CD31/PECAM-1 Antibody - BSA Free

Manufactured by Genomic Antibody Technology™. GAT FAQs

Cluster of differentiation (CD31), Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), is a 30kDa type I transmembrane glycoprotein expressed by endothelial cells, platelets, leukocytes and some lymphocytes (e.g., B and T-cell subsets). Structurally, PECAM-1 consists of six extracellular immunoglobulin-like domains which serve to support various functions including homophilic binding, maintaining high expression of PECAM-1 within endothelial intercellular junctions, and supporting endothelial cell migration (1,2). PECAM's homophilic binding underscores its role in vascular barrier integrity and leukocyte trans-endothelial movement. However, PECAM may also engage in heterophilic interactions with molecules such as glycosaminoglycans (GAG), integrin alpha 5 beta 3, CD38 in lymphocytes and CD177/PR3 in neutrophils (2).

PECAM's intracellular cytoplasmic domain consists of a sequence of 118 amino acids and contains serine and tyrosine (also referred to as immunoreceptor tyrosine-based inhibitory motifs-ITIMs) residues, which may be phosphorylated upon cellular stimulation (3). ITIMs are phosphorylated by Src-family kinases and non-Src family kinases (e.g., Csk), leading to a conformational change which supports interactions with Src homology 2 (SH2) domain containing proteins such as protein-tyrosine phosphatase, SHP-2 (1,2). Formation of SHP-2/PECAM-1 complexes induces endothelial cell migration through the dephosphorylation of focal adhesion kinase and regulation of RhoA activity (1). Signaling downstream of ITIM tyrosine phosphorylations also plays a role in PECAM's anti-apoptotic activity, a function which is independent of its interaction with SHP-2. In platelets and leukocytes, phosphorylation of PECAM's cytosolic domain is inhibitory, preventing their activation.

References

1. Lertkiatmongkol, P., Liao, D., Mei, H., Hu, Y., & Newman, P. J. (2016). Endothelial functions of PECAM-1 (CD31). Current Opinion in Hematology. https://doi.org/10.1097/MOH.0000000000000239.Endothelial

2. Privratsky, J. R., & Newman, P. J. (2014). PECAM-1: Regulator of endothelial junctional integrity. Cell and Tissue Research. https://doi.org/10.1007/s00441-013-1779-3

3. Newman, P. J., & Newman, D. K. (2003). Signal transduction pathways mediated by PECAM-1: New roles for an old molecule in platelet and vascular cell biology. Arteriosclerosis, Thrombosis, and Vascular Biology. https://doi.org/10.1161/01.ATV.0000071347.69358.D9

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P, Immunohistochemistry, In vivo assay, WB.

Showing Publications 1 - 10 of 17. Show All 17 Publications. Collapse Publications.

