ACTR1A Antibody
Goat Polyclonal anti-ACTR1A
Price: $295.00
  • Catalog Number: NB100-1423
  • Unit Size: 0.1 mg
Product Images
Western Blot: ARP1 homolog A Antibody [NB100-1423]- staining (2ug/ml) of human kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour.  
Gene ID 10121 Reference Sequence NP_005727. 
Species Summary Hu(+)  Application Summary ELISA, WB 
Purity Immunogen affinity purified Host Goat 
Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background

The actin-related protein ACTR1A, a member of the superfamily of actin-related proteins, is the major subunit of the dynactin complex, a key component of the cytoplasmic dynein motor machinery. The major components of the dynactin complex, consisting of at least ten polypeptides, include ACTR1A. ACTR1A, the most abundant subunit in the dynactin complex, is most similar to conventional actin (>50% amino acid identity). The dynactin complex has been shown to localize to multiple structures within the cell, including membrane organelles, the centrosome, spindle poles, and spindle pole microtubules during mitosis and prometaphase kinetochores. It regulates dyneinmediated vesicle movement on microtubules as well as spindle assembly and cell division. ACTR1A overexpression in cells results in aberrant spindle morphologies and cell cycle delay at prometaphase, suggesting a possible function of ACTR1A/dynactin complex in progression through the prometaphase of mitosis.
Isotype

IgG
Immunogen

Synthetic peptide: YEEDGARSIHRKT representing the C-terminus of the human protein (residues 363-375) according to NP_005727.

Specificity

This antibody may crossreact with ACTR1B
Localization

Cytoplasmic
Species Reactivity

Human, mouse and rat.

Uses

This antibody is useful for Peptide ELISA and Western Blot. Approx. 45kDa band observed in Human Kidney lysates (calculated MW of 42.6kDa according to NP_005727.1).

Dilutions

ELISA 1:1000, Western Blot 1-3 ug/ml
Concentration

0.5 mg/ml
Positive Controls:

* Human Brain Protein NB820-59177 * Antigen standard for ACTR1A (NM_005736) (transient overexpression lysate)
Packaging

0.1 mg Immunogen affinity purified Goat antisera.
Buffer

Tris-saline containing 0.02% Na azide (pH7.3) with 0.5% BSA
Alternate Names

anti-actin related protein 1 antibody, anti-Actin RPV antibody, anti-Alpha Centractin antibody, anti-ARP1 antibody, anti-Centrosome associated actin homolog antibody
Notes

GenBank Accession Number - NP_005727

Gene Symbol

ACTR1A
 
 
 

Lees-Miller JP et al. A vertebrate actin-related protein is a component of a multisubunit complex involved in microtubule-based vesicle motility. Nature 359:244-6 (1992).