ACCN4 antibody

Rabbit Polyclonal anti-ACCN4

Catalog Number: NB110-74734	Price: $295.00

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Background:

FUNCTION: Probable cation channel with high affinity for sodium. These channels have been implicated in synaptic transmission, pain perception as well as mechanoperception.; SUBUNIT: Homotetramer or heterotetramer with other ASIC proteins (Probable).; SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.; TISSUE SPECIFICITY: This gene is predominantly expressed in the pituitary gland. Expressed in brain, spinal chord and dorsal root ganglion (DRG). Expressed by a subset of sensory neurons in the DRG. Expressed by granule cells in the cerebellar cortex. In hippocampus, expression is detected in dentate gyrus granule cells, in pyramidal cells of CA1-CA3 subfields and in interneurons of the striatum oriens and radiatum of all subfields. In cerebral cortex expressed in small, medium and large pyramidal cells in layers 2, 3 and 5 respectively. Also expressed in striatum, globus pallidus, inferior and superior calliculi, amygdala, magnocellular preoptic nucleus, islands of Calleja and large neurons of olfactory tubercules.; DEVELOPMENTAL STAGE: Highly expressed in newborn spinal chord but hardly detected in the cerebellum compared to adult. Expressed at postnatal day 1 in ependymal cells lining the central canal of spinal chord and in motor neurons. In adult, expression decreases in ependymal cells and increases in motor neurons. The number of positive interneurons decreases but the individual interneuron expression increases in adult spinal chord compared to newborn.; MISCELLANEOUS: In vitro, has no proton-gated channel activity.

Alternate Names:

anti-Amiloride-sensitive cation channel 4 pituitary antibody, anti-acid-sensing ion channel 4 antibody, anti-spinal chord ASIC antibody, anti-Spasic antibody, anti-BNAC4 antibody, anti-MGC17248 antibody, anti-MGC24860 antibody

Host:

Rabbit

Immunogen:

A synthetic peptide as a part of human, rat & mouse amiloride-sensitive cation channel 4 (ACCN4, ASIC4) conjugated to immunogenic carrier protein was used as the immunogen.

Specificity:

Appears to be specific for ASIC4.

Localization:

Cell membrane; multi-pass membrane protein

Species Reactivity:

Human, rat and mouse. Other species not yet tested.
Other species have not been tested.

Uses:

IHC, WB. A dilution of 1 : 300 to 1 : 4000 is recommended. The optimal dilution should be determined by the end user.
Other applications have not been tested.

Dilutions:

Suggested working dilutions *
immunohistochemistry
Western Blot 1:300
* Investigator should determine optimal working dilutions.

Packaging:

0.1 ml of Whole antisera antibody

Buffer:

Lyophilised in PBS

Preservative:

No Preservative

Storage:

Store at 4C for short-term. Aliquot and store at -20C for long term. Avoid freeze-thaw cycles.

Limitations:

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.

General References:

1. Gruender S, et al. NeuroReport 11:1607-1611(2000).
2. Akopian A.N, et al. NeuroReport 11:2217-2222(2000).

Notes:

Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material. Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Gene Id:

55515

Gene System:

ACCN4

Omim ID:

ASIC4_HUMAN ; ASIC4_RAT ; ASIC4_MOUSE

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