Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
A synthetic peptide as a part of sequestosome-1 (ubiquitin-binding protein p62, p60, EBIAP) conjugated to immunogenic carrier protein has been used as the immunogen.
Localization:
subcellular location: cytoplasm. late endosome. nucleus. note=sarcomere. in cardiac muscles localizes to the sarcomeric band. localizes to late endosomes. may also localize to the nucleus.
Species Reactivity:
Human.
Applications:
Uses:
Immunohistochemistry-Paraffin/Frozen and Western Blot
Dilutions:
Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot 10-50 ug/ml
Reconstitute in 0.5 ml of sterile water. Centrifuge to remove any insoluble material. When reconstituting, glycerol (1:1) may be added for an additional stability.
Packaging:
Storage:
Store at 4 °C short term. Aliquot and store at -20 °C long term. Avoid freeze-thaw cycles.
Buffer:
Lyophilized in PBS
Preservative:
No Preservative
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Adapter protein which binds ubiquitin and may regulate the activation of nfkb1 by tnf-alpha, nerve growth factor (ngf) and interleukin-1. may play a role in titin/ttn downstream signaling in muscle cells. may regulate signaling cascades through ubiquitination. may be involved in cell differentiation, apoptosis, immune response and regulation of k(+) channels.