Lightning-Link Cy5 Products


Easy Cy5 labeling of your antibody with 30 seconds hands-on time. Cyanine dyes are commonly used as fluorescent labels for proteins, nucleic acids and small molecules. Cyanine Dye 5 (Cy5) has a strong absorption peak at 643nm and a secondary absorption peak at 605nm. The maximum emission peak is at 667nm, with an excitation coefficient of 2.5 x105 cm-1 M-1. Cy5 can be used as an alternative to Alexa Fluor 647, and the phycobiliprotein Allophycocyanin.

The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.

Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates.

As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.