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28 Full Site Results for "lysates": Protocol
Immunoprecipitation Protocol (NB200-174)
Immunoprecipitation 1. Total cell lysates (0.25 mg) from NIH3T3 cells which have deleted the Arf gene and from NIH3T3 cells expressing an HA-tagged version of p19Arf were incubated with control rat IgG or anti-p19Arf (NB 200-174) and protein G- Seph
Western Blot protocol for Bestrophin 1 Antibody (NB300-164)
Procedure Guide for NB 300-164 Monoclonal Anti-Bestrophin Western Blot Procedure 1. Run cell lysates** on an SDS-PAGE gel. 2. Transfer the proteins to PVDF. 3. Block the membrane in 1% Carnation instant milk in PBS + 0.1% Tween 20 (with 0.1mM CaCl2
Western Blot Protocol Specific for Survivin Antibody (NB500-201)
Western Blot Procedure 1) Cells were pelleted, washed in 1XPBS, suspended in ice water (~ 5 x 10(6) cells/ml), and placed on ice 2) Lysates were prepared with the addition of 2X lysis buffer [2% SDS/ 50mM Tris-HCl / 10% glycerol] 3) Lysates were heat
Western Blot Protocol Specific for Survivin Antibody (NB500-201)
Western Blot Procedure 1) Cells were pelleted, washed in 1XPBS, suspended in ice water (~ 5 x 10(6) cells/ml), and placed on ice 2) Lysates were prepared with the addition of 2X lysis buffer [2% SDS/ 50mM Tris-HCl / 10% glycerol] 3) Lysates were heat
Western Blot protocol for Bestrophin 1 Antibody (NB300-164)
Procedure Guide for NB 300-164 Monoclonal Anti-Bestrophin Western Blot Procedure 1. Run cell lysates** on an SDS-PAGE gel. 2. Transfer the proteins to PVDF. 3. Block the membrane in 1% Carnation instant milk in PBS + 0.1% Tween 20 (with 0.1mM CaCl2
Western Blot Protocols specific for 15-PGDH Antibody (NB200-179)
Western Blot I (LoVo lysates) 1. Perform SDS-PAGE (4-12%) on samples to be analyzed, loading 20ug of total protein per lane. 2. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatu
Western Blot Protocol for PPM1B Antibody (NBP1-04335)
Western Blot: 1. Sample preparation: SDS loading buffer was added to MCF7 cell lysates and recombinant PPM1B protein. The solution was boiled before loading on a 15% SDS-PAGE 2. Blocking: 5% skim milk in PBS-Tween, for 1hr at room temperature, 3 tim
Protocol specific for VAV3 Antibody (NB300-817)
Lysis: Cell pellets were washed with ice-cold PBS. 1 ml of RIPA buffer was added per 1E8 cells and incubated on ice for 20 min, vortexing 2-3 times, briefly. The lysate was aliquotted into 1.5 ml microfuge tubes and centrifuged at 13,000 rpm for 5 m
Protocol specific for VAV3 Antibody (NB300-817)
Lysis: Cell pellets were washed with ice-cold PBS. 1 ml of RIPA buffer was added per 1E8 cells and incubated on ice for 20 min, vortexing 2-3 times, briefly. The lysate was aliquotted into 1.5 ml microfuge tubes and centrifuged at 13,000 rpm for 5 m
Western Blot protocol for HIF-2 alpha/EPAS1 Antibody (NB100-122)
Western Blot Protocol Specific for HIF-2alpha Antibody (NB100-122) General considerations for Western blot analysis of HIF-alpha proteins 1. HIF-2alpha is degraded under normoxic conditions and it is stabilized at O2 concentrations below 5% or with
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