64392-0.025mg | |||
![Neutrophil Elastase/ELA2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Neutrophil_Elastase_DY451705_ELISA_2461.jpg "Neutrophil Elastase/ELA2 [Biotin]")
![Western Blot: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167] - Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - Analysis of human bone marrow tissue lysate with 0.5 ug/ml of hELA-2 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Antibody. A specific band was detected for hELA-2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(950334)-Western-Blot-MAB9167-img0001.jpg "Western Blot: Neutrophil Elastase/ELA2 Antibody (950334) - Azide and BSA Free [MAB9167] - Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - Analysis of human bone marrow tissue lysate with 0.5 ug/ml of hELA-2 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Antibody. A specific band was detected for hELA-2 at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.")
![Immunocytochemistry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free [MAB9167] - Neutrophil Elastase/ELA2 was detected in immersion fixed THP-1 cell using Neutrophil Elastase/ELA2 Monoclonal Antibody at 8 ug/ml dilution. Cells were stained using Donkey anti-Mouse IgG Secondary Antibody [NL557] (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasmic.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(950334)---Azide-Free-Immunocytochemistry-MAB9167-img0006.jpg)
![Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (950334) [MAB9167] - ELA-2 was detected in immersion fixed paraffin-embedded sections of human lymphoma using ELA-2 Monoclonal Antibody at 5 ug/ml. Tissue was stained using Mouse IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neutrophils .](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(950334)-Immunohistochemistry-MAB9167-img0002.jpg)
![<b>Biological Strategies Validation. </b>Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (950334) - Azide Free [MAB9167] - THP-1 human acute monocytic leukemia cell line treated with 3 uM monensin for 3 hours was stained with Mouse Anti-Human Neutrophil Elastase/ELA2 Monoclonal Antibody (Catalog # MAB9167, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(950334)---Azide-Free-Flow-Cytometry-MAB9167-img0005.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(6B6B10)-Western-Blot-NBP2-61658-img0005.jpg "Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis of paraffin-embedded stomach cancer tissues using ELANE mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(6B6B10)-Immunohistochemistry-NBP2-61658-img0003.jpg)
![Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis of Hela cells using ELANE mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(6B6B10)-Flow-Cytometry-NBP2-61658-img0002.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mAb against human ELANE (AA: 140-267) recombinant protein. (Expected MW is 39 kDa)](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(6B6B10)-Western-Blot-NBP2-61658-img0004.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Analysis using ELANE mouse mAb against U937 (1), SPC-A-1 (2), and COS7 (3) cell lysate.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(6B6B10)-Western-Blot-NBP2-61658-img0006.jpg)
![ELISA: Neutrophil Elastase/ELA2 Antibody (6B6B10) [NBP2-61658] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(6B6B10)-ELISA-NBP2-61658-img0001.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (8X4E6) [NBP3-16724] - Analysis of extracts of various cell lines, using Neutrophil Elastase/ELA2 antibody (NBP3-16724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(8X4E6)-Western-Blot-NBP3-16724-img0003.jpg "Western Blot: Neutrophil Elastase/ELA2 Antibody (8X4E6) [NBP3-16724] - Analysis of extracts of various cell lines, using Neutrophil Elastase/ELA2 antibody (NBP3-16724) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunohistochemistry: Neutrophil Elastase/ELA2 Antibody (8X4E6) [NBP3-16724] - Analysis of human spleen using Neutrophil Elastase/ELA2 Rabbit mAb (NBP3-16724) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(8X4E6)-Immunohistochemistry-NBP3-16724-img0002.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody (8X4E6) [NBP3-16724] - Mouse bone marrow using Neutrophil Elastase/ELA2 antibody (NBP3-16724) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(8X4E6)-Immunohistochemistry-Paraffin-NBP3-16724-img0001.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(6B6G6)-Western-Blot-NBP2-61657-img0004.jpg "Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis using ELANE mAb against HEK293 (1) and ELANE (AA: 140-267)-hIgGFc transfected HEK293 (2) cell lysate.")
![Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis of K562 cells using ELANE mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(6B6G6)-Flow-Cytometry-NBP2-61657-img0002.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis using ELANE mAb against human ELANE (AA: 140-267) recombinant protein. (Expected MW is 39 kDa)](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(6B6G6)-Western-Blot-NBP2-61657-img0003.jpg)
![Western Blot: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Analysis using ELANE mouse mAb against HL-60 (1), THP-1 (2), MOLT4 (3), C6 (4), and K562 (5) cell lysate.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(6B6G6)-Western-Blot-NBP2-61657-img0005.jpg)
![ELISA: Neutrophil Elastase/ELA2 Antibody (6B6G6) [NBP2-61657] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(6B6G6)-ELISA-NBP2-61657-img0001.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody [NBP2-57576] - Staining in human bone marrow and cerebral cortex tissues using anti-ELANE antibody. Corresponding ELANE RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-Immunohistochemistry-Paraffin-NBP2-57576-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody [NBP2-57576] - Staining in human bone marrow and cerebral cortex tissues using anti-ELANE antibody. Corresponding ELANE RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody [NBP2-57576] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-Immunohistochemistry-Paraffin-NBP2-57576-img0003.jpg)
![Immunohistochemistry-Paraffin: Neutrophil Elastase/ELA2 Antibody [NBP2-57576] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-Immunohistochemistry-Paraffin-NBP2-57576-img0002.jpg)
![Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) [NB100-64392] - Leukocytes were isolated from BAL fluid of a CF patient and stained for analysis by flow cytometry. Macrophages were found to have scavenged substantial neutrophil elastase from the airways while minimal was observed on the surface of T cells from the same patient as expected. This antibody works well for staining multiple cell types and obtains reliable fluorescence in the FITC channel. When used for single-stained beads compensation controls, consistent signal is obtained provided that the cytometer is accurately calibrated. One thing to be aware of is that different lots of this antibody have slightly different concentrations. Flow cytometry image submitted by a verified customer review using the Alexa Fluor 488 conjugated format of this antibody.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-Antibody-(39A)-Flow-Cytometry-NB100-64392-img0001.jpg "Flow Cytometry: Neutrophil Elastase/ELA2 Antibody (39A) [NB100-64392] - Leukocytes were isolated from BAL fluid of a CF patient and stained for analysis by flow cytometry. Macrophages were found to have scavenged substantial neutrophil elastase from the airways while minimal was observed on the surface of T cells from the same patient as expected. This antibody works well for staining multiple cell types and obtains reliable fluorescence in the FITC channel. When used for single-stained beads compensation controls, consistent signal is obtained provided that the cytometer is accurately calibrated. One thing to be aware of is that different lots of this antibody have slightly different concentrations. Flow cytometry image submitted by a verified customer review using the Alexa Fluor 488 conjugated format of this antibody.")
![ELISA: Neutrophil Elastase/ELA2 ELISA Kit [NBP1-91266] - Standard curve.](http://images.novusbio.com/fullsize2/Neutrophil-Elastase-ELA2-ELISA-Kit-ELISA-NBP1-91266-img0001.jpg "ELISA: Neutrophil Elastase/ELA2 ELISA Kit [NBP1-91266] - Standard curve.")