Novus Biologicals

Store at 4C. Do not freeze.

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Chickens were immunized with HA cleavage site (YPYDVPDYA) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. Chicken anti- human HA affinity purified antibodies were coupled to peptide immobilized on solid support.

Antibody recognizes the HA cleavage site.

Suggested working dilutions: * ELISA Coating 1:100-1:500Primary 1:1000-1:30,000 Western Blots Colorimetric detection 1:1000-1:10,000ECL 1:1000-1:30,000 Cytochemistry 1:100-1:400 *The investigator should determine the optimal working dilution for a specific application. ELISA, Western blot, and immunocytochemistry.

0.1 ml Immunogen affinity purified Chicken antisera.

0.09% Sodium Azide

anti-HA epitope tag antibody, anti-HA1 antibody, anti- HA2 antibody, anti- hemagglutinin antibody