mTOR Antibody

Protein Type: Peptide Species: Hu Applications: WB Clonality: Polyclonal	Gene: MTOR Purity: Immunogen affinity purified Host: Rabbit
Specificity: mTOR Publications: Antibody has not yet been mentioned in a publication.
See All mTOR Products See All mTOR Related Products: Antibody Packs Lysates Peptides and Proteins Primary Antibodies RNAi
*Note not all species have been tested for reactivity. Only those species listed have been tested. We cannot make any guarantees about additional reactivities or cross reactivities beyond those that have been tested. This antibody may or may not react with other species.

Isotype:	IgG
Immunogen:	Peptide corresponding to amino acids 2-18 of human mTOR

Entrez:	2475 (Human)
Related Genes:	AKT1, EIF4EBP1, RPS6KB1, PSMC4, PIK3R1, PIK3CB, PIK3CG, INS, TSC2, EIF4E, UBASH3B, ANXA6, ELF1, MAPK1, TSC1, PTEN, MAPK3, VEGFA, RPS6, EGFR, EPHB2, CRTC2, KRAS, PRKAA2, PRKAB1, RPTOR, RHEB, IRS1, HRAS1, CCND1, MDK, EIF4G1, CDKN1A, HIF1A, TNF, TRP53, TP53, RORC, EEF2, RICTOR

mTOR (mammalian target of rapamycin, FK506 binding protein 12-rapamycin associated protein 1 (FRAP1)) is a 289 kD member of the PI3 kinase family, containing, HEAT, FRB, FATC domains. mTOR is expressed in the nucleus and cytoplasm and is a target for cell-cycle arrest and the immunosuppressive effects of FKBP12-rapamycin complex. mTOR controls ribosome biogenesis, cell growth, and acts as an ATP sensor. mTOR is repressed by nutrient deprivation and mitochondrial uncoupling. Association of raptor negatively regulates mTOR kinase activity, also inhibited by tuberin-hamartin tumor suppressor complex. mTOR is modified by phosphorylation and associates with FKBP12, rapamycin, p70 S6 kinase, 4E-BP1, raptor, CLIP-170, tuberin, and hamartin. The Poly6199 antibody recognizes human mTOR and has been shown to be useful for Western blotting.

Reactivity:	Human.

Uses:	Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10-20 mul per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Dilutions:	Western Blot

Buffer:	PBS [pH 7.2]
Storage:	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Preservative:	0.09% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.