ACTR1A antibody

Goat Polyclonal anti-ACTR1A

Catalog Number: NB100-1423	Price: $295.00

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Background:

The actin-related protein ACTR1A, a member of the superfamily of actin-related proteins, is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. The major components of the dynactin complex, consisting of at least ten polypeptides, include ACTR1A. ACTR1A, the most abundant subunit in the dynactin complex, is most similar to conventional actin (>50% amino acid identity). The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1A overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of ACTR1A/dynactin complex in progression through the prometaphase of mitosis.

Alternate Names:

anti-actin related protein 1 antibody, anti-Actin RPV antibody, anti-Alpha Centractin antibody, anti-ARP1 antibody, anti-Centrosome associated actin homolog antibody

Host:

Goat

Research Areas:

Cell Cycle and Replication, Cell Biology, Cytoskeleton

Immunogen:

Synthetic peptide: YEEDGARSIHRKT representing the C-terminus of the human protein (residues 363-375) according to NP_005727.

Isotype:

IgG

Specificity:

This antibody may crossreact with ACTR1B

Localization:

Cytoplasmic

Species Reactivity:

Reacts with human and mouse. Expected from sequence similarity: rat
Other species have not been tested.

Homology:

Mouse 100%
Rat

Uses:

ELISA and Western Blot
In Western Blot detects a band of approximately 45 kDa (predicted molecular weight 43 kDa).
Other applications have not been tested.

Dilutions:

Suggested working dilutions *
ELISA 1:8000,
Western Blot 1 - 3 ug/ml
* Investigator should determine optimal working dilutions.

Positive Controls:

* Human Brain Protein NB820-59177

Packaging:

0.1 mg Immunogen affinity purified Goat antisera.

Concentration:

0.5 mg/ml

Buffer:

Tris-saline containing 0.02% Na azide (pH7.3) with 0.5% BSA

Storage:

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Limitations:

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 6 months from date of receipt.

General References:

Lees-Miller JP et al. A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility. Nature 359:244-6 (1992). <PUBMED:1528266>

Notes:

GenBank Accession Number - NP_005727

Gene Id:

10121

Gene System:

ACTR1A

NB 100-1422 staining (1µg/ml) of human Brain lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

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