ACSL5 antibody

Goat Polyclonal anti-ACSL5

Catalog Number: NB100-2918	Price: $295.00

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Background:

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in uterus and spleen, and in trace amounts in normal brain, but has markedly increased levels in malignant gliomas. This gene functions in mediating fatty acid-induced glioma cell growth. Three transcript variants encoding two different isoforms have been found for this gene.

Alternate Names:

anti-ACSL5 antibody, anti-acyl-CoA synthetase long-chain family member 5 antibody, anti-HGNC:16526 antibody, anti-ACS2 antibody, anti-ACS5 antibody, anti-FACL5 antibody, anti-fatty-acid-Coenzyme A ligase antibody, anti-long-chain 5 antibody, anti-long-chain acyl-CoA synthetase 5 antibody, anti-long-chain fatty ac antibody

Host:

Goat

Research Areas:

Cancer Research

Immunogen:

C-RTQIDSLYEHIQDC TerminusNP_057318.2; NP_976313.1; NP_976314.1

Species Reactivity:

Reacts with Human.
Other species have not been tested.

Homology:

Human

Uses:

Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 75kDa band observed in Human Spleen lysates (calculated MW of 76.0kDa according to NP_976313.1and NP_976314.1). Recommended concentration: 1-3µg/ml.
Other applications have not been tested.

Dilutions:

Suggested working dilutions *
ELISA ,
Western Blot
* Investigator should determine optimal working dilutions.

Positive Controls:

Human Spleen lysate

Packaging:

0.1 mg Immunogen affinity purified Goat antisera.

Concentration:

0.5 mg/ml

Buffer:

Tris-saline containing 0.02% Na azide [pH7.3] with 0.5% BSA

Preservative:

0.02% Sodium Azide

Storage:

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Limitations:

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.

General References:

Obermuller N, Keith M, Kopitz J, Autschbach F, Schirmacher P, Gassler N. Coeliac disease is associated with impaired expression of acyl-CoA-synthetase 5.Int J Colorectal Dis. 2005 Apr 5; [Epub ahead of print] PMID: 15809837

Notes:

This antibody is expected to recognise isoform a (NP_057318.2) and isoform b (NP_976313.1 and NP_976314.1).

Gene Id:

51703

Gene System:

ACSL5

NB 100-2918 (1?g/ml) staining of Human Spleen lysate (35?g protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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