Acrosin antibody

Mouse Monoclonal anti-Acrosin (ACR-2)

Catalog Number: NB500-419	Price: $295.00

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Background:

Acrosin is the major proteinase present in the acrosome of mature spermatozoa. It is a typical serine proteinase with trypsin-like specificity. It is stored in the acrosome in its precursor form, proacrosin. The active enzyme functions in the lysis of the zona pellucida, thus facilitating penetration of the sperm through the innermost glycoprotein layers of the ovum. The mRNA for proacrosin is synthesized only in the postmeiotic stages of spermatogenesis. In humans proacrosin first appears in the haploid spermatids.

Alternate Names:

anti-ACR antibody, anti-Acrosin precursor antibody, anti-ACRS antibody, anti-Proacrosin antibody

Host:

Mouse

Research Areas:

Signal Transduction

Immunogen:

Acid extracts of boar spermatozoa were subjected to hydrophobic chromatography and the pooled fraction with reactivity to N-alpha benzoylarginine-4-nitroanilide was used for immunization.

Clone:

ACR-2

Isotype:

IgG1

Specificity:

The antibody ACR 2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa.

Species Reactivity:

Cross-reacts with Pig.Does not cross-react with Cow, Dog or Human.Not yet tested in other species.
Other species have not been tested.

Uses:

Immunocytochemistry, Western Blot
ICC: Use at an assay dependent dilution membrane permeabilization (acetone) essential. The antibody ACR-2 reacts with various forms of porcine acrosin (55, 53, 45 and 35 kDa) in the acrosome of undamaged spermatozoa. Optimal dilutions and concentrations should be determined by the end user.
Other applications have not been tested.

Dilutions:

Suggested working dilutions *
Western Blot ,
Immunocytochemistry
* Investigator should determine optimal working dilutions.

Packaging:

0.1 mg

Concentration:

1 mg/ml

Buffer:

PBS. pH 7.4

Preservative:

15mM Sodium Azide

Storage:

Store at 2-8oC. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20oC. Avoid freeze/thaw cycles.

Limitations:

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.

General References:

This product has been used in:Moos J et al. Protein-protein interactions controlling acrosin release and solubilization during the boar sperm acrosome reaction. Biol Reprod 49:408-15 (1993). Peknicova J & Moos J Monoclonal antibodies against boar acrosomal antigens labelling undamaged acrosomes of spermatozoa in immunofluorescence test. Andrologia 22:427-35 (1990).

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