mTOR Antibody (NB110-56996)
mTOR Antibody (Y391)
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anti-FK506 binding protein12-rapamycin associated protein 1 antibody, anti-RAPT1 antibody, anti-Rapamycin target protein antibody, anti-RAFT1 antibody, anti-mammalian target of rapamycin antibody, anti-FRAP2 antibody, anti-FRAP1 antibody, anti-FRAP antibody, anti-FLJ44809 antibody, anti-FKBP12 rapamycin complex associated protein antibody, anti-FK506 binding protein12-rapamycin associated protein 2 antibody, |
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mTOR Antibody (Y391) Summary: | | Gene: | MTOR |
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| Purity: | IgG purified |
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| Host: | Rabbit |
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| | | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional mTOR Products mTOR Antibody (Y391) Details: | Clone: | Y391 |
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| Isotype: | IgG |
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| Immunogen: | A synthetic peptide corresponding to residues near the C-term of PI3K/PI4K domain of human mTOR/FRAP was used as an immunogen |
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Reacts in Human, Mouse, Rat. |
| Uses: | This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 289kDa. |
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| Dilutions: | Western Blot 1:1000-2000, Immunohistochemistry-Paraffin 1:250-500, Immunocytochemistry 1:50-100, Immunoprecipitation 1:50, Flow Cytometry 1:80 |
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| TMW: | 289 kDa |
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| Unit Size: | 0.1 ml |
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| Concentration: | Please see the vial label for concentration. |
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| Positive Controls: | 1 Positive Controls |
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Packaging: | Storage: | Store at -20 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
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| Preservative: | 0.01% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: The mammalian target of rapamycin (mTOR) is a major effector of cell growth and proliferation via the regulation of protein synthesis. mTOR is composed of an N-term; 20 tandem repeats- HEAT which are implicated in protein-protein interactions and a C-term; which includes a FAT domain, a FBR domain, a kinase domain, a NDR domain and a FATC domain. The FATC domain is essential to mTOR activity and the deletion of a single amino acid from this domain abrogates the activity. mTOR can be auto-phosphorylated via its intrinsic serine/threonine kinase activity. mTOR regulates protein synthesis through the phosphorylation and inactivation of the repressor of mRNA translation 4E-BP1 and though the phosphorylation and activation of S6 kinase. RAPTOR (regulatory associated protein of TOR) is a positive regulator of TOR. Other known mediators of mTOR include PI3-K and ATK from the insulin pathway.
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Images (2) Reviews (0) Related Products (45) Exploring mTOR Working with mTOR
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Jump to: Publications, Images, Protocols/Procedures, Ask a Question
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Working with mTOR
mTOR Antibody (Y391) Images (2) Immunohistochemistry: mTOR/FRAP Antibody (Y391) [NB110-56996] - Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using anti-mTOR/FRAP (cat. # 110-56996). |  Western Blot: mTOR/FRAP Antibody (Y391) [NB110-56996] - Western blot analysis of anti-mTOR/FRAP (cat. # 110-56996), dilution 1:2,000. A: Hela B: MCF-7 |
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Publications (1)mTOR Antibody (Y391) Background References:- Hay, N.; Sonenberg, N. :Genes Dev. 18: 1926-1945, 2004.
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Jump to: Positive Controls, Antibody Packs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
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Related ProductsPositive ControlsAntibody Packs (1)Lysates (1)Peptides and ProteinsPrimary Antibodies (41)| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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RNAi (1)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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