ABCG8 antibody

Rabbit Polyclonal anti-ABCG8, Sample Size

Catalog Number: NB400-110SS	Price: $100.00
Peptide: NB400-110PEP
Full Size Vial: NB400-110

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Background:

ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. ABCG8, expressed at high levels in the liver and intestine, normally cooperates with ABCG5 to limit intestinal absorption and promote biliary excretion of sterols. The mutated form of this transporter can lead to sterol accumulation and atherosclerosis or sitosterolemia, a rare autosomal recessive disorder, characterized by hyperabsorption of sterols and the inability to excrete sterols into bile.

Alternate Names:

anti-ATP binding cassette sub family G (WHITE) member 8 (sterolin 2) antibody, anti-ATP binding cassette sub family G member 8 antibody, anti-MGC142217 antibody, anti-Sterolin 2 antibody, anti-STSL antibody, anti-ATP binding cassette G8 antibody

Related Diseases:

Atherosclerosis, Coronary Artery Disease

Host:

Rabbit

Research Areas:

ABC Transporters, Lipid and Cardiovascular Research, Focus On: Cholesterol Metabolism

Immunogen:

A synthetic peptide from the N-terminal region of human ABCG8 protein.

Specificity:

Specific for ABCG8.

Reference Sequence:

Q9H221

Localization:

Integral membrane protein; multi-pass membrane protein.

Species Reactivity:

Human. Does not appear to cross react with mouse.
Other species have not been tested.

Uses:

This antibody is useful for Western Blot .
Other applications have not been tested.

Dilutions:

Suggested working dilutions *
Western Blot 1:500
* Investigator should determine optimal working dilutions.

Packaging:

0.025 ml Immunogen affinity purified Rabbit antisera.

Concentration:

1 mg/ml

Preservative:

0.1% sodium azide

Storage:

Store at 4C.  Do not freeze.

Limitations:

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.

Novus Specific References:

1. Kobayashi, A., et al. Efflux of sphingomyelin cholesterol and phosphatidylcholine by ABCG1. J. Lipid Res. 47:1791-1802, 2006.
2. 2. Mathur, S. N., Watt, K. R., and Field, F. J. (2007) Regulation of intestinal NPC1L1 expression by dietary fish oil and docosahexaenoic acid, J. Lipid Res. 48: 395-404.

General References:

1.Patel, S.B., et al. Two genes that map to the STSL locus cause sitosterolemia: genomic structure and spectrum of mutations involving sterolin-1 and sterolin-2, encoded by ABCG5 and ABCG8,respectively. Am. J. Hum. Genet., 69: 278-290. 2001
2.Garcia, C.K., et al. Autosomal recessive hypercholesterolemia caused by mutations in a putative LDL receptor adaptor protein. Science, 292: 1394-1398. 2001
3.Schmitz, G., et al. Role of ABCG1 and other ABCG family members in lipid metabolism. J. Lipid Res. 42: 1513-1519. 2001
4. Remaley, A., et al. Comparative genome analysis of potential regulatory elements in the ABCG5-ABCG8 gene cluster. BBRC. 295:276-282,2002.

Gene Id:

64241

Gene System:

ABCG8

Western blot analysis of ABCG8, using NB 400-110. Samples: mouse liver microsomes from G5/G8 knockout mice, wildtype human G5/G8, and transgenic human G5/G8.

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