Acetylcholinesterase antibody

Mouse Monoclonal anti-Acetylcholinesterase (HR2)

Catalog Number: NB300-528	Price: $295.00

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Background:

Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft.

Alternate Names:

anti-Acetylcholine acetylhydrolase antibody, anti-Acetylcholinesterase YT blood group antibody, anti-ACHE antibody, anti-ACHE protein antibody, anti-Apoptosis related acetylcholinesterase antibody, anti-ARACHE antibody, anti-N ACHE antibody, anti-YT antibody, anti-Yt blood group antibody

Host:

Mouse

Research Areas:

Neuroscience, Acetylcholine, Neurotransmission, Neuronal Markers, IHC Grade

Immunogen:

Purified human cerebellar acetylcholinesterase

Clone:

HR2

Isotype:

IgG2b

Specificity:

This antibody does not detect butyrylcholinesterase (BChE).

Localization:

Cell Membrane and Secreted

Species Reactivity:

Cross-reacts with Human, Rabbit, Cow, Cat and Guinea pig. Not yet tested in other species.
Other species have not been tested.

Uses:

Immunohistochemistry (frozen), ELISA, Immunoprecipitation. Immunohistochemical staining of AChE in human brain samples results in staining of nerve fibers and terminals. This antibody cannot be used in Western blot to detect AChE. This antibody is not suitable for FACS.
Other applications have not been tested.

Dilutions:

Suggested working dilutions *
ELISA assay dependent,
immunohistochemistry 1:50,
immunoprecipitation Assay dependent,
Immunohistochemistry-Frozen 1:50
* Investigator should determine optimal working dilutions.

Packaging:

0.2 ml Mouse ascites.

Buffer:

PBS

Preservative:

0.05% sodium azide

Storage:

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Limitations:

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.

General References:

This product has been used in:Rakonczay Z & Brimijoin S Monoclonal antibodies to human brain acetylcholinesterase: properties and applications. Cell Mol Neurobiol 8:85-93 (1988). General / background references:Mesulam MM et al. Immunocytochemical demonstration of axonal and perikaryal acetylcholinesterase in human cerebral cortex. Brain Res 539:233-8 (1991). J. Neural. Transm. 34:139-1450 (1991).

Gene Id:

43

Gene System:

ACHE

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