Chromatin Immunoprecipitation Protocol (NB21-2044)
Chromatin Immunoprecipitation Protocol
Cell Fixation and Preparation
1.Begin with a cell culture that has reached 80% confluency.
2. Add formaldehyde to a final concentration of 1% in growth media and incubate for 10 minutes at room temperature.
Histone H4 [ac Lys8] Antibody (NB21-2044)
Rabbit Polyclonal Anti-Histone H4 [ac Lys8] Antibody-ChIP Grade. H4K8ac. Tested Applications: Western Blot, ChIP, Dot Blot, ICC. Tested Reactivity: Human, Mouse, C. elegans.
http://www.rndsystems.com/pdf/msds/2044-cd.pdf PDF MSDS