Acrolein antibody

Rabbit Polyclonal anti-Acrolein

Catalog Number: NB200-556	Price: $295.00
Peptide: NB200-556PEP

Printable Version

PDF Version

Technical Assistance

Alternate Names:

anti-Acrylic amide antibody; anti-Ethylene Carboxamide 2-Propenamide antibody; Anti- 2-Propen-1-one antibody; Anti-2-propenal antibody; Anti- Acraldehyde antibody; Anti-Acrolein antibody; Anti-Acrylic aldehyde antibody

Host:

Rabbit

Research Areas:

Amino Acids, Drugs and other small molecules, IHC Grade

Immunogen:

Acrolein carriers.

Isotype:

IgG

Specificity:

Using a conjugate Acrolein Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds. Acrolein-BSA (ratio 1:1), Malondialdehyde-BSA (1:55), Glyoxal-BSA (1:>50,000), Formaldehyde-BSA (1:>50,000), Glutaraldehyde-BSA (1/>50,000). Crossreactivity ratio is the Acrolein BSA concentration/unconjugated or conjugated analogs concentration at half displacement.

Uses:

For IHC, FFPE samples must perform antigen retrieval (heat method) and de-paraffinization.
Other applications have not been tested.

Dilutions:

Suggested working dilutions *
ELISA 1:5000 - 1:10000,
immunohistochemistry 1:500 - 1:1000,

* Investigator should determine optimal working dilutions.

Packaging:

0.025 mg Immunogen affinity purified Rabbit antisera.

Concentration:

1 mg/ml

Buffer:

PBS, 40% glycerol

Preservative:

No Preservatives

Storage:

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Limitations:

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.

Notes:

For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Printable Version

PDF Version

Technical Assistance

Related Rabbit Secondary Antibodies