Today's Research.
Tomorrow's Discovery.
Tomorrow's Discovery.
| Datasheet |  |
Procedure Guide | |
ABCA1 Related Products | ||
ABCA1 antibody
Rabbit Polyclonal anti-ABCA1, Sample Size
| Catalog Number: NB400-105SS |
Price:
$100.00
|
| Peptide: NB400-105PEP | |
Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester deposition in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
Alternate Names:
anti-ABC 1 antibody, anti-ABC Transporter 1 antibody, anti-ABC1 antibody, anti-ATP-binding Cassette 1 antibody, anti-ATP-binding Cassette Transporter 1 antibody, anti-CERP antibody, anti-Cholesterol Efflux Regulatory Protein antibody, anti-ABC-1 antibody
Related Diseases:
Tangier Disease, Atherosclerosis, Coronary Artery Disease
Host:
Rabbit
Research Areas:
Astrocyte Marker, Lipid and Cardiovascular Research, ABC Transporters, Golgi Proteins, IHC Grade
Immunogen:
Partial peptide sequence between residues 110-1300 of the human ABCA1 gene.
Specificity:
This antibody is specific for ABCA1.
Reference Sequence:
O95477
Species Reactivity:
NB 400-105 reacts with human, rat, hamster and mouse ABCA1. Other species have not been tested.
Other species have not been tested.
Uses:
NB 400-105 may be used for Western analysis where a band representing ABCA1 is seen at ~220 kDa. Additional non-specific bands are seen at lower molecular weights, but do not interfere with the ABCA1 signal.
Other applications have not been tested.
Dilutions:
Suggested working dilutions *
flow cytometry / FACS analysis ,
immunohistochemistry 1:200,
immunoprecipitation See Krimbou,
Western Blot 1:500-1:1000,
Immunocytochemistry
* Investigator should determine optimal working dilutions.
Packaging:
0.025 ml of affinity purified rabbit antisera.
Concentration:
1 mg/ml
Buffer:
Tris/citrate, pH 7-8.
Preservative:
0.02% sodium azide
Storage:
Store at 4C. Do not freeze.
Limitations:
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.
Novus Specific References:
1. Murthy, S., et al. LXR/RXR activation enhances basolateral efflux of cholesterol in CaCo-2 cells. J. Lipid Research. 43: 1054-1064, 2002. [WB, human]
2. Burgess, J.W., et al. Trypsin-sensitive and lipid-containing sites of the macrophage extracellular matrix bind apolipoprotein A-I and participate in the ABCA-1-dependent cholesterol efflux. JBC Papers in Press. 277(35): 31318-31326, 2002. [WB, human/mouse]
3. Haidar, B., et al. camp induces ABC1 phosphorylation activity and promotes cholesterol efflux from fibroblasts. Journal of Lipid Research. 43(12):2087-2094,2002. [IP, human]
4. Terasaka, N., et al. T-0901317, a synthetic liver X receptor ligand, inhibits development of atherosclerosis in LDL receptor-deficient mice. FEBS Letters. 536: 6-11, 2003. [IHC-P, mouse]
5. Basso, F., et al. Role of the hepatic ABCA1 transporter in modulating intrahepatic cholestrol and plasma HDL cholestrol concentrations. Journal of Lipid Research. 44:296-302, 2003. [WB, mouse]
6. Le Lay, S., et al. Regulation of ABCA1 expression and cholesterol efflux during adipose differentiation of 3T3-L1 cells, J. Lipid Res. 44(8): p. 1499-1507, 2003. [WB, mouse]
7. Khovidhunkit, W., et al. Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: differential role of LXR. Journal of Lipid Research. 44: 1728-1736, 2003. [WB, mouse]
8. Wang, N., et al. A PEST sequence in ABCA1 regulates degradation by calpain protease and stabilization of ABCA1 by apoA-I. Journal of Clinical Investigation.111(1): 99-107, 2003. [IP/WB, human]
9. Escher, G., et al. Expression of Sterol 27-Hydroxylase (CYP27A1) Enhances Cholesterol Efflux. Journal of Biological Chemistry. 278(13):11015-11019, 2003. [WB, human/hamster]
