Positive Controls and Cell Lysates, Phospho-Specific
Uses:
This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. This lysate is prepared in denaturing buffer without dissociating agent (i.e. no 2-mercaptoethanol or dithiothreitol has been added). Other applications have not been tested.
Positive Controls:
Jurkat is only applicable as a positive control for specific antibodies.
Packaging:
0.5mg whole cell lysate
Concentration:
1.0 mg/ml
Buffer:
50mM Tris HCI with 2% SDS, 10% Glycerol, 0.005% Bromophenol blue [pH 6.8]
Preservative:
No preservative
Storage:
Store at -80C. Avoid freeze-thaw cycles.
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis.
Figure. Coommassie stained SDS-PAGE of 100 ?g
of Human derived Jurkat Whole Cell Lysate (Readyto-
Use) separated in a 4-20% gradient gel under
non-reducing conditions (lane 1). Molecular weight
standards are shown in lane 2.