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Ku80/XRCC5 Antibody (5D5.8)

 (3 Publications)
 (1 Review)
 
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Product Details

Summary
Product Discontinued
View other related Ku80/XRCC5 Primary Antibodies

Order Details


    • Catalog Number
      NB100-103
    • Availability
      Product Discontinued

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Ku80/XRCC5 Antibody (5D5.8) Summary

Immunogen
HeLa whole cell extracts
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
XRCC5
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence 1:10-1:20
  • Simple Western 1:50
  • Western Blot 1:500
Application Notes
This Ku80 (5D5.8) antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot analysis where a band is seen at approx. 85 kDa. *The investigator should determine the optimal working dilution for a specific application.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Ku80/XRCC5 Overexpression Lysate
Reviewed Applications
Read 1 Review rated 2
using
NB100-103 in the following applications:
Publications
Read Publications using
NB100-103 in the following applications:

  • ICC/IF
    1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein G purified

Alternate Names for Ku80/XRCC5 Antibody (5D5.8)

  • 80kDa
  • ATP-dependent DNA helicase 2 subunit 2
  • ATP-dependent DNA helicase II 80 kDa subunit
  • CTC85
  • CTCBF
  • EC 3.6.4.-
  • FLJ39089
  • G22P2
  • KARP1
  • KARP-1
  • Ku80
  • Ku86 autoantigen related protein 1,86 kDa subunit of Ku antigen
  • Ku86
  • Ku86DNA repair protein XRCC5
  • KUB2
  • NFIV
  • Thyroid-lupus autoantigen
  • TLAA
  • X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining)CTC box-binding factor 85 kDa subunit
  • X-ray repair complementing defective repair in Chinese hamster cells 5(double-strand-break rejoining; Ku autoantigen, 80kD)
  • X-ray repair cross-complementing protein 5
  • X-ray repair, complementing defective, repair in Chinese hamster
  • XRCC5

Background

Heterodimeric Ku antigen (heterodimer of Ku70 and Ku80 subunits) is DNA double strand's molecular detector and it activates DNA protein kinase via direct binding in non-sequence-specific manner to free DNA termini. Encoded by XRCC5, Ku80 exist as heterodimer that associates with PRKDC in a DNA-dependent manner to form DNA-PK (DNA-dependent protein kinase) complex and binds with LIG4-XRCC4 complex also. The heterodimer also associates with NAA15 to create complex with tendency to bind DNA towards OCFRE (osteocalcin FGF response element) and also interacts with MSX2, RUNX2, ELF3, APLF, APEX1 etc. In DSBs repair, Ku80 plays its critical role in maintaining genome stability through non-homologous end joining (NHEJ) pathway mediated repair of eukaryotic DSBs which, if left unrepaired, may lead to chromosomal aberration, mutation or carcinogenesis. Ku80 involves in telomere maintenance, apoptosis, tumor suppression etc. and has been proposed to be a multifunctional caretaker with ability to suppress chromosomal aberrations and malignant transformation. Overexpression of Ku80 is associated with aggressive cancers such as bladder, cervical, pancreatic, gastric, breast and lung cancer, and is involved in drug resistance in case of ovarian cancer and leukemia. Individuals with SLE and related disorders also produce extremely large amounts of autoantibodies to Ku80.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Ku80/XRCC5
Antibody (5D5.8)
NB100-103
Ku80/XRCC5 Antibody (5D5.8)
Species: Hu
Applications: WB, Simple Western, Flow, ICC/IF

Publications for Ku80/XRCC5 Antibody (NB100-103)(3)

We have publications tested in 2 confirmed species: Human, Chinese Hamster.

We have publications tested in 3 applications: ICC/IF, IP, WB.


Filter By Application
ICC/IF
(1)
IP
(1)
WB
(2)
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Human
(2)
Chinese Hamster
(1)
All Species

Review for Ku80/XRCC5 Antibody (NB100-103) (1) 21

Average Rating: 2
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-103:
Filter by Applications
WB
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Ku80/XRCC5 NB100-103
Enlarge 		2
reviewed by:
Verified Customer
 WB Human 02/07/2014
View

Summary

ApplicationWestern Blot
Sample TestedHeLa nuclear extract on PVDF membrane
SpeciesHuman

Product General Protocols

View specific protocols for Ku80/XRCC5 Antibody (NB100-103): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Ku80/XRCC5 Antibody (NB100-103) (0)

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Control Lysate(s)

Ku80/XRCC5 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

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Calculators

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Recent Reviews

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Western Blot Ku80/XRCC5 NB100-103
Enlarge
 Verified Customer
02/07/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol XRCC5