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mTOR Antibody (NBP1-31523)

mTOR Antibody

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mTOR Antibody Summary:
Species:Hu
Applications:WB
Clonality:Polyclonal
Gene:MTOR
Purity:Affinity purified
Host:Rabbit
 
 
Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur.
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mTOR Antibody Details:
Isotype:IgG
Immunogen:Recombinant protein fragment contain a sequence corresponding to a region within amino acids 2386 and 2547 of Human FRAP1
Localization:Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side, Golgi apparatus membrane, Peripheral membrane protein, Cytoplasmic side, Mitochondrion outer membrane, Peripheral membrane protein, Cytoplasmic side
Species Reactivity:

Tested in human. Not yet tested in any other species.

Applications:
Uses:WB. The usefulness of this product in other applications has not been determined.
Dilutions:Western Blot 1:500-1:2000
Unit Size:0.1 ml
Concentration:0.32 mg/ml
Packaging:
Storage:Store at -20 °C. Avoid freeze-thaw cycles.
Buffer:0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7.0).
Preservative:0.01% Thimerosal
Limitations:This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
Gene Symbol: MTOR
 
Background:

The mammalian target of rapamycin (mTOR) is a major effector of cell growth and proliferation via the regulation of protein synthesis. mTOR is composed of an N-term; 20 tandem repeats- HEAT which are implicated in protein-protein interactions and a C-term; which includes a FAT domain, a FBR domain, a kinase domain, a NDR domain and a FATC domain. The FATC domain is essential to mTOR activity and the deletion of a single amino acid from this domain abrogates the activity. mTOR can be auto-phosphorylated via its intrinsic serine/threonine kinase activity. mTOR regulates protein synthesis through the phosphorylation and inactivation of the repressor of mRNA translation 4E-BP1 and though the phosphorylation and activation of S6 kinase. RAPTOR (regulatory associated protein of TOR) is a positive regulator of TOR. Other known mediators of mTOR include PI3-K and ATK from the insulin pathway.
Images (1)
  • Western Blot: mTOR Antibody [NBP1-31523] - Western blot analysis of NBP1-31523 on 293T, with 5% SDS PAGE, diluted at 1:500.

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mTOR Antibody Images (1)
Western Blot: mTOR Antibody [NBP1-31523] - Western blot analysis of NBP1-31523 on 293T, with 5% SDS PAGE, diluted at 1:500.
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