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Jurkat Whole cell lysate


Jurkat whole cell lysate (Positive control)



Catalog Number: NB800-PC2
Price: $145.00 Add to cart
 

Research Areas:

Positive Controls and Cell Lysates, Phospho-Specific



Uses:

This lysate is prepared as a positive control for separation by SDS-PAGE and subsequent Western blot analysis. This lysate is prepared in denaturing buffer without dissociating agent (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Other applications have not been tested.



Positive Controls:

Jurkat is only applicable as a positive control for specific antibodies.



Packaging:

0.5mg whole cell lysate



Concentration:

1.0 mg/ml



Buffer:

50mM Tris HCI with 2% SDS, 10% Glycerol, 0.005% Bromophenol blue [pH 6.8]



Preservative:

No preservative



Storage:

Store at -80C. Avoid freeze-thaw cycles.



Limitations:

This product is for research use only and is not approved for use in humans or in clinical diagnosis.



Figure. Coommassie stained SDS-PAGE of 100 ?g of Human derived Jurkat Whole Cell Lysate (Readyto- Use) separated in a 4-20% gradient gel under non-reducing conditions (lane 1). Molecular weight standards are shown in lane 2.