10. Feng, B., et al. ABCA1-mediated Cholestrol Efflux Is Defective in Free Cholesterol-loaded Macrophages. Journal of Biological Chemistry. 277(45):43271-73280, 2002. [WB, mouse]
11. Fukumoto, H., et al. Induction of the Cholesterol Transporter ABCA1 in Central Nervous System Cells by Liver X Receptor Agonists Increases Secreted A Beta Levels. Journal of Biological Chemistry. 277(50):48508-48513, 2002. [WB, human/mouse]
12. Koldamova, R., et al. 22R-Hydroxycholestrol and 9-cis-Retinoic Acid Induce ATP-binding Cassette Transporter A1 Expression and Cholesterol Efflux in Brain Cells and Decrease Amyloid beta Secretion. Journal of Biological Chemistry. 278(15):13244-13256, 2003. [WB/IHC/IF, rat]
13. Sun, Y., et al. Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. Journal of Biological Chemistry. 278(30):27688-37694, 2003. [WB, mouse]
14. Witting, S. et al. Ceramide Enhances Cholesterol Efflux to Apolipoprotein A-I by Increasing the Cell Surface Presence of ATP-binding Cassette Transporter A1. Journal of Biological Chemistry. 278(41):40121-40127, 2003. [WB, mouse]
15. Krimbou, et al. Molecular Interactions between Apolipoprotein E and the ATP-binding Cassette Transporter A1 J Biol Chem. 45: 839-848, 2004. [IP, human]
16. Gaus, K., et al. Visualizing lipid structure and raft domains in living cells with two- photon microscopy. PNAS. 100(26): 15554-15559, 2003. [ICC, mouse/human]
17. Denis, M., et al. Molecular and Cellular Physiology of Apolipoprotein A-I Lipidation by the ATP-binding Cassette Transporter A1 (ABCA1). Journal of Biological Chemistry. 279(9):7384-7394,2004. [IP, human]
18. Bortnick, A: Identification and characterization of rodent ABCA1 in isolated type II pneumocytes. Am J Physiol Lung Cell Mol Physiol 285: L869-L878, 2003. [WB, human]
19. Wagner, B: Promoter-Specific Roles for Liver X Receptor/Corepressor Complexes in the Regulation of ABCA1 and SREBP1 Gene Expression. Molecular And Cellular Biology, Aug. 2003, pp. 5780-5789. [WB, mouse]
20. Sun, Y: Expression of Liver X Receptor Target Genes Decreases Cellular Amyloid beta Peptide Secretion. The Journal of Biological Chemistry. Vol.278, No.30 July 25,pp. 27688-27694, 2003. [WB, mouse]
21. Field, F., et al. LXR/RXR ligand activation enhances basolateral efflux of beta-sitosterol in CaCo-2 cells. Journal of Lipid Research. 45:905-913, 2004. [WB, human]
22. Krimbou, L., et al. Molecular interactions between apoE and ABCA1: impact on apoE lipidation. Journal of Lipid Research. 45:839-848, 2004. [IP, human]
23. Brown III, J., et al. Differential Expression of Cholesterol Hydroxylase in Alzheimer’s Disease. The Journal of Biological Chemistry. 279(33): 34674-34681, 2004. [WB, human]
24. Sahoo, D., et al. ABCA1-dependent lipid effulux to apolipoprotein A-1 mediates HDL particle formation and decreases VLDL secretion from murine hepatocytes. Journal of Lipid Research. 45: 1122-1131, 2004. [WB, rat]
25. Denis, M., et al. Characterization of Oligomeric Human ATP Binding Cassette Transporter A1. The Journal of Biological Chemistry. 279(40):41529-41536, 2004. [IP, human]
26. Gaus, K., et al. Apolipoprotein A-1 interaction with plasma membrane lipid rafts controls cholesterol export from macrophages. The FASEB Journal. 10.1096/fr.03-0486fje., 2004.
27. Koldamova, R., et al. The Liver Receptor Ligand T0901317 Decreases Amyloid Beta Production in Vitro and in a Mouse Model of Alzheimer's Disease. The Journal of Biological Chemistry. 280(6): 4079-4088, 2005. [WB, mouse]
28. Ando, H., et al. Effects of Pravastatin on the Expression of ATP-Binding Cassette Transporter A1. J. Pharmacol. Exp. Ther. 311: 420-425, 2004.
29. Li. A., et al. Differential inhibition of macrophage foam-cell formation and atherosclerosis in mice by PPAR alpha alpha, B/delta, and gamma. J. Clin. Invest. 114: 1564-1576, 2004.
