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- Catalog Number: NB300-528
- Unit Size: 0.2 ml
| Gene ID | 43 | Reference Sequence | |
| Species Summary | Bv(+) Hu(+) Rb(+) Fe(+) | Application Summary | ELISA, IHC, IP, IHC-Fr |
| Purity | Ascites | Host | Mouse |
| Clone | HR2 |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Background | Cholinergic neurotransmission occurs in motor, autonomic and central nervous synapses and requires very rapid inactivation of its transmitter, acetylcholine (ACh). Acetylcholinesterase (AChE) rapidly hydrolyzes ACh to acetate and choline, thereby inactivating it. AChE is found in the neuromuscular junction anchored to the basal lamina which runs between the nerve terminal and muscle membrane. AChE is also found outside the nervous and neuromuscular system in blood, lymph, germ and liver cells suggesting a role for AChE not related to cholinergic transmission. Another less specific cholinesterase, butyrylcholinesterase (BChE), seems to contribute to the regulation of the ACh concentration in the synaptic cleft. |
| Research Areas | |
| Isotype | IgG2b |
| Immunogen | Purified human cerebellar acetylcholinesterase |
| Specificity | This antibody does not detect butyrylcholinesterase (BChE). |
| Localization | Cell Membrane and Secreted |
| Species Reactivity | Cross-reacts with Human, Rabbit, Cow, Cat and Guinea pig. Not yet tested in other species. |
| Uses | Immunohistochemistry (frozen), ELISA, Immunoprecipitation. Immunohistochemical staining of AChE in human brain samples results in staining of nerve fibers and terminals. This antibody cannot be used in Western blot to detect AChE. This antibody is not suitable for FACS. |
| Dilutions |
ELISA assay dependent, immunohistochemistry 1:50, immunoprecipitation Assay dependent, Immunohistochemistry-Frozen 1:50 |
| Positive Controls: | * Antigen standard for ACHE (NM_015831) (transient overexpression lysate) |
| Packaging | 0.2 ml Mouse ascites. |
| Buffer | PBS |
| Preservative | 0.05% sodium azide |
| Clonality | Monoclonal |
| Alternate Names | anti-Acetylcholine acetylhydrolase antibody, anti-Acetylcholinesterase YT blood group antibody, anti-ACHE antibody, anti-ACHE protein antibody, anti-Apoptosis related acetylcholinesterase antibody, anti-ARACHE antibody, anti-N ACHE antibody, anti-YT antibody, anti-Yt blood group antibody |
| Gene Symbol | ACHE |
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This product has been used in:Rakonczay Z & Brimijoin S Monoclonal antibodies to human brain acetylcholinesterase: properties and applications. Cell Mol Neurobiol 8:85-93 (1988). |