c-Myc Antibody (9E10) [HRP] 0.1 ml



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Ordering Information: NB600-302H

  • Catalog Number
    NB600-302H
  • Displayed Format
    HRP
  • Other Formats
  • Size(s)
    0.1 ml
  • Availability
  • Price
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c-Myc Antibody (9E10) [HRP] Summary

Species Human, Mouse, Drosophila
Tested Applications WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Monoclonal
Host
Mouse
Gene
MYC
Purity
Protein G purified
Specificity
Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
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c-Myc Antibody (9E10) [HRP] Details

Format
HRP
Clone
9E10
Isotype
IgG1 Kappa
Epitope
Leucine zipper sequence (AEEQKLISEEDLL)
Immunogen
A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc. [UniProt# P01106]

Species Reactivity

Human, mouse and Drosophila.
Reviewed Species Read 1 rated 3
using NB600-302H in the following species:

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2.0 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
This c-Myc antibody clone 9E10 is useful for Flow Cytometry (PMID: 21315712), Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Immunoprecipitation.
Positive Controls
Other Available
Formats
Allophycocyanin Labeled NB600-302APC
Biotin Labeled NB600-302B
DyLight 350 Labeled NB600-302UV
DyLight 405 Labeled NB600-302V
DyLight 405LS Labeled NB600-302V3
DyLight 488 Labeled NB600-302G
DyLight 550 Labeled NB600-302R
DyLight 650 Labeled NB600-302C
DyLight 680 Labeled NB600-302FR
DyLight 755 Labeled NB600-302IR
FITC Labeled NB600-302F
PE Labeled NB600-302PE
PerCP Labeled NB600-302PCP
Readout System
Reviewed Applications
Read 1 rated 3
using
NB600-302H in the following applications:

Contact Information

Product PDFs

Frequently Purchased Together

Reviews

Average Rating: 3
Recent Reviews
WB Tested In Human
Reviewed On: 12/18/2014
Rated: 3

c-Myc NB600-302H
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Publications

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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Unit Size
0.1 ml
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Preservative
No Preservative
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol MYC
Entrez
Uniprot

Alternate Names for c-Myc Antibody (9E10) [HRP]

  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • c-Myc
  • myc proto-oncogene protein
  • Myc
  • Myc2
  • MYCC
  • myc-related translation/localization regulatory factor
  • Niard
  • Nird
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)
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Related Products by Gene

Background

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

Reviews for c-Myc Antibody (NB600-302H) (1) 31

Read what people are saying who have used c-Myc Antibody (NB600-302H).

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1: Western Blot.
Average Rating: 3
(Based on 1 reviews)

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Images Ratings Applications Species Reviewed By Date Details
Western Blot c-Myc NB600-302H
Click to Enlarge
Average
WB Human Costache Vasilescu 12/18/2014
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Summary

ApplicationWestern Blot
Sample TestedWhole cell lysate from 293T cells, in lysis buffer
Sample Pretreatment30ug of total protein boiled in SDS buffer
SpeciesHuman

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:1000, diluted in TBST buffer+5% milk

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 1 min

Publications for c-Myc Antibody (NB600-302H) (0)

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Product General Protocols

View specific protocols for c-Myc Antibody (NB600-302H): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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FAQs for c-Myc (1)

  1. In your advice in pdf on this product you state: recognizes the c-myc peptide in random coil configuration, not as a helix. Does this refer to the configuration of the native c-myc protein in the tissue or to the synthesized peptide sequence AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA in vitro, or to both?
    • c-Myc contains a helix-loop-helix motif in its native form. This antibody is meant to detect the common c-myc epitope tag, not the native protein.

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Bioinformatics Tool for c-Myc

Discover related pathways, diseases and genes to c-Myc. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool
Vizit™, under license from BioVista Inc.

Blogs on c-Myc

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The Myc Epitope Tags Along...
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Use Of c-Myc Antibodies In Non-Invasive Cancer Studies
Novus Biologicals specializes in offering top quality antibody reagents to many of the transcription factor proteins that are widely expressed in cancer cells.C-Myc is one of several transcription factor proteins covered by our antibody catalog. Enc...  Read full blog post.