ACP1 antibody

Rabbit Polyclonal anti-ACP1

Catalog Number: NB100-56474	Price: $295.00

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Background:

Official Gene Symbol: ACP1 Gen Bank Accession Number: NP_009030 Gene ID: 52 (Human) Gene Map Locus: 2p25 (Human) ACP1 (E.C 3.1.3.2) is a unique member of Phosphotyrosine Protein phosphotase family of proteins. It is ubiquitously expressed acid phosphotase functioning as an acid phosphotase and a protein tyrosine phosphotase. ACP1 has a cysteine-based catalytic mechanism and hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate. Reports suggest a functional role of ACP1 in TCR signaling and control of cell-cell adhesion through regulation of cytoskeletion assembly.

Alternate Names:

anti-Acid phosphatase 1 antibody

Host:

Rabbit

Immunogen:

A portion of amino acids 50-100 of human ACP1 was used as the immunogen.

Isotype:

IgG

Specificity:

The amino acid sequence used as immunogen is 100% homologous in human, 93% in dog and orangutan, 86% in chicken and 80% homologous in mouse and rat (isoform a). It is also 100% homologous in human ACP1 isoforms c and d. An uncharacterized 45 kD band is also seen and blocked by the immunizing peptide.

Species Reactivity:

Cross reacts with Human.
Other species have not been tested.

Uses:

Western Blot
Other applications have not been tested.

Dilutions:

Suggested working dilutions *
Western Blot 1-3 ug/ml
* Investigator should determine optimal working dilutions.

Positive Controls:

Liver lysate
* Human Liver Protein NB820-59232

Packaging:

0.1 mg protein G purified Rabbit antisera.

Buffer:

PBS containing 0.2% gelatin

Preservative:

0.05 % Sodium Azide

Storage:

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles.

Limitations:

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.

General References:

1. Wo,Y.Y., McCormack,A.L., Shabanowitz,J., Hunt,D.F., Davis,J.P., Mitchell,G.L. and Van Etten,R.L. Sequencing, cloning, and expression of human red cell-type acid phosphatase, a cytoplasmic phosphotyrosyl protein phosphatase J. Biol. Chem. 267 (15), 10856-10865 (1992)
2. Bottini et.al 277 (27) 24220-24224 (July 5, 2002)
3. Giannoni et.al JBC 278 (38) 36763-36776 (September 19, 2003)

Gene Id:

52

Gene System:

ACP1

Western blot analysis of ACP1 in the 1) absence and 2) presence of immunizing peptide in human liver lysate using NB100-56474 at 1 ug/ml.

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