c-Myc Antibody (9E11) [HRP] 0.1 ml



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Ordering Information: NB200-108H

  • Catalog Number
    NB200-108H
  • Displayed Format
    HRP
  • Other Formats
  • Size(s)
    0.1 ml
  • Availability
  • Price
    Shipping
  

c-Myc Antibody (9E11) [HRP] Summary

Species Human, Mouse, Chicken, Yeast
Tested Applications WB, ChIP, IHC, IHC-Fr, IHC-P, IP
Clonality
Monoclonal
Host
Mouse
Gene
MYC
Purity
Protein A purified
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c-Myc Antibody (9E11) [HRP] Details

Format
HRP
Clone
9E11
Isotype
IgG2a Kappa
Immunogen
A synthetic peptide corresponding to amino acids 408-420 (AEEQKLISEEDL) of human c-Myc, conjugated to KLH. [UniProt# P01106]
Localization
Nuclear

Species Reactivity

Human, mouse, Saccharomyces and chicken.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Chromatin Immunoprecipitation 2 ug / 500 ug extract
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 2ug/mg lysate
Application Notes
This c-Myc antibody (clone 9E11) is useful for Flow Cytometry, ChIP, Immunoprecipitation, ELISA, Immunohistochemistry- Frozen and Paraffin and Western blot.
Positive Controls
Other Available
Formats
7C Labeled NB200-108V2
Allophycocyanin Labeled NB200-108APC
Biotin Labeled NB200-108B
DyLight 350 Labeled NB200-108UV
DyLight 405 Labeled NB200-108V
DyLight 405LS Labeled NB200-108V3
DyLight 488 Labeled NB200-108G
DyLight 550 Labeled NB200-108R
DyLight 650 Labeled NB200-108C
DyLight 680 Labeled NB200-108FR
DyLight 755 Labeled NB200-108IR
FITC Labeled NB200-108F
PE Labeled NB200-108PE
PerCP Labeled NB200-108PCP
Readout System

Contact Information

Product PDFs

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Publications

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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Unit Size
0.1 ml
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Preservative
No Preservative
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol MYC
Entrez
Uniprot

Alternate Names for c-Myc Antibody (9E11) [HRP]

  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • c-Myc
  • myc proto-oncogene protein
  • myc-related translation/localization regulatory factor
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)
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Related Products by Gene

Background

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

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FAQs for c-Myc (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Bioinformatics Tool for c-Myc

Discover related pathways, diseases and genes to c-Myc. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Blogs on c-Myc

Check out the latest blog posts on c-Myc.
c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2...  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

The Myc Epitope Tags Along...
Recombinant DNA technology allows researchers to fuse epitope tags to their protein of interest and then identify that protein using tag specific antibodies. The Myc Tag can be used to purify tagged proteins by affinity chromatography or detect them b...  Read full blog post.

The c-Myc Antibody: A Major Tool in Cancer Research
C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

Use Of c-Myc Antibodies In Non-Invasive Cancer Studies
Novus Biologicals specializes in offering top quality antibody reagents to many of the transcription factor proteins that are widely expressed in cancer cells.C-Myc is one of several transcription factor proteins covered by our antibody catalog. E...  Read full blog post.