Chromatin Immunoprecipitation Protocol (NB21-2044)
Chromatin Immunoprecipitation Protocol
Cell Fixation and Preparation
1.Begin with a cell culture that has reached 80% confluency.
2. Add formaldehyde to a final concentration of 1% in growth media and incubate for 10 minutes at room temperature.
Histone H4 [ac Lys8] Antibody (NB21-2044)
Rabbit Polyclonal Anti-Histone H4 [ac Lys8] Antibody-ChIP Grade. H4K8ac. Tested Applications: Western Blot, ChIP, Dot Blot, ICC. Tested Reactivity: Human, Mouse, C. elegans.
http://www.rndsystems.com/pdf/msds/2044-cd.pdf PDF MSDS
Western Blot protocol specific for H4K8ac antibody (NB21-2044)
Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 10 ug of histone preps per lane.
2. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.