mTOR Antibody

Species: Rt, Mu, Hu Applications: WB Clonality: Polyclonal	Gene: MTOR Purity: Immunogen affinity purified Host: Rabbit
Specificity: This is specific for Mammalian Target of Rapamycin (mTOR).
See All mTOR Products See All mTOR Related Products: Antibody Packs Peptides and Proteins Primary Antibodies RNAi
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Immunogen:	The epitope recognized by this antibody maps to a region between residue 200 and 250 of human Mammalian Target of Rapamycin using the numbering given in entry NP_004949.1 (GeneID 2475).

Entrez:	2475 (Human)
	56717 (Mouse)
	56718 (Rat)
Swiss Prot:	NP_004949.1 (Human)
Related Genes:	AKT1, EIF4EBP1, RPS6KB1, PSMC4, PIK3R1, PIK3CB, PIK3CG, INS, TSC2, EIF4E, UBASH3B, ANXA6, ELF1, MAPK1, TSC1, PTEN, MAPK3, VEGFA, RPS6, EGFR, EPHB2, CRTC2, KRAS, PRKAA2, PRKAB1, RPTOR, RHEB, IRS1, HRAS1, CCND1, MDK, EIF4G1, CDKN1A, HIF1A, TNF, TRP53, TP53, RORC, EEF2, RICTOR

FKBP 12 rapamycin associated protein (FRAP) is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 235 kDa protein (symbolized RAFT 1) with significant homology to the Saccharomyces cerevisiae protein TOR 1 and has been shown to associate with the immunophilin FKBP 12 in a rapamycin-dependent fashion. The FKBP 12 rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP 12 rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.

Reactivity:	Human, Rat, and Mouse.

Uses:	Western analysis: an overnight incubation at 4 degrees Celcius is recommended for primary antibody use. mTOR is ~235 kDa and optimally requires detergent and extended Western blotting transfer times. For example from 5% acrylamide gels to PVDF: 50V for 180 min. in CAPS buffer with 10% MeOH and 0.1% SDS was found to be optimal. Immunohistochemistry/Immunocytochemistry is not recommended. Please use NB 100-240. Suggested working dilutions: * Western Blot 1:1,000-1:10,000 Immunocytochemistry: Use NB 100-240 instead Immunoprecipitation: Use NB 100-240 instead *The investigator should determine the optimal working dilution for a specific application. Nuclear extracts are recommended.
Dilutions:	Western Blot 1:1000-1:10000
Concentrations:	1 mg/ml
Positive Controls:	1 positive controls

Buffer:	Tris-citrate/phosphate buffer, pH 7 to 8
Storage:	Store at 4C. Do not freeze.
Preservative:	0.1% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.