30. Haidar, B., et al. Apolipoprotein A-I Activates Cellular cAMP Signaling through the ABCA1 Transporter. J. Biol. Chem. 279: 9963-9969, 2004. [IP, human]
31. Bared, S., et al. Association of ABCA1 with Syntaxin 13 and Flotillin-1 and Enhanced Phagocytosis in Tangier Cells. Mol. Biol. Cell. 15: 5399-5407, 2004.
32. Dove, D. ACAT1 Deficiency Disrupts Cholesterol Efflux and Alters Cellular Morphology in Macrophages. Arterioscler. Thromb. Vasc. Biol. 25: 128-134, 2004.
33. Agassandian, M., et al. Oxysterols Trigger ABCA1-Mediated Basolateral Surfactant Efflux. Am. J. Respir. Cell Mol. Biol. 31: 227-233, 2004.
34. O'Connell, B., et al. Cellular Physiology of Cholesterol Efflux in Vascular Endothelial cells. Circulation. 110: 2881-2888, 2004.
35. Zeng, L., et al. Sterol-responsive Element-binding Protein (SREBP) 2 Down-regulates ATP-binding Cassette Transporter A1 in Vascular Endothelial Cells: A NOVEL ROLE OF SREBP IN REGULATING CHOLESTEROL METABOLISM. J. Biol. Chem. 279: 48801-48807, 2004. [WB, human]
36. Glaros, E.N., et al. Glycosphingolipid accumulation inhibits cholesterol efflux via the ABCA1/apolipoprotein A-1 pathway. J. Biol. Chem. 280(26): 24515-24523, 2005. [WB, human]
37. Su, Y., et al. Macrophage Apolipoprotein A-I Expression Protects against Atherosclerosis in ApoE-Deficient Mice and Up-regulates ABC Transporters. Molecular Therapy. 0:1-8,2003. [WB, mouse]
38. Schmid, K., et al. Transport of cholesterol across a BeWo cell monolayer: implications for net transport of sterol from maternal to fetal circulation. Journal of Lipid Research. 44: 1909-1918, 2003. [WB, human]
39. Khovidhunkit, W. et al. Endotoxin down-regulates ABCG5 and ABCG8 in mouse liver and ABCA1 and ABCG1 in J774 murine macrophages: defferential role of LXR. Journal of Lipid Research. 44: 1728-1736, 2003. [WB, mouse]
40. Dong, J. Adenovirus-mediated overexpression of sphingomylein synthases 1 and 2 increases the atherogenic potential in mice. Journal of Lipid Research Volume 47, 2006.
41. Zager, R.A., et al. Proximal tubular cholesterol loading after mitochondrial but not glycolytic blockade. Am. J. Physiol. Renal Physiol. 285:1092-1099, 2003.
42. Berfield, A.K., et al. IGF-1 induces rat glomerular mesangial cells to accumulate triglyceride. Am. J. Physiol. Renal Physiol. 290:138-147, 2006.
43. Zhang, C., et al. NO-1886 suppressing atherosclerosis in high-fat-high-sucrose/high-cholesterol fed Bama minipigs is related to upregulating ATP-binding cassette transporter A1. J. Lipid Res. 2006.
44. Dong, J., et al. Adenovirus-mediated overexpression of sphingomyelin synthases 1and 2 increases the atherogenic potenial in mice. J. Lipid Res. 47:1307-1314, 2006.
45. Duong. P.T., et al. Characterization of nascent HDL particles and microparticles formed by ABCA1-mediated efflux of cellular lipids to apoA-I. J. Lipid Res. 47:832-843, 2006.
46. Boisvert, W.A., et al. Leukocyte transglutaminase 2 expression limits atherosclerotic lesion size. Arterioscler. Thromb. Vasc. Biol. 26:563-569, 2006.
47. Forcheron, F., et al. Genes of cholesterol metabolism in human atheroma: overexpression of perilipin and genes promoting cholesterol storage and repression of ABCA1 expression. . Thromb. Vasc. Biol. 25:1711-1717, 2005.
48. Reddy, S.T., et al. ATP-binding cassette transporter 1 participates in LDL oxidation by artery wall cells. 22:1877-1883, 2002.
49. Trogan, E., et al. Gene expression changes in foam cells and the role of chemokine receptor CCR7 during athersclerosis regerssion in Apo-E-deficient mice. PNAS. 103:3781-3786, 2006.
50. Gerbod-Giannone, M-C. et al. TNFa induces ABCA1 through NF-kappaB in macrophage and in phagocytes ingesting apoptotic cells. PNAS. 103:3112-3117, 2006.
