cleaved p25 PARP Antibody (NB110-57320)
cleaved p25 PARP Antibody (E51)
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anti-ADP ribosyltransferase NAD(+) antibody, anti-PPOL antibody, anti-Poly ADP ribose synthetase 1 antibody, anti-Poly (ADP ribosyl) transferase antibody, anti-Poly (ADP ribose) synthetase antibody, anti-Poly (ADP ribose) synthetase 1 antibody, anti-Poly (ADP ribose) polymerase family member 1 antibody, anti-Poly (ADP ribose) polymerase antibody, anti-Poly (ADP ribose) polymerase 1 antibody, anti-PARP1 antibody, anti-PARP cleaved p25 antibody, anti-PARP antibody, anti-PARP 1 antibody, anti-pADPRT1 antibody, anti-pADPRT 1 antibody, anti-NAD(+) ADP ribosyltransferase 1 antibody, anti-ADPRT1 antibody, anti-ADPRT antibody, anti-ADPRT 1 antibody, |
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cleaved p25 PARP Antibody (E51) Summary: | | Gene: | PARP1 |
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| Purity: | IgG purified |
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| Host: | Rabbit |
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| | Specificity: | The antibody only recognize p25 cleaved-form of PARP-1. |
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| Publications: | Antibody has been mentioned in at least 1 Publication. |
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| | Note: Not all species have been tested for usefulness with this product. Only those species listed have been tested. We cannot make any guarantees about additional reactivities which may or may not occur. |
View Additional cleaved p25 PARP Products cleaved p25 PARP Antibody (E51) Details: | Clone: | E51 |
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| Isotype: | IgG |
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| Immunogen: | A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. |
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| Uses: | This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation, Flow Cytometry. In Western blot this antibody detects a band at approximately 25kDa. |
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| Dilutions: | Western Blot 1:50000-100000, Immunohistochemistry-Paraffin 1:100, Immunocytochemistry 1:250, Immunoprecipitation 1:50, Flow Cytometry 1:50 |
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| TMW: | 25 kDa |
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| Unit Size: | 0.1 ml |
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| Concentration: | Please see the vial label for concentration. |
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Packaging: | Storage: | Store at -20 °C. Avoid freeze-thaw cycles. |
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| Buffer: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
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| Preservative: | 0.01% Sodium Azide |
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| Limitations: | This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months. |
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Background: Poly (ADP-ribose) polymerase (PARP) is zinc-dependent DNA binding protein that recognizes DNA strand breaks and is presumed to play a role in DNA repair (1). As a marker for apoptosis, PARP is cleaved in vitro by many caspases, and in vivo by Caspase-3 (2,3). Existing as a 116 kDa nuclear protein, PARP is cleaved between amino acids Asp214 and Gly215 to yield two fragments of 29 kDa and 85 kDa (2,4).
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Images (3) Reviews (0) Related Products (1) Exploring cleaved p25 PARP Working with cleaved p25 PARP
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Jump to: Secondary Antibodies
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Related ProductsSecondary Antibodies| Name | Catalog# | Reactivity | Applications | Images | Publications | Reviews | |
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| Rabbit IgG Antibody [HRP] | NB730-H | Rb | ELISA, ICC, IHC-P, WB | | (5) | | | | Rabbit IgG Antibody [FITC] | NB730-F | Rb | ICC, IHC-P | | (1) | | | | Rabbit IgG Antibody [Biotin] | NB730-B | Rb | ELISA, ICC, IHC-P, WB | | | | | | Rabbit IgG, H/L Chains Antibody [DyLight 488] | NBP1-72944 | Rb | FLISA, IF, WB | (3) | | | | | Rabbit IgG Antibody [Alkaline Phosphatase] | NB730-AP | Rb | ELISA, ICC, IHC-P, WB | | (1) | | | | See All » |
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Jump to: Novus Explorer, Research Areas
| cleaved p25 PARP in Context
Research Areas related to cleaved p25 PARP (7): |
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Novus Explorer for cleaved p25 PARP gene
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![Western Blot: PARP-1 (Cleaved p25) Antibody (E51) [NB110-57320] - Western blot analysis on Jurkat cell lysate using anti-PARP-1 Cleaved (p25), 1:50,000 dilution. Cells were either (1) untreated or (2) treated with Camptothecin.](http://images.novusbio.com/images/p_57320_1.jpg "Western Blot: PARP-1 (Cleaved p25) Antibody (E51) [NB110-57320] - Western blot analysis on Jurkat cell lysate using anti-PARP-1 Cleaved (p25), 1:50,000 dilution. Cells were either (1) untreated or (2) treated with Camptothecin.")
![Immunohistochemistry: PARP-1 (Cleaved p25) Antibody (E51) [NB110-57320] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PARP-1 (Cleaved p25) (cat. #110-57320).](http://images.novusbio.com/images/p_57320_2.jpg "Immunohistochemistry: PARP-1 (Cleaved p25) Antibody (E51) [NB110-57320] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PARP-1 (Cleaved p25) (cat. #110-57320).")
![Immunofluorescent: PARP-1 (Cleaved p25) Antibody (E51) [NB110-57320] - Immunofluorescent staining of Hela cell using anit-PARP-1 (cat# 110-57320)](http://images.novusbio.com/images/p_57320_3.jpg "Immunofluorescent: PARP-1 (Cleaved p25) Antibody (E51) [NB110-57320] - Immunofluorescent staining of Hela cell using anit-PARP-1 (cat# 110-57320)")
