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Immunohistochemistry-Paraffin protocol for Pyruvate Carboxylase Antibody (NBP1-49536)
Pyruvate Carboxylase Antibody: https://www.novusbio.com/products/pyruvate-carboxylase-antibody_nbp1-49536 Immunohistochemistry-paraffin embedded sections Antigen Unmasking: Bring slides to a boil in 10 mM sodium citrate buffer pH 6.0 then maintain a
Antibody Competition protocol for SESN3 Protein (NBP1-44985PEP)
Antibody Competition Protocol for SESN3 Protein (NBP1-44985PEP): https://www.novusbio.com/products/sesn3-protein_nbp1-44985pep In this assay the antigen binding sites (Fab) of a particular antibody is allowed to bind to homologous antigenic peptide i
Western Blot protocol for USP9x Antibody (NBP1-48321)
USP9x Antibody: https://www.novusbio.com/products/usp9x-antibody_nbp1-48321 Western Blot Protocol 1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 40 ug of total protein per lane. 2. Transfer proteins to Nitrocellulose according to
Protocol specific for RCAN1 Antibody (NBP1-46852)
RCAN1 Antibody: https://www.novusbio.com/products/rcan1-antibody_nbp1-46853 Western Blot Protocol 1. Perform SDS-PAGE (4-12% MOPS) on samples to be analyzed, loading 40 ug of total protein per lane. 2. Transfer proteins to Nitrocellulose according to
Immunocytochemistry/Immunofluorescence Protocol for Lin28 Antibody (NBP1-49537)
LIN-28A Antibody: https://www.novusbio.com/products/lin-28a-antibody_nbp1-49537 Immunocytochemistry Protocol Culture cells to appropriate density in 35mm culture dishes or 6-well plates. 1. Pull off culture medium with and add 10% formalin to the
SDS-Page protocol for Sorbitol Dehydrogenase Recombinant Protein (NBP1-44460)
SDS-PAGE (NBP1-44460): https://www.novusbio.com/products/sorbitol-dehydrogenase-recombinant-protein_nbp1-44460 1. Prepare a 1.5 ml assay buffer. The final concentrations are 78 mM Triethanolamine, 183 mM D-Fructose, 0.2 mM beta-nicotinamide dinucleot
Western Blot protocol specific for Usp22 antibody (NBP1-49644)
Western Blot Protocol 1. Perform SDS-PAGE on samples to be analyzed, loading 40 ug of total protein per lane. 2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus. 3.
Antibody Competition protocol for SESN2 Protein (NBP1-44989PEP)
Antibody Competition Protocol for SESN2 Protein (NBP1-44989PEP): https://www.novusbio.com/products/sesn2-protein_nbp1-44989pep In this assay the antigen binding sites (Fab) of a particular antibody is allowed to bind to homologous antigenic peptide i
Chromatin Immunoprecipitation protocol for Histone H4 Antibody (NB21-2011)
Histone H4 [Monomethyl Arg3] Antibody: https://www.novusbio.com/products/histone-h4-antibody_nb21-2011 Chromatin Immunoprecipitation Protocol Cell Fixation and Preparation 1. Begin with a cell culture that has reached 80% confluency. 2. Add forma
Protocol specific for H3K36me2 antibody (NB21-1252)
Histone H3 [Dimethyl Lys36] Antibody: https://www.novusbio.com/products/histone-h3-antibody_nb21-1252 Western Blot Protocol 1. Perform SDS-PAGE on samples to be analyzed, loading 10 ug of histone preps per lane. 2. Transfer proteins to membrane ac
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