51. Gaus, K., et al. Visualizing lipid structure and raft domains in living cells with two-photon microscopy. PNAS 100:15554-15559, 2003.
52. Atshaves, B., et al. SCP-2/SCP-x gene ablation alters lipid raft domains in primary cultured mouse hepatocytes. Journal of Lipid research. 48: 2193-2211, 2007.
53. During, A., et al. Mechanisms of provitamin A (carotenoid) and vitamin A (retinol) transport into and out of intestinal Caco-2 cells. Journal of Lipid Research. 48: 2283-2294, 2007.
54. Hassan, H. et al. Identification of an ABCA1-dependent phospholipid-rich plasma membrane apolipoprotein A-1 binding site for nascent HDL formation: implications for current models of HDL biogenesis. Journal of Lipid Research. 48: 2428-2442, 2007.
55. Hoe, H., et al.The Metalloprotease Inhibitor TIMP-3 Regulates Amyloid Precursor Protein and Apolipoprotein E Receptor Proteolysis. J. Neurosci.27: 10895-10905.Oct 2007.
56. Talukdar, S., et al. Chenodeoxycholic acid suppresses the activation of acetyl-coenzyme A carboxylase-alpha gene transcription by the liver X receptor agonist TO-901317. J. Lipid Res., Dec 2007; 48:2647-2663.
57. Bates, S. R., J.-Q. Tao, et al. (2007). Expression and Biological Activity of ABCA1 in Alveolar Epithelial Cells: 2007-0020OC.
58. Babaev, V. R., H. Ishiguro, et al. (2007). Macrophage Expression of Peroxisome Proliferator Activated Receptor-{alpha} Reduces Atherosclerosis in Low-Density Lipoprotein Receptor Deficient Mice. 116: 1404-1412.
59. Out, R., et al. Coexistence of Foam Cells and Hypocholesterolemia in Mice Lacking the ABC Transporters A1 and G1. Circ. Res. Jan 2008; 102: 113-120.
60. Wang N, Yvan-Charvet L, Lutjohann D, et al. ATP-binding cassette transporters G1 and G4 mediate cholesterol and desmosterol efflux to HDL and regulate sterol accumulation in the brain. FASEB J. 2007 November 26, 2007:fj.07-9944com.
61. Zhu M, Fu Y, Hou Y, et al. Laminar Shear Stress Regulates Liver X Receptor in Vascular Endothelial Cells. Arterioscler Thromb Vasc Biol. March 1, 2008;28(3):527-33. (Western Blot)
62. Hastie C, Masters JR, Moss SE, Naaby-Hansen S. INFgamma reduces cell surface expression of annexin 2 and suppresses the invasive capacity of prostate cancer cells. J Biol Chem. January 22, 2008:M800189200.
63. Jacobs RL, Lingrell S, Zhao Y, Francis GA, Vance DE. Hepatic CTP:Phosphocholine Cytidylyltransferase-{alpha} Is a Critical Predictor of Plasma High Density Lipoprotein and Very Low Density Lipoprotein. J Biol Chem. January 25, 2008; 283(4):2147-55.
64. Yan D, Mayranpaa MI, Wong J, et al. OSBP-related Protein 8 (ORP8) Suppresses ABCA1 Expression and Cholesterol Efflux from Macrophages. J Biol Chem. January 4, 2008; 283(1):332-40.
65. Zhou X, He W, Huang Z, Gotto AM, Jr., Hajjar DP, Han J. Genetic Deletion of Low Density Lipoprotein Receptor Impairs Sterol-induced Mouse Macrophage ABCA1 Expression: A NEW SREBP1-DEPENDENT MECHANISM. J Biol Chem. January 25, 2008; 283(4):2129-38.
66. Henry-Berger J, Mouzat K, Baron S, et al. Endoglin (CD105) Expression Is Regulated by the Liver X Receptor Alpha (NR1H3) in Human Trophoblast Cell Line JAR. Biol Reprod 2008;78(6):968-975.
67. Watanabe, T., K. Nishio, et al. Impact of Salusin-{alpha} and -{beta} on Human Macrophage Foam Cell Formation and Coronary Atherosclerosis. Circulation. 2008; 117: 638-648 p.
68. Ouimet M, Wang M-D, Cadotte N, et al. Epoxycholesterol Impairs Cholesteryl Ester Hydrolysis in Macrophage Foam Cells, Resulting in Decreased Cholesterol Efflux. Arterioscler Thromb Vasc Biol 2008;28(6):1144-1150. (Western Blot)
69. Smoak K, Madenspacher J, Jeyaseelan S, et al. Effects of Liver X Receptor Agonist Treatment on Pulmonary Inflammation and Host Defense. J Immunol. March 1, 2008;180(5):3305-12.
