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Rad51D Antibody

 (7 Publications)
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Product Details

Summary
Product Discontinued
View other related Rad51D Primary Antibodies

Order Details


    • Catalog Number
      NB100-166
    • Availability
      Product Discontinued

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Rad51D Antibody Summary

Immunogen
A peptide from human Rad51D (homologue 4). [UniProt# O75771]
Specificity
This is specific for hRAD51D. There is no cross-reactivity observed with XRCC2, XRCC3, RAD51, RAD51B, or RAD51C.
Clonality
Polyclonal
Host
Rabbit
Gene
RAD51D
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:1000
Application Notes
This Rad51D antibody is useful for Western blot, where it recognizes a band at 35 kDa representing Rad51D (H3 splice variant). Immunocytochemistry/Immunofluorescence and Immunoprecipitation were reported in scientific literature. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Rad51D Overexpression Lysate
Publications
Read Publications using
NB100-166 in the following applications:

  • ICC/IF
    1 publication
  • IP
    1 publication
  • WB
    4 publications

Reactivity Notes

Human. This antibody does not work in mouse.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Rad51D Antibody

  • DNA repair protein RAD51 homolog 4
  • HsTRAD
  • R51H3Trad
  • RAD51 homolog D
  • RAD51DRAD51 (S. cerevisiae)-like 3
  • RAD51-like 3 (S. cerevisiae)
  • RAD51-like protein 3
  • recombination repair protein
  • TRAD

Background

Five human RAD51 homologues have been identified: XRCC2, XRCC3, RAD51B, RAD51C, and RAD51D. Each of these homologues interacts with one or more of the other four, with all of the proteins involved in one complex or multiple smaller complexes. RAD51D interacts with XRCC2 and RAD51C. RAD51D's expression pattern and sequence similarity to other RAD51 family members most likely makes it part of a complex of proteins required for DNA repair and meiotic recombination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Rad51D Antibody (NB100-166)(7)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(1)
IP
(1)
WB
(4)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-166 Applications Species
Park JY, Virts EL, Jankowska A et al. Complementation of hypersensitivity to DNA interstrand crosslinking agents demonstrates that XRCC2 is a Fanconi anaemia gene. J. Med. Genet. 2016-05-20 [PMID: 27208205] (WB, Human) WB Human
Huang JW, Wang Y, Dhillon KK et al. Systematic Screen Identifies miRNAs That Target RAD51 and RAD51D to Enhance Chemosensitivity. Mol Cancer Res 2013-12-01 [PMID: 24088786] (WB, Human) WB Human
Chun J, Buechelmaier ES, Powell SN. Rad51 paralog complexes BCDX2 and CX3 act at different stages in the BRCA1-BRCA2-dependent homologous recombination pathway. Mol Cell Biol 2013-01-01 [PMID: 23149936] (Human) Human
Wang Y, Huang JW, Calses P et al. MiR-96 Downregulates REV1 and RAD51 to Promote Cellular Sensitivity to Cisplatin and PARP Inhibition Cancer Res 2012-08-15 [PMID: 22761336] (WB, Human) WB Human
Shammas MA, Shmookler Reis RJ, Koley H et al. Dysfunctional homologous recombination mediates genomic instability progression in myeloma. Blood 2009;113(10):2290-2297 [PMID: 19050310] (ICC/IF, Human) ICC/IF Human
Chattopadhyay R, Das S, Maiti AK et al. Regulatory role of human AP-endonuclease (APE1/Ref-1) in YB-1-mediated activation of the multidrug resistance gene MDR1. Mol Cell Biol;28(23):7066-80. 2008-12-01 [PMID: 18809583]
Wiese, C et al. Disparate requirements for the Walker A and B ATPase motifs of human RAD51D in homologous recombination. Nucleic Acids Res;34(9):2833-43. 2006-05-22 [PMID: 16717288] (WB, IP, Human) WB, IP Human

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Product General Protocols

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Video Protocols

WB Video Protocol
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Control Lysate(s)

HeLa Whole Cell Lysate
Rad51D Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RAD51D