c-Myc Antibody (9E11) 0.1 ml

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Western Blot: c-Myc Antibody (9E11) [NB200-108] - Analysis of c-Myc in Jurkat whole cell lysate.
Flow Cytometry: c-Myc Antibody (9E11) [NB200-108] - c-Myc antibody was tested at 1:400 in HL-60 cells using an Alexa Fluor 488 secondary (shown in purple). M1 is defined by unstained cells.
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Ordering Information: NB200-108

  • Catalog Number
    NB200-108
  • Displayed Format
    Unconjugated
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c-Myc Antibody (9E11) Summary

Species Human, Mouse, Chicken, Yeast
Tested Applications WB, ChIP, ELISA, FLOW, IHC, IHC-Fr, IHC-P, IP
Clonality
Monoclonal
Host
Mouse
Gene
MYC
Purity
Protein A purified
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c-Myc Antibody (9E11) Details

Clone
9E11
Isotype
IgG2a Kappa
Immunogen
A synthetic peptide corresponding to amino acids 408-420 (AEEQKLISEEDL) of human c-Myc, conjugated to KLH. [UniProt# P01106]
Localization
Nuclear

Species Reactivity

Human, mouse, Saccharomyces and chicken.
Reviewed Species Read 3 rated 4.7
using NB200-108 in the following species:

Publications Read Publications using NB200-108 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Chromatin Immunoprecipitation 2 ug / 500 ug extract
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 2ug/mg lysate
Application Notes
This c-Myc antibody (clone 9E11) is useful for Flow Cytometry, ChIP, Immunoprecipitation, ELISA, Immunohistochemistry- Frozen and Paraffin and Western blot.
Positive Controls
Other Available
Formats
7C Labeled NB200-108V2
Alexa Fluor (R) 405 Labeled NB200-108AF405
Alexa Fluor (R) 488 Labeled NB200-108AF488
Alexa Fluor (R) 647 Labeled NB200-108AF647
Alexa Fluor (R) 700 Labeled NB200-108AF700
Allophycocyanin Labeled NB200-108APC
Biotin Labeled NB200-108B
DyLight 350 Labeled NB200-108UV
DyLight 405 Labeled NB200-108V
DyLight 405LS Labeled NB200-108V3
DyLight 488 Labeled NB200-108G
DyLight 550 Labeled NB200-108R
DyLight 650 Labeled NB200-108C
DyLight 680 Labeled NB200-108FR
DyLight 755 Labeled NB200-108IR
FITC Labeled NB200-108F
HRP Labeled NB200-108H
PE Labeled NB200-108PE
PerCP Labeled NB200-108PCP
Reviewed Applications
Read 3 Review rated 4.7
using
NB200-108 in the following applications:

Publications
Read Publications using
NB200-108 in the following applications:

  • 2 publications
  • 1 publication
  • 1 publication
  • 2 publications

Contact Information

Product PDFs

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Reviews

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Publications

c-Myc Antibody (NB200-108) has been mentioned in at least 8 publications.
Earn rewards if you have published using c-Myc Antibody (NB200-108).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Unit Size
0.1 ml
Concentration
1.1 mg/ml
Preservative
No Preservative
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol MYC
Entrez
Uniprot

Alternate Names for c-Myc Antibody (9E11)

  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • c-Myc
  • myc proto-oncogene protein
  • myc-related translation/localization regulatory factor
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)
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Related Products by Gene

Background

Myc genes are a family of proto-oncogenes (L- Myc, N- Myc and C- Myc) that codes for Myc proteins which are transcriptor factors implicated in cellular proliferation, differentiation, apoptosis, metabolism, adhesion and self-renovation of tumor stem cells. Myc protein can act as transcriptional activator/repressor, and is activated via response to diverse mitogenic signals (including Wnt, Shh and EGF) and has been found to be up-regulated in several types of cancers. c-Myc participates gene transcription regulation and binds DNA in a non-specific manner, yet can specifically recognizes core sequence 5'-CAC[GA]TG-3' also. c-Myc heterodimerization with another bHLH protein namely Myc-associated factor X (MAX) is required for efficient c-Myc- DNA binding. c-Myc interacts with several proteins such as TAF1C, SPAG9, PARP10, KDM5A, KDM5B, NO66, PIM2 and with FBXW7 when phosphorylated at Thr-58/Ser-62. c-Myc activate the transcription of growth-related genes and c- Myc overexpression induce cell-cycle progression thereby implicating in a variety of cancers. Moreover, a chromosomal aberration involving c-Myc has been linked to a form of B-cell chronic lymphocytic leukemia and defective c-MYC is responsible for Burkitt lymphoma also.

Reviews for c-Myc Antibody (NB200-108) (3) 4.73

Read what people are saying who have used c-Myc Antibody (NB200-108).

We have 3 reviews tested in 1 species: Human.
We have 3 reviews tested in 2: Chromatin Immunoprecipitation, Western Blot.
Average Rating: 4.7
(Based on 3 reviews)

Have you used c-Myc Antibody (NB200-108)? Submit your review and earn rewards points which can be used for merchandise & discounts.

