Novus Biologicals, Inc PO Box 802 Littleton, CO 80160 Phone:1-888-506-6887 or 303-730-1950 Fax: 303-730-1966 Email: novus@novusbio.com     Chicken Polyclonal anti-HA tag antibody   Catalog Number: NB600-361      Price: 245.00      Background: Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.   Alternate Names: anti-HA epitope tag antibody, anti-HA1 antibody, anti- HA2 antibody, anti- hemagglutinin antibody   Host: Chicken   Research Areas: Epitope Tags and Markers, Epitope Tags and Markers, HA Epitope Tags   Immunogen: Chickens were immunized with HA cleavage site (YPYDVPDYA) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Chicken anti- human HA affinity purified antibodies were coupled to peptide immobilized on solid support.   Specificity: Antibody recognizes the HA cleavage site.   Uses: Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 Cytochemistry 1:100-1:400 *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot, and immunocytochemistry. Other applications have not been tested   Dilutions: Suggested working dilutions * ELISA 1:1000-1:30000, Western Blot 1:1000-1:30000, Immunocytochemistry 1:100-1:400, * Investigator should determine optimal working dilutions.   Packaging: 0.1 ml Immunogen affinity purified Chicken antisera.   Buffer: Phosphate Buffered Saline, pH 7.2   Preservative: 0.09% Sodium Azide   Storage: Store at 4C. Do not freeze.   Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.   Product Specific References: 1. Bedard, J. E. J., Purnell, J. D., and Ware, S. M. (2007) Nuclear import and export signals are essential for proper cellular trafficking and function of ZIC3, 16, 187-198. 2. Wang, J., Shao, Y., Bennett, T. A., Shankar, R. A., Wightman, P. D., and Reddy, L. G. (2006) The Functional Effects of Physical Interactions among Toll-like Receptors 7, 8, and 9, 281, 37427-37434.   © 2009 NOVUS BIOLOGICALS | www.novusbio.com | 888-506-6887 or 303-730-1950