Are the following monoclonals purified from ascites or from tissue culture supernatant? NB100-56541, NB100-56705, NB600-1298, NB100-56534, NB100-56505, NBP2-24873, NB600-1107, NBP2-24917, NB100-56524, NB100-56712

Name: IKK epsilon/IKBKE Antibody (72B587)
Brand: Novus Biologicals
SKU: NB100-56524
Price: 329 USD
Availability: Discontinued
Rating: 5 (2 reviews)

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Accepted Answer

These antibodies are all purified from tissue culture supernatant.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Immunogen	This antibody was raised against a synthetic peptide corresponding to amino acids 175-188 of human IKKi/e protein.
Specificity	The IKK epsilon amino acid sequence used as immunogen is 100% homologous with TBK1 (TANK-binding kinase 1).
Isotype	IgG2a Kappa
Clonality	Monoclonal
Host	Mouse
Gene	IKBKE
Purity	Protein G purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer	PBS containing 0.05% BSA
Preservative	0.05% Sodium Azide
Concentration	0.5 mg/ml
Purity	Protein G purified

Publications using NB100-56524	Applications	Species
Khan KA TRK-Fused Gene (TFG), a protein involved in protein secretion pathways, is an essential component of the antiviral innate immune response PloS Pathog 2021-01-07 [PMID: 33411856]
Peant B, Gilbert S, Le Page C et al. IkB-Kinase-epsilon (IKKE) over-expression promotes the growth of prostate cancer through the C/EBP-B dependent activation of IL-6 gene expression. Oncotarget. 2017-02-28 [PMID: 27577074] (WB, IP, Human)	WB, IP	Human
Klarquist J, Hennies CM, Lehn MA et al. STING-mediated DNA sensing promotes antitumor and autoimmune responses to dying cells. J Immunol. 2014-12-15 [PMID: 25385820] (CoIP)	CoIP
Nakhaei P, Sun Q, Solis M et al. IkB kinase epsilon-dependent phosphorylation and degradation of X-linked inhibitor of apoptosis sensitizes cells to virus-induced apoptosis. J Virol. 2012-01-01 [PMID: 22072751] (WB) Details: WB (TBk1, Ikke or scrambled siRNA transfected A549 cells), Fig 5A. Note: The specificity of the Ikke/Ikki/TBk1 antibody was siRNA validated with Ikke and TBk1 siRNA transfected A549 cells by WB.	WB
Perwitasari O, Cho H, Diamond MS et al. Inhibitor of kB kinase epsilon (IKK(epsilon)), STAT1, and IFIT2 proteins define novel innate immune effector pathway against West Nile virus infection. J Biol Chem. 2011-12-30 [PMID: 22065572] (WB, Mouse) Details: Product cited: IKK-epsilon/IKKi/TBK1 (IMG-270A). 1. WB: IKKe-/- knockout and WT mouse embryonic fibroblasts (MEF), Fig 1B. 2. WB: IKKe-/- knockout mouse and WT spleen cells: Fig 6B. Note: IKK-epsilon/IKKi/TBK1 (IMG-270A) was knockout mouse validated in IK	WB	Mouse
Vandevenne Patricia, Lebrun Marielle, El Mjiyad Nadia et al. The varicella-zoster virus ORF47 kinase interferes with host innate immune response by inhibiting the activation of IRF3. PLoS One. 2011-01-01 [PMID: 21347389] (WB)	WB
Solis Mayra, Romieu-Mourez Raphaelle, Goubau Delphine et al. Involvement of TBK1 and IKKepsilon in lipopolysaccharide-induced activation of the interferon response in primary human macrophages. Eur J Immunol. 2007-02-01 [PMID: 17236232] (IP, Human)	IP	Human
Aksoy E, Albarani V, Nguyen M et al. Interferon regulatory factor 3-dependent responses to lipopolysaccharide are selectively blunted in cord blood cells. Blood. 2007-04-01 [PMID: 17138826] (WB, Human) Details: IMG-139A (TBk1) and IMG 270A (Ikke/Ikki): [WB (Figure S1), human newborn cord blood monocyte-derived dendritic cells].	WB	Human
Bibeau-Poirier A, Gravel SP, Clement JF et al. Involvement of the IkappaB kinase (IKK)-related kinases tank-binding kinase 1/IKKi and cullin-based ubiquitin ligases in IFN regulatory factor-3 degradation. J Immunol. 2006-10-15 [PMID: 17015689] (WB) Details: WB (Figure 5).	WB
Yang Mei-Ling, Doyle Hester A, Gee Renelle J et al. Intracellular protein modification associated with altered T cell functions in autoimmunity. J Immunol. 2006-10-01 [PMID: 16982891] (Mouse)		Mouse
Gravel Simon-Pierre, Servant Marc J. Roles of an IkappaB kinase-related pathway in human cytomegalovirus-infected vascular smooth muscle cells: a molecular link in pathogen-induced proatherosclerotic conditions. J Biol Chem. 2005-03-04 [PMID: 15619605] (ICC/IF)	ICC/IF
Chariot A, Leonardi A, Muller J et al. Association of the adaptor TANK with the I kappa B kinase (IKK) regulator NEMO connects IKK complexes with IKK epsilon and TBK1 kinases. J Biol Chem. 2002-10-04 [PMID: 12133833] (WB)	WB
Show All 12 Publications. Collapse Publications.

	Verified Customer 01/08/2018
Application:	WB
Species:	Human

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