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- Catalog Number: NB100-57091
- Unit Size: 0.1 mg
| Product Images | ||
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![Western Blot: ACYP1 Antibody [NB100-57091] - NB100-57091 (0.01ug/ml) staining of Human Testes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://www.novusbio.com/cart/uploads/p_57091_1.jpg)
| Gene ID | 97 | Reference Sequence | NP_001098.1 |
| Species Summary | Hu(+) | Application Summary | ELISA, WB |
| Purity | Immunogen affinity purified | Host | Goat |
| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Background | Acylphosphatase is a small cytosolic enzyme that catalyzes the hydrolysis of the carboxyl-phosphate bond of acylphosphates. Two isoenzymes have been isolated, called muscle acylphosphatase and erythrocyte acylphosphatase, on the basis of their tissue localization. This gene encodes the erythrocyte acylphosphatase isoenzyme. Alternatively spliced transcript variants that encode different proteins were identified through data analysis. |
| Isotype | IgG |
| Immunogen | Synthetic peptide, C-PKSHIDKANFNNEK, representing the internal region of the human protein according to NP_001098.1 |
| Epitope | PKSHIDKANFNNEK |
| Species Reactivity | Reacts with Human. Expected from sequence similarity: mouse and rat |
| Uses | Peptide ELISA and Western Blot In WB approx 15kDa band observed in Human Testis lysates (calculated MW of 11.3kDa according to NP_001098.1). |
| Dilutions |
ELISA 1:128000, Western Blot 0.01-0.03ug/ml |
| Concentration | 0.5 mg/ml |
| Positive Controls: | * Human Testis Protein NB820-59266 * Antigen standard for ACYP1 (NM_001107) (transient overexpression lysate) |
| Packaging | 0.1 mg Immunogen affinity purified Goat antisera. |
| Buffer | Tris-saline [ pH7.3] with 0.5% BSA |
| Preservative | 0.02% sodium azide |
| Alternate Names | anti-ACYP1 (isoform a) antibody, anti-ACYP1 (isoform a) antibody, anti-ACYP1 antibody, anti-acylphosphatase 1 antibody, anti-erythrocyte (common) typeantibody, anti-ACYPEantibody, anti-erythrocyte acylphosphatase 1 antibody, anti-ACYP1 antibody, anti-acylphosphatase 1 antibody, anti-erythrocyte (common) type antibody, anti-ACYPE antibody, anti-erythrocyte acylphosphatase 1 antibody |
| Notes |
Other Gene ID: 66204 (mouse); This antibody is expected to recognize isoform a (NP_001098.1) only. |
| Gene Symbol | ACYP1 |
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Paoli P, Pazzagli L, Giannoni E, Caselli A, Manao G, Camici G, Ramponi G. A nucleophilic catalysis step is involved in the hydrolysis of aryl phosphate monoesters by human CT acylphosphatase. J Biol Chem. 2003 Jan 3;278(1):194-9. Epub 2002 Oct 29. PMID: 12409302 |