Sort 3 reviews for c-Myc Antibody (NB200-108) by:
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Excellent
(2)
Very Good
(1)
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ChIP
(1)
WB
(2)
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Human
(3)
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Images Ratings Applications Species Reviewed By Date Details
  Very Good
ChIP Human 06/27/2012
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Summary

ApplicationChromatin Immunoprecipitation
Sample TestedHela whole nuclear lysate
SpeciesHuman

Primary Anitbody

Dilution Ratio2ug per reaction

Details

Detection NotesQ-PCR
  Excellent
WB Human 06/20/2012
Show

Summary

ApplicationWestern Blot
Sample TestedHEK293 whole cell lysate
SpeciesHuman

Blocking

Blocking Details3% Milk in PBS for 1 hour at RT

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary Manufacturer Cat#Jackson Immunoresearch
Secondary Concentration1:5000

Details

Detection NotesECL, 1 mins exposure
  Excellent
WB Human 08/10/2010
Show

Summary

ApplicationWestern Blot
Sample Tested293T Cells overexpressing myc-tagged proteins, Sample Amount: 50
SpeciesHuman
CommentsClear detection of myc-tagged fusion proteins.

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Publications for c-Myc Antibody (NB200-108) (8)

Read what people are writing who have used c-Myc Antibody (NB200-108).

We have publications tested in 3 confirmed species: Human, Rat, Yeast.

We have publications tested in 4 applications: WB, ChIP, IHC-P, IP.

Have you published using c-Myc Antibody (NB200-108)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Filter By Application
WB
(2)
ChIP
(1)
IHC-P
(1)
IP
(2)
All Applications
Filter By Species
Human
(2)
Rat
(1)
Yeast
(3)
All Species
Publication Applications Species
Munchow S, Sauter C, Jansen RP. Association of the class V myosin Myo4p with a localised messenger RNA in budding yeast depends on She proteins. J Cell Sci 112 ( Pt 10):1511-8. 1999 May. [PMID: 10212145] (IP, Yeast) IP Yeast
Pich A, Margaria E, Chiusa L. Oncogenes and male breast carcinoma: c-erbB-2 and p53 coexpression predicts a poor survival. J Clin Oncol 18(16):2948-56. 2000 Aug. [PMID: 10944127] (IHC-P, Human) IHC-P Human
Tanaka S, Diffley JF. Interdependent nuclear accumulation of budding yeast Cdt1 and Mcm2-7 during G1 phase. Nat Cell Biol4 (3):198-207. 2002 Mar. [PMID: 11836525] (IP, Yeast) IP Yeast
Zhang H et al. The Yaf9 component of the SWR1 NuA4 complexes is required for proper gene expression, histone H4 acetylation, Htz1 replacement near telomeres. Mol Cell Biol 24:9424-36. 2004 [PMID: 15485911] (ChIP, Yeast) ChIP Yeast
Robert F et al. Global position recruitment of HATs HDACs in the yeast genome. Mol Cell 16:199-209. 2004 [PMID: 15494307]
Sers JA. Gruppuso PA Nucleolar localization of hepatic c-Myc: a potential mechanism for c-Myc regulation. Biochim Biophys Acta 1743:141-50. 2005 [PMID: 15777849] (WB, Rat) WB Rat
Matsumura S et al. Activation of the Kaposi's sarcoma-associated herpesvirus major latency locus by the lytic switch protein RTA (ORF50). J Virol 79:8493-505 (2005). [PMID: 15956592] (WB, Human) WB Human
Hubbi ME, Kshitiz, Gilkes DMet al. A nontranscriptional role for HIF-1alpha as a direct inhibitor of DNA replication. Sci Signal 2013 Feb 12 [PMID: 23405012]

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

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ChIP Webinar

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FAQs for c-Myc (3)

  1. Can I use ant myc antibody for my ChIP since my protein does not have antibody yet? Am going to get a myc-tag cloned protein as transfection-ready for this assay in Caco-2 cell line. How do I start and any tips since and going to use your kit?
    • Yes, you can use c-myc antibody for your mentioned endeavors. c-Myc Antibody (9E11) (NB200-108) is one of our best sellers that has been validated for ChIP application. It has nice customer reviews and has been cited in several peer reviewed publications as well. Regarding your question on tips, can you please confirm me the catalog number of the kit you have or are planning to buy? Moreover, please let me know if you have any specific questions.
  2. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  3. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Bioinformatics Tool for c-Myc

Discover related pathways, diseases and genes to c-Myc. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Blogs on c-Myc

Check out the latest blog posts on c-Myc.
c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2...  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

The Myc Epitope Tags Along...
Recombinant DNA technology allows researchers to fuse epitope tags to their protein of interest and then identify that protein using tag specific antibodies. The Myc Tag can be used to purify tagged proteins by affinity chromatography or detect them b...  Read full blog post.

The c-Myc Antibody: A Major Tool in Cancer Research
C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

Use Of c-Myc Antibodies In Non-Invasive Cancer Studies
Novus Biologicals specializes in offering top quality antibody reagents to many of the transcription factor proteins that are widely expressed in cancer cells.C-Myc is one of several transcription factor proteins covered by our antibody catalog. E...  Read full blog post.