pan Actin Antibody (NB100-74340)
pan Actin Antibody (mAbGEa)
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Distributor Data...
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anti-Actin alpha/gamma, anti-ACTA antibody, anti-pan Actin antibody, anti-NEM3 antibody, anti-NEM2 antibody, anti-NEM1 antibody, anti-MPFD antibody, anti-CFTDM antibody, anti-CFTD1 antibody, anti-CFTD antibody, anti-ASMA antibody, anti-alpha skeletal muscle Actin antibody, anti-alpha Actin 1 antibody, anti-Actin antibody, anti-Actin alpha skeletal muscle antibody, Anti-Actin, anti-ACTA1 antibody, |
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pan Actin Antibody (mAbGEa) Summary: | Species: | Bv, Dr, Hu, Mu, Pl, Rt, Sh, Xp, Ye, Ze |
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| Applications: | ELISA, WB |
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| Clonality: | Monoclonal |
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| | Gene: | ACTA1 |
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| Purity: | Ascites |
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| Host: | Mouse |
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| | Specificity: | This antibody has shown cross-reactivity with actin 1, 2, 3, 4, 7, 8, 11 and 12. |
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| Publications: | Antibody has at least 2 Background References. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional pan Actin Products pan Actin Antibody (mAbGEa) Details: | Clone: | mAbGEa |
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| Isotype: | IgG1 |
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| Immunogen: | Purified Arabidopsis actin protein. |
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Yeast, Xenopus laevis, Drosophila, Zebra Fish, Human, Sheep, Mouse, Rat, Bovine and Arabidopsis. |
| Uses: | ELISA and Western Blot In WB detects a 45 kDa protein representing actin. |
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| Dilutions: | ELISA ,
Western Blot 1:1000 |
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| Unit Size: | 0.1 ml |
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| Concentration: | This product is unpurified. Concentration is not relevant. |
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Packaging: | Storage: | Store at -20 °C. Avoid freeze-thaw cycles. |
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| Preservative: | 0.05% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.
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Images (2) Reviews (1) Related Products (10) Exploring pan Actin Working with pan Actin
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Jump to: Publications, Images, Reviews, Ask a Question
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Working with pan Actin
pan Actin Antibody (mAbGEa) Images (2) Western Blot: pan Actin Antibody (mAbGEa) [NB100-74340] - illustrates Western blot detection of actin in MDCK cell extract using NB100-74340 |  Western Blot: Actin Antibody (mAbGEa) [NB100-74340] - MDCK cell extract |
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Reviewed by:
Anonymous 11/18/2009
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Application:
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Western Blot
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Sample Tested:
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Baking Yeast - Saccharomyces Cerevisiae
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Sample Loaded:
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Control Lane - #7 is 1ug
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Species:
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Yeast
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Lot:
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793-110
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Blocking Buffer:
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powdered milk in PBS
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Concentration:
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3%
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Blocking Time:
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30 minutes
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Blocking Temperature:
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Room temp Celsius
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Dilution Ratio:
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1/1000
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Incubation Time:
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Overnight
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Incubation Temperature:
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4 Celsius
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Incubation Dilution Buffer:
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PBS + 2% BSA
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Description:
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Goat anti mouse HRP
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Manufacturer Catalog #:
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BIO RAD 1706516
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Dilution Ratio:
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1/2000
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Method:
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DAB
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Exposure Time:
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~1 minute
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Positive Control:
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Purified yeast actin (S. cerevisiae)
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Negative Control:
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No actin
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Did you observe bands?:
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Yes
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Specific Bands:
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~45 kDa
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Non-specific Bands:
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0 kDa
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Publications (2)
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Jump to: Primary Antibodies, Secondary Antibodies
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Related ProductsPrimary Antibodies (10)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| pan Actin Antibody (AC-40) | NB120-11003 | Am, Bv, Ca, Ch, GP, Gt, Hu, Mu, Po, Rb, Rt, Sh | DB, ELISA, IF, IHC-P, WB | (1) | (6) | | | | pan Actin Antibody (EP184E) | NB110-55441 | Hu, Mu, Rt | FACS, ICC, IHC-P, IP, WB | (4) | (1) | | | | pan Actin Antibody (ACTN05 (C4)) | NB600-535 | Ca, Ch, Hu, Mu, Rb, Rt | IF, IHC-P, IP, WB | (1) | (2) | | | | pan Actin Antibody | NBP1-61498 | Hu, Mu, Rt | ELISA, IF, IHC, WB | (3) | | | | | pan Actin Antibody (SPM161) | NBP1-74558 | Bv, Ch, Hu, Mu, Rt | ELISA, FACS, IHC-P, IP, WB | (1) | (1) | | | | ... and 5 more. See All » |
Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Mouse IgG Antibody [Biotin] | NB720-B | Mu | ELISA, IHC-P, WB | | | | | | Mouse IgG Antibody [HRP] | NB720-H | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [Alkaline Phosphatase] | NB720-AP | Mu | ELISA, ICC, IHC-P, WB | | | | | | Mouse IgG Antibody [FITC] | NB720-F | Mu | ICC, IHC-P | | (2) | | | | Mouse IgG Antibody | NB120-7056 | Bv(-), Ch(-), Eq(-), Gp(-), Gt(-), Ha(-), Hu(-), Mu, Rb(-), Rt(-), Sh(-) | ELISA, IHC-P, IP, WB | | | | | | See All » |
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