ACYP1 antibody

Goat Polyclonal anti-ACYP1

Catalog Number: NB100-57091	Price: $295.00

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Background:

Acylphosphatase is a small cytosolic enzyme that catalyzes the hydrolysis of the carboxyl-phosphate bond of acylphosphates. Two isoenzymes have been isolated, called muscle acylphosphatase and erythrocyte acylphosphatase, on the basis of their tissue localization. This gene encodes the erythrocyte acylphosphatase isoenzyme. Alternatively spliced transcript variants that encode different proteins were identified through data analysis.

Alternate Names:

anti-ACYP1 (isoform a) antibody, anti-ACYP1 (isoform a) antibody, anti-ACYP1 antibody, anti-acylphosphatase 1 antibody, anti-erythrocyte (common) typeantibody, anti-ACYPEantibody, anti-erythrocyte acylphosphatase 1 antibody, anti-ACYP1 antibody, anti-acylphosphatase 1 antibody, anti-erythrocyte (common) type antibody, anti-ACYPE antibody, anti-erythrocyte acylphosphatase 1 antibody

Host:

Goat

Immunogen:

Synthetic peptide, C-PKSHIDKANFNNEK, representing the internal region of the human protein according to NP_001098.1

Isotype:

IgG

Epitope:

PKSHIDKANFNNEK

Species Reactivity:

Reacts with Human. Expected from sequence similarity: mouse and rat
Other species have not been tested.

Homology:

Human, Mouse, Rat

Uses:

Peptide ELISA and Western Blot
In WB approx 15kDa band observed in Human Testis lysates (calculated MW of 11.3kDa according to NP_001098.1).
Other applications have not been tested.

Dilutions:

Suggested working dilutions *
ELISA 1:128000,
Western Blot 0.01-0.03ug/ml
* Investigator should determine optimal working dilutions.

Positive Controls:

* Human Testis Protein NB820-59266

Packaging:

0.1 mg Immunogen affinity purified Goat antisera.

Concentration:

0.5 mg/ml

Buffer:

Tris-saline [ pH7.3] with 0.5% BSA

Preservative:

0.02% sodium azide

Storage:

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Limitations:

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.

General References:

Paoli P, Pazzagli L, Giannoni E, Caselli A, Manao G, Camici G, Ramponi G. A nucleophilic catalysis step is involved in the hydrolysis of aryl phosphate monoesters by human CT acylphosphatase. J Biol Chem. 2003 Jan 3;278(1):194-9. Epub 2002 Oct 29. PMID: 12409302

Notes:

Other Gene ID: 66204 (mouse); This antibody is expected to recognize isoform a (NP_001098.1) only.

Gene Id:

97

Gene System:

ACYP1

NB100-57091 (0.01?g/ml) staining of Human Testes lysate (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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