70. Montoudis, A. et al. Intestinal fatty acid binding protein regulates mitochonfrion beta-oxidation and cholesterol uptake. J. Lipid Res. January 30, 2008; Volume 49: 961-972. (WB)
72. Frank PG, Pavlides S, Cheung MWC, et al. Role of caveolin-1 in the regulation of lipoprotein metabolism. Am J Physiol Cell Physiol 2008;295(1):C242-248.
72. Bowden K and Ridgway ND. OSBP Negatively Regulates ABCA1 Protein Stability. J Biol Chem 2008;283(26):18210-18217.
73. Hastie C, Masters JR, Moss SE, Naaby-Hansen S. Interferon-{gamma} Reduces Cell Surface Expression of Annexin 2 and Suppresses the Invasive Capacity of Prostate Cancer Cells. J Biol Chem. May 2, 2008;283(18):12595-603.
74. Kumar R, McClain D, Young R, et al. Cholesterol transporter ATP-binding cassette A1 (ABCA1) is elevated in prion disease and affects PrPC and PrPSc concentrations in cultured cells. J Gen Virol 2008;89(6):1525-1532.
75. Tancevski I, Wehinger A, Demetz E, et al. Reduced Plasma High-Density Lipoprotein Cholesterol in Hyperthyroid Mice Coincides with Decreased Hepatic Adenosine 5'-Triphosphate-Binding Cassette Transporter 1 Expression. Endocrinology 2008;149(7):3708-3712.
76. Mukhamedova N, Escher G, D'Souza W, et al. Enhancing apolipoprotein A-I-dependent cholesterol efflux elevates cholesterol export from macrophages in vivo. J Lipid Res 2008;49(11):2312-2322.
77. Peng D, Hiipakka RA, Dai Q, et al. Antiatherosclerotic Effects of a Novel Synthetic Tissue-Selective Steroidal Liver X Receptor Agonist in Low-Density Lipoprotein Receptor-Deficient Mice. J Pharmacol Exp Ther 2008;327(2):332-342.
78. Paul, V., Meyer, H., Leidl, K., et al. A Novel Enzyme Immunoassay Specific for ABCA1 Protein Quantification in Human Tissues and Cells. J. Lipid Res. October 2008; 49: 2259-2267.
79. Delvecchio CJ, Bilan P, Nair P, et al. LXR-INDUCED REVERSE CHOLESTEROL TRANSPORT IN HUMAN AIRWAY SMOOTH MUSCLE IS MEDIATED EXCLUSIVELY BY ABCA1. Am J Physiol Lung Cell Mol Physiol 2008:90394.92008.
80. Nakanishi S, Vikstedt R, Soderlund S, et al. Serum, but not monocyte macrophage foam cells derived from low HDL-C subjects, displays reduced cholesterol efflux capacity. J Lipid Res 2008:M800196-JLR800200.
81. Nandi S, Ma L, Denis M, et al. ABCA1-mediated cholesterol efflux generates microparticles in addition to HDL through processes governed by membrane rigidity. J Lipid Res 2008:M800345-JLR800200.
82. Robichaud JC, Francis GA, and Vance DE. A role for hepatic scavenger receptor class B, type I in decreasing high-density lipoprotein levels in mice that lack phosphatidylethanolamine N-methyltransferase. J Biol Chem 2008:M807433200.
83. Cartagena, C., Ahmed, F., Burns, M., et al. Cortical Injury Increases Cholesterol 24S Hydroxylase(Cyp46) Levels in the Rat Brain. J. of Neurotrauma. Sept. 2008:1087-1098.
Notes:
It is important not to boil the sample before loading onto a gel. Boiling can cause aggregation in large proteins, resulting in the proteins inability to enter the gel. Post-nuclear lysates of 9-cis retinoic acid and 22R-hydroxycholesterol induced raw macrophages were used for the positive control experiments (see image).
____________________________________________________________
Blocking peptide is available or can be made for this item. Lead time is 2 days to 4 weeks. Price is $ 150/0.05 ml (or 50 ug). Please enquire availability (cat# NB 400-105PEP).
Gene Id:
19
Gene System:
ABCA1
Related Rabbit Secondary Antibodies