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![Western Blot: PLA2R1 Antibody (12-6-5) [NBP2-50248] - 1ug of recombinant full length extracellular domain of human PLA2R (NC8) and a N-terminus fragment of PLA2R (NC3) were subjected to SDS PAGE followed by western blot using Ms anti-PLAR (Cl12-6-5) at dilution 1:10000.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Western-Blot-NBP2-50248-img0001.jpg "Western Blot: PLA2R1 Antibody (12-6-5) [NBP2-50248] - 1ug of recombinant full length extracellular domain of human PLA2R (NC8) and a N-terminus fragment of PLA2R (NC3) were subjected to SDS PAGE followed by western blot using Ms anti-PLAR (Cl12-6-5) at dilution 1:10000.")
![Immunocytochemistry/Immunofluorescence: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Staining of PLA2R over-expressing podocytes revealing cell surface expression of the PLA2R receptor. Dilution 1:400 (under 40x lens).](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Immunocytochemistry-Immunofluorescence-NBP2-50248-img0002.jpg)
![Immunohistochemistry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Membranous nephropathy kidney showing staining of PLA2R in the capillary wall of the glomerular basement membrane. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection system.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Immunohistochemistry-NBP2-50248-img0006.jpg)
![Flow Cytometry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - A surface stain was performed on HeLa cells with PLA2R1 Antibody (12-6-5) NBP2-50248PE (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 2.5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Flow-Cytometry-NBP2-50248-img0007.jpg)
![Immunohistochemistry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Normal human kidney showing glomerular specific staining. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection system.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Immunohistochemistry-NBP2-50248-img0004.jpg)
![Immunohistochemistry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Normal human kidney showing podocyte specific staining. IHC Protocol Info: HIER pH8 (CC1) for 64 minutes, 36 minute primary antibody (PLA2R Mouse 12-6-5) incubation at room temperature using a dilution of 1:5000, using Ventana Ultraview DAB detection system.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Immunohistochemistry-NBP2-50248-img0005.jpg)
![Flow Cytometry: PLA2R1 Antibody (12-6-5) [NBP2-50248] - Overlay flow cytometry histograms of wild type (dark blue line) and over-expressing PLA2R podocytes (low expressor, green line; high expressor, red line) stained with Ms Cl12-6-5) at dilution 1:200. Negative controls were unlabelled cells (turquoise line) and mouse IgG (grey line).](http://images.novusbio.com/fullsize/PLA2R1-Antibody-12-6-5-Flow-Cytometry-NBP2-50248-img0003.jpg)
![Flow Cytometry: PLA2R1 Antibody [NBP1-46116] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PLA2R1-Antibody-Flow-Cytometry-NBP1-46116-img0001.jpg "Flow Cytometry: PLA2R1 Antibody [NBP1-46116] - Analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.")