| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26329-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26330-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86704-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| HRP | NBP2-79597-5Plates | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB52351 | |||
| Carrier Free | MAB52351-SP | |||
| Carrier Free | FAB52351V-100UG | |||
| Carrier Free | FAB52351U-100UG | |||
| Carrier Free | FAB52351T-100UG | |||
| Carrier Free | FAB52351S-100UG | |||
| Carrier Free | FAB52351R-100UG | |||
![ELISA: Semaphorin 4D/CD100 Antibody (5D12) [NBP3-26329] - The Binding Activity of Human Semaphorin 4D/CD100 with Semaphorin 4D/CD100 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Semaphorin 4D/CD100 at 2 ug/mL can bind Semaphorin 4D/CD100 Antibody, the EC50 is 60.38-152.6 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26329_human-semaphorin-4d-cd100-mab-5d12-262202412272311.jpg "ELISA: Semaphorin 4D/CD100 Antibody (5D12) [NBP3-26329] - The Binding Activity of Human Semaphorin 4D/CD100 with Semaphorin 4D/CD100 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Semaphorin 4D/CD100 at 2 ug/mL can bind Semaphorin 4D/CD100 Antibody, the EC50 is 60.38-152.6 ng/mL.")
![ELISA: Semaphorin 4D/CD100 Antibody (5D12) [NBP3-26329] - The Binding Activity of Mouse Semaphorin 4D/CD100 with Semaphorin 4D/CD100 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Semaphorin 4D/CD100 Antibody at 2 ug/mL can bind Mouse Semaphorin 4D/CD100, the EC50 is 5.829-16.86 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26329_human-semaphorin-4d-cd100-mab-5d12-26220241227238.jpg)
![Flow Cytometry: Semaphorin 4D/CD100 Antibody (5D12) [NBP3-26329] - Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2ug/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.](http://images.novusbio.com/fullsize/nbp3-26329_human-semaphorin-4d-cd100-mab-5d12-282202414183620.jpg)
![ELISA: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] - The Binding Activity of Mouse Semaphorin 4D/CD100 with Semaphorin 4D/CD100 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Semaphorin 4D/CD100 at 2 ug/mL can bind Semaphorin 4D/CD100 Antibody, the EC50 is 98.14-174.8 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26330_human-semaphorin-4d-cd100-mab-21e8-262202412272331.jpg "ELISA: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] - The Binding Activity of Mouse Semaphorin 4D/CD100 with Semaphorin 4D/CD100 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Semaphorin 4D/CD100 at 2 ug/mL can bind Semaphorin 4D/CD100 Antibody, the EC50 is 98.14-174.8 ng/mL.")
![ELISA: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] - The Binding Activity of Human Semaphorin 4D/CD100 with Semaphorin 4D/CD100 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Semaphorin 4D/CD100 at 2 ug/mL can bind Semaphorin 4D/CD100 Antibody, the EC50 is 1.082-4.805 ng/mL.](http://images.novusbio.com/fullsize/nbp3-26330_human-semaphorin-4d-cd100-mab-21e8-262202412272340.jpg)
![Flow Cytometry: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] - Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2ug/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.](http://images.novusbio.com/fullsize/nbp3-26330_human-semaphorin-4d-cd100-mab-21e8-282202414312745.jpg)
![Immunohistochemistry: Semaphorin 4D/CD100 Antibody [NBP1-86704] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Semaphorin-4D-CD100-Antibody-Immunohistochemistry-NBP1-86704-img0003.jpg "Immunohistochemistry: Semaphorin 4D/CD100 Antibody [NBP1-86704] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.")
![ELISA: Mouse Semaphorin 4D/CD100 ELISA Kit (Colorimetric) [NBP2-75126] - Samples were spiked with high concentrations of Mouse Semaphorin 4D/CD100 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75126_mouse-semaphorin-4d-cd100-elisa-kit-colorimetric-132202416254036.jpg "ELISA: Mouse Semaphorin 4D/CD100 ELISA Kit (Colorimetric) [NBP2-75126] - Samples were spiked with high concentrations of Mouse Semaphorin 4D/CD100 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Semaphorin 4D/CD100 ELISA Kit (Colorimetric) [NBP2-75126] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75126_mouse-semaphorin-4d-cd100-elisa-kit-colorimetric-432024915858.jpg)
![Western Blot: SEMA4D Overexpression Lysate (Adult Normal) [NBL1-15803] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SEMA4D.](http://images.novusbio.com/fullsize/SEMA4D-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15803-img0002.jpg "Western Blot: SEMA4D Overexpression Lysate (Adult Normal) [NBL1-15803] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SEMA4D.")
![Western Blot: Semaphorin 4D/CD100 Antibody (JE63-21) [NBP3-32119] - Western blot analysis of Semaphorin 4D/CD100 on different lysates with Rabbit anti-Semaphorin 4D/CD100 antibody (NBP3-32119) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: Daudi cell lysate Lane 3: HL-60 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 96 kDa Observed band size: 130 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32119) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32119_rabbit-semaphorin-4d-cd100-mab-je63-21-278202415242698.jpg "Western Blot: Semaphorin 4D/CD100 Antibody (JE63-21) [NBP3-32119] - Western blot analysis of Semaphorin 4D/CD100 on different lysates with Rabbit anti-Semaphorin 4D/CD100 antibody (NBP3-32119) at 1/500 dilution. Lane 1: Jurkat cell lysate Lane 2: Daudi cell lysate Lane 3: HL-60 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 96 kDa Observed band size: 130 kDa Exposure time: 30 seconds; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32119) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Sandwich ELISA: Semaphorin 4D/CD100 Antibody Pair [HRP] [NBP2-79597] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize/Semaphorin-4D-CD100-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79597-img0001.jpg "Sandwich ELISA: Semaphorin 4D/CD100 Antibody Pair [HRP] [NBP2-79597] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
![Flow Cytometry: Semaphorin 4D/CD100 Antibody (010) [NBP2-90666] - Flow cytometric analysis of SEMA4D(CD100) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse SEMA4D(CD100), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Semaphorin-4D-CD100-Antibody-010-Flow-Cytometry-NBP2-90666-img0002.jpg "Flow Cytometry: Semaphorin 4D/CD100 Antibody (010) [NBP2-90666] - Flow cytometric analysis of SEMA4D(CD100) expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse SEMA4D(CD100), then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")