ABCA1 Antibody
Rat Monoclonal anti-ABCA1 (5A1-1422.11), conjugated to FITC
Price: $285.00
  • Catalog Number: NB400-162
  • Unit Size: 0.1 ml
Product Images
FACS analysis of Raw (red) and Raw-cAMP (blue) cells using NB 400-162 at a dilution of 1:4,000 
Gene ID  Reference Sequence  
Species Summary Mu(+) Application Summary FACS 
Purity protein G purified Host Rat 
Clone 5A1-1422.11Conjugate FITC
Storage

Store at 4C. Do not freeze.
Background

Mutations in the ABCA1 gene (ATP-binding cassette transporter 1) have been reported in Tangier disease (TD). TD is an autosomal recessive disorder characterized by an absence of plasma HDL, cholesterol ester depositing in the reticulo-endothelial system and deviations in cellular lipid trafficking. ABCA1 mediates the apo-A1 associated export of cholesterol and phospholipids from the cell. It is expressed on the plasma membrane and the Golgi complex, and is regulated by cholesterol flux. Regulation of the cholesterol flux between HDL and macrophages is competitive between ABCA1 and SR-BI.
Research Areas

Lipid and Metabolism,     Astrocyte Markers,    

Related Diseases

Tangier Disease, Atherosclerosis, Coronary Artery Disease
Isotype

IgG2a kappa
Immunogen

Mouse ABCA1-expressing HeLa stable transfectants.

Epitope

Extracellular determinants on ABCA1 of an unknown position.
Specificity

This antibody is specific for ABCA1.
Localization

Cell Membrane
Species Reactivity

This antibody reacts with mouse ABCA1. Other species have not been tested.
Uses

NB 400-162 is not reactive in Western Blot or ELISA. The investigator should determine the optimal working dilution for a specific application.
Dilutions

flow cytometry / FACS analysis 1:1000-1/30000
Concentration

0.8 mg/ml
Packaging

0.1 ml protein G purified Rat ascites.
Buffer

Tris-glycine with 150mM NaCl
Preservative

0.05% sodium azide
Clonality

Monoclonal
Alternate Names

anti-ABC 1 antibody, anti-ABC Transporter 1 antibody, anti-ABC1 antibody, anti-ATP-binding Cassette 1 antibody, anti-ATP-binding Cassette Transporter 1 antibody, anti-CERP antibody, anti-Cholesterol Efflux Regulatory Protein antibody, anti-ABC-1 antibody
 
 
 

1. Trompier, D and Chimini, G. ABCA1, Alliance for Cellular Signaling and Nature Publishing Group, Molecular pages. doi: 10.1038/mp.a002593.01 [website: http://www.signaling-gateway.org/molecule/](2005)

2. Oram, JF and Heinecke, JW. Physiol Rev. 85:1343-72 (2005)