Publications using NBP1-71663	Applications	Species
Marycz K, Bourebaba N, Serwotka-Suszczak A et al. In Vitro Generated Equine Hepatic-Like Progenitor Cells as a Novel Potent Cell Pool for Equine Metabolic Syndrome (EMS) Treatment Stem Cell Reviews and Reports 2023-05-01 [PMID: 36658383] (Immunohistochemistry, Human)	Immunohistochemistry	Human
Smeda M, Jasztal A, Maleki EH et al. Endothelial-mesenchymal transition induced by metastatic 4T1 breast cancer cells in pulmonary endothelium in aged mice Frontiers in Molecular Biosciences 2022-11-24 [PMID: 36504711] (In vivo assay)	In vivo assay
Feng D, Zhou J, Liu H et al. Astrocytic NDRG2-PPM1A interaction exacerbates blood-brain barrier disruption after subarachnoid hemorrhage Science advances 2022-09-30 [PMID: 36179025] (ICC/IF, Mouse) Details: Dilution used in ICC/IF 1:100	ICC/IF	Mouse
Bae E, Huang P, Muller-Greven G et al. Integrin alpha 3 beta 1 promotes vessel formation of glioblastoma-associated endothelial cells through calcium-mediated macropinocytosis and lysosomal exocytosis Nature communications 2022-07-25 [PMID: 35879332] (ICC/IF, IHC-P, IHC-Fr, Human)	ICC/IF, IHC-P, IHC-Fr	Human
Zhang L, Soni S, Hekimoglu E et al. Impaired Autophagic Activity Contributes to the Pathogenesis of Bronchopulmonary Dysplasia: Evidence from Murine and Baboon Models Am. J. Respir. Cell Mol. Biol. 2020-05-06 [PMID: 32374619] (WB)	WB
Zhu NN, Lu MJ, Chen YQ et al. Autologous blood transfusion stimulates wound healing in diabetic mice through activation of the HIF-1 alpha pathway by improving the blood preservation solution FASEB J. 2020-03-23 [PMID: 32202355] (IHC-P, IF/IHC)	IHC-P, IF/IHC
Tsai HF, Toda-Peters K, Shen AQ. Glioblastoma adhesion in a quick-fit hybrid microdevice Biomed Microdevices 2019-03-21 [PMID: 30900024] (ICC/IF, Human)	ICC/IF	Human
Sadiq A, Menchetti I, Shah A et al. 5-HT1A Receptor Function Makes Wound Healing a Happier Process Front. Pharmacol. 2018-12-11 [PMID: 30618734] (IHC-P, Mouse)	IHC-P	Mouse
Smeda M, Kieronska A, et al. Nitric oxide deficiency and endothelial-mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice. Breast Cancer Res 2018-08-03 [PMID: 30075800] (WB, Mouse)	WB	Mouse
Smeda M, Kieronska A, et al. Dual antiplatelet therapy with clopidogrel and aspirin increases mortality in 4T1 metastatic breast cancer-bearing mice by inducing vascular mimicry in primary tumour. Oncotarget 2018-04-03 [PMID: 29707148] (WB, Mouse)	WB	Mouse
Ma Y, Huang YX, Chen YY. miRNA-34a-5p downregulation of VEGFA in endometrial stem cells contributes to the pathogenesis of endometriosis. Mol Med Rep. 2017-09-29 [PMID: 28990049] (Human) Details: This citation used the FITC form of this antibody.		Human
Abraham V, Parambath A, Joe DS, DeLisser HM. Influence of PECAM-1 ligand interactions on PECAM-1-dependent cell motility and filopodia extension. Physiol Rep. 2016-11-01 [PMID: 27895229] (Human, Mouse) Details: This citation used the DyLight 650 version of this antibody.		Human, Mouse
Svenson S, Case RI, Cole RO et al. Tumor Selective Silencing Using an RNAi-Conjugated Polymeric Nanopharmaceutical. Mol. Pharm. 2016-03-07 [PMID: 26835715] (IHC-P, Mouse)	IHC-P	Mouse
Woolf EC, Curley KL, Liu Q et al. The Ketogenic Diet Alters the Hypoxic Response and Affects Expression of Proteins Associated with Angiogenesis, Invasive Potential and Vascular Permeability in a Mouse Glioma Model PLoS ONE. 2015-06-18 [PMID: 26083629] (IHC-P, WB, Mouse)	IHC-P, WB	Mouse
Lee JH, Park KS, Alberobello AT et al. The Janus Kinases Inhibitor AZD1480 Attenuates Growth of Small Cell Lung Cancers In Vitro and In Vivo. Clin Cancer Res 2013-12-15 [PMID: 24158701] (IF/IHC, Human)	IF/IHC	Human
Pratheeshkumar P, Son YO, Budhraja A et al. Luteolin inhibits human prostate tumor growth by suppressing vascular endothelial growth factor receptor 2-mediated angiogenesis PLoS One 2012-01-01 [PMID: 23300633] (IF/IHC, Mouse)	IF/IHC	Mouse
Pratheeshkumar P, Budhraja A, Son YO et al. Quercetin Inhibits Angiogenesis Mediated Human Prostate Tumor Growth by Targeting VEGFR- 2 Regulated AKT/mTOR/P70S6K Signaling Pathways PLoS One 2012-01-01 [PMID: 23094058] (IF/IHC, Human)	IF/IHC	Human
Show All 17 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	WB	Mouse	09/05/2013	View Summary Application Western Blot Sample Tested Mouse glioma tissue and mouse normal brain tissue. Species Mouse Lot A
Enlarge	5 reviewed by: Verified Customer	IF	Mouse	09/05/2013	View Summary Application Immunofluorescence Sample Tested Mouse glioma tissue Species Mouse Lot A

Array NBP1-71663

• CD31/PECAM-1 Antibodies
• CD31/PECAM-1 Antibody Pair
• CD31/PECAM-1 ELISA Kits
• CD31/PECAM-1 Lysate
• CD31/PECAM-1 Protein
• CD31/PECAM-1 Proteins and Enzymes
• CD31/PECAM-1 ELISAs