cleaved p85 PARP Antibody (NB110-57321)
cleaved p85 PARP Antibody (Y34)
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anti-ADP ribosyltransferase NAD(+) antibody, anti-PPOL antibody, anti-Poly ADP ribosyl transferase antibody, anti-Poly ADP ribose synthetase antibody, anti-Poly ADP ribose synthetase 1 antibody, anti-Poly ADP ribose polymerase family member 1 antibody, anti-Poly ADP ribose polymerase antibody, anti-Poly ADP ribose polymerase 1 antibody, anti-PARP1 antibody, anti-PARP antibody, anti-PARP 1 antibody, anti-pADPRT1 antibody, anti-pADPRT 1 antibody, anti-NAD + ADP ribosyltransferase 1 antibody, anti-ADPRT1 antibody, anti-ADPRT antibody, anti-ADPRT 1 antibody |
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cleaved p85 PARP Antibody (Y34) Summary: | | Gene: | PARP1 |
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| Purity: | IgG purified |
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| Host: | Rabbit |
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| | Specificity: | The antibody only recognize p85 cleaved-form of PARP-1. |
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| Publications: | Antibody has at least 4 Background References. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional cleaved p85 PARP Products cleaved p85 PARP Antibody (Y34) Details: | Clone: | Y34 |
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| Isotype: | IgG |
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| Immunogen: | A synthetic peptide corresponding to residues following the cleavage site of human PARP-1 was used as immunogen. |
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Reacts in Human. Does not react to Mouse or Rat. |
| Uses: | This product is useful for: Western Blot, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 85kDa. |
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| Dilutions: | Western Blot 1:1000-10000, Immunocytochemistry 1:50, Immunoprecipitation 1:50, Flow Cytometry 1:50 |
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| TMW: | 85 kDa |
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| Unit Size: | 0.1 ml |
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| Concentration: | Please see the vial label for concentration. |
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Packaging: | Storage: | Store at -20 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.05% BSA. |
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| Preservative: | 0.01% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa and 85 kDa (2, 4).
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Images (2) Reviews (0) Related Products (3) Working with cleaved p85 PARP
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Jump to: Publications, Images, Ask a Question
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Working with cleaved p85 PARP
cleaved p85 PARP Antibody (Y34) Images (2) Flow Cytometry: PARP-1 (Cleaved p85) Antibody (Y34) [NB110-57321] - Analysis of apoptotic and non-apoptotic Jurkat cells using anti-cleaved PARP-1. Jurkat cells were either left untreated (A) or treated with camptothecin (4 uM, 5 hr) to induce apoptosis (B). Cells were fixed and permeabilized, and then stained with anti-cleaved PARP-1. The results indicate that 43% of cells were positive for cleaved PARP (B, M2) after treatment, compared to 9% positive without treatment (A, M2). |  Western Blot: PARP-1 (Cleaved p85) Antibody (Y34) [NB110-57321] - Western blot analysis on (1) Jurkat and (2) Jurkat + Camptothecin using anti-PARP-1 (catalog #110-57321), 1:1,000 dilution. |
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Publications (4)
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Related ProductsPrimary Antibodies (3)Secondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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