pan RhO/RAC/CDC42 Antibody (NB100-91270)

pan RhO/RAC/CDC42 Antibody (NB100-91270) (0.1 ml)

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	Catalog Number	NB100-91270
	Size(s)	0.1 ml	Price:
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pan RhO/RAC/CDC42 Antibody Summary:

Species:	Hu, Mu, Rt	Gene:	RHOA
Applications:	WB, IHC-Fr, IHC-P	Purity:	Whole antisera
Clonality:	Polyclonal	Host:	Rabbit
Specificity:	RhO family.
Publications:	NB100-91270 has at least 5 Background References

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Primary Antibodies
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pan RhO/RAC/CDC42 Antibody Details:

Immunogen:	A synthetic peptide as a part of human/rat/mouse/chicken/dog/fr og (and more species) Rho-related GTP-binding proteins (RhOA, RhOB, RhOC, RhOF, RhOQ) and RAC1, RAC2, RAC3 and CDC42 conjugated to an immunogenic carrier protein was used as the immunogen.

Species Reactivity:

Human, Rat and Mouse.

Applications:

Uses:	Immunohistochemistry-Paraffin/Frozen and Western Blot.
Dilutions:	Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot 1:300-1:3000
Unit Size:	0.1 ml
Concentration:	lyoph

Packaging:

Storage:	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Buffer:	Lyophilized Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. When reconstituting, glycerol (1:1) may be added for an additional stability.
Preservative:	No Preservative
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: RHOA

Entrez:	387 (Human)
	11848 (Mouse)
	117273 (Rat)
	388 (RHOB)
	389 (RHOC)

Related Genes:

Background:

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants.

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pan RhO/RAC/CDC42 Antibody Images (4)

	Western Blot: RhO Antibody [NB100-91270] - WB on rat brain lysate using Rabbit antibody to pan RhO (A, B, C, F, Q), RAC (1, 2, 3) and CDC42: whole serum NB100-91270 at 1 : 1000 dilution.

	Immunofluorescent: RhO Antibody [NB100-91270] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to pan RhO (A, B, C, F, Q), RAC (1, 2, 3) and CDC42: whole serum NB100-91270 at 1: 2000 dilution. DAPI counter stained.

	Immunohistochemistry: RhO Antibody [NB100-91270] - IHC on rat midbrain using Rabbit antibody to pan RhO (A, B, C, F, Q), RAC (1, 2, 3) and CDC42: whole serum NB100-91270 at 1 : 1000 dilution.

	Immunofluorescence: pan RhO/RAC/CDC42 Antibody [NB100-91270] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to pan RhO (A, B, C, F, Q), RAC (1, 2, 3) and CDC42: whole serum at 1: 2000 dilution. DAPI counter stained.