xCT Antibody 0.1 mg

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Western Blot: xCT Antibody [NB100-59735] - Analysis of SLC7A11 using this antibody at 0.02 ug/ml in Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Ordering Information: NB100-59735

  • Catalog Number
    NB100-59735
  • Displayed Format
    Unconjugated
  • Size(s)
    0.1 mg
  • Availability
  • Price
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xCT Antibody Summary

Species Human, Rat
Tested Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Gene
SLC7A11
Purity
Immunogen affinity purified
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xCT Antibody Details

Epitope
KGQTQNFKDAFSGRD
Immunogen
Synthetic peptide, C-KGQTQNFKDAFSGRD, representing the internal region of the sequence according to NP_055146.1

Species Reactivity

Cross reacts with Human, Rat. Expected from sequence similarity: Dog

Applications/Dilutions

Dilutions
  • Western Blot 0.02-0.06ug/ml
  • Peptide ELISA detection limit 1:128000
Application Notes
Western blot: Approx 50kDa band observed in Human Spleen and Human Thymus lysates (calculated MW of 55.4kDa according to NP_055146.1). A customer found positive results in WB on Rat spinal cord.
Positive Controls
Publications
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NB100-59735 in the following applications:

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    Publications

    xCT Antibody (NB100-59735) has been mentioned in at least 1 publication.
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    Packaging, Storage & Formulations

    Storage
    Store at -20C. Avoid freeze-thaw cycles.
    Buffer
    Tris-saline [pH7.3] with 0.5% BSA
    Unit Size
    0.1 mg
    Concentration
    0.5 mg/ml
    Preservative
    0.02% Sodium Azide
    Limitations
    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

    Bioinformatics

    Gene Symbol SLC7A11
    Entrez
    Uniprot

    Alternate Names for xCT Antibody

    • Amino acid transport system xc-
    • Calcium channel blocker resistance protein CCBR1
    • cystine/glutamate transporter
    • Member 11 (Slc7a11)
    • Solute carrier family 7 member 11
    • solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
    • xCTCCBR1
    Show More

    Related Products by Gene

    Background

    SLC7A11 is a member of a heteromeric Na(+)-independent anionic amino acid transport system highly specific for cystine and glutamate. In this system, designated system Xc(-), the anionic form of cystine is transported in exchange for glutamate.[supplied by OMIM]

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    Publications for xCT Antibody (NB100-59735) (1)

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    Product General Protocols

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    Video Protocols

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    FAQs for xCT (2)

    1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
      • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
    2. I was wondering what does Format 7C mean as far as anitbodies go?
      • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

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    Bioinformatics Tool for xCT

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    Blogs on xCT

    Check out the latest blog posts on xCT.
    xCT: The Membrane's Gatekeeper
    xCT is an obligate, electroneutral, membrane-bound anionic transporter responsible for regulating particular amino acid gradients via their transport through plasma membrane. The antiporter xCT superficially resembles an ion channel and preferentially...  Read full blog post.

    xCT: Amino Acid Transport and Disorders of the Central Nervous System
    xCT, encoded by the gene SLC7A11, is a member of the heterodimeric amino acid transporter family. Proteins within this family are linked to one another via a disulphide bond to form heterodimers consisting of one light subunit and one heavy subunit (1...  Read full blog post.

    xCT: Friend or Foe?
    There are two opposing sides to the controversial cysteine/glutamate antiporter. On one hand, it can be viewed a guardian of the cell, protecting it from the damaging oxidative stress that can cause cell death and even cancer. But, conversely, it has ...  Read full blog post.

    Glutathione and xCT: Chemoresistance in Tumor Cells
    Glutathione, called GSH in its reduced form and GSSG or L(-)-Glutathione in its oxidized form, is an endogenous antioxidant found in most cells in the body. Glutathione's functions include detoxifying xenobiotics from the body, assisting in membrane t...  Read full blog post.