cleaved p25 PARP Antibody (E51) (NB110-57320)

cleaved p25 PARP Antibody (E51) (NB110-57320) (0.1 ml)

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	Catalog Number	NB110-57320
	Size(s)	0.1 ml	Price:
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anti-NAD(+) ADP-ribosyltransferase 1 antibody, anti-EC 2.4.2 antibody, anti-ADPRT 1 antibody, anti-poly(ADP-ribose) synthetase antibody, anti-PARP-1 antibody, anti-poly(ADP-ribose) polymerase antibody, anti-Poly[ADP-ribose] synthase 1 antibody, anti-poly(ADP-ribosyl)transferase antibody, anti-poly (ADP-ribose) polymerase family, member 1 antibody, anti-poly [ADP-ribose] polymerase 1 antibody, anti-ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) antibody, anti-PPOLpADPRT-1 antibody, anti-ADPRTADP-ribosyltransferase NAD(+) antibody, anti-PARPADPRT1 antibody, anti-EC 2.4.2.30 antibody, anti-poly (ADP-ribose) polymerase 1 antibody

cleaved p25 PARP Antibody (E51) Summary:

Species:	Hu, Mu	Gene:	PARP1
Applications:	WB, FACS, ICC, IHC-P, IP	Purity:	IgG purified
Clonality:	Monoclonal	Host:	Rabbit
Specificity:	The antibody only recognize p25 cleaved-form of PARP-1.
Publications:	NB110-57320 has been mentioned in at least 1 Publication

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Primary Antibodies
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cleaved p25 PARP Antibody (E51) Details:

Clone:	E51
Isotype:	IgG
Immunogen:	A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen.

Species Reactivity:

Human, Mouse. Mouse cross reactivity tested by western blot and Immunohistochemistry. Rat cross reactivity tested by Western Blot only.

Applications:

Uses:	This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 25kDa.
Dilutions:	Western Blot 1:50000-100000, Immunohistochemistry-Paraffin 1:100, Immunocytochemistry 1:250, Immunoprecipitation 1:50, Flow Cytometry 1:50
Unit Size:	0.1 ml
Concentration:	Please see the vial label for concentration.

Packaging:

Storage:	Store at -20°C. Avoid freeze-thaw cycles.
Buffer:	50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA.
Preservative:	0.01% Sodium Azide
Limitations:	This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.

Gene Symbol: PARP1

Entrez:	142 (Human)
	11545 (Mouse)
	25591 (Rat)
Uniprot:	P09874 (Human)

Related Genes:

Background:

Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa and 85 kDa (2,4).

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cleaved p25 PARP Antibody (E51) Images (3)

	Western Blot: PARP-1 (Cleaved p25) Antibody (E51) [NB110-57320] - Western blot analysis on Jurkat cell lysate using anti-PARP-1 Cleaved (p25), 1:50,000 dilution. Cells were either (1) untreated or (2) treated with Camptothecin.

	Immunofluorescent: PARP-1 (Cleaved p25) Antibody (E51) [NB110-57320] - Immunofluorescent staining of Hela cell using anit-PARP-1 (cat# 110-57320)

	Immunohistochemistry: PARP-1 (Cleaved p25) Antibody (E51) [NB110-57320] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PARP-1 (Cleaved p25) (cat. #110-57320).