c-Myc Antibody (9E10) [DyLight 650] 0.1 ml

Click image to view larger
Western Blot: c-Myc Antibody (9E10) [DyLight 650] [NB600-302C] - Analysis of c-myc in Jurkat cell lysates
Download Datasheet
See All Related

Ordering Information: NB600-302C

  • Catalog Number
    NB600-302C
  • Displayed Format
    DyLight 650
  • Other Formats
  • Size(s)
    0.1 ml
  • Availability
  • Price
    Shipping
  

c-Myc Antibody (9E10) [DyLight 650] Summary

Species Human, Mouse, Drosophila
Tested Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Monoclonal
Host
Mouse
Gene
MYC
Purity
Protein G purified
Specificity
Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
Guarantee Plus
All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

c-Myc Antibody (9E10) [DyLight 650] Details

Format
DyLight 650 (A=652, E=672)
Clone
9E10
Isotype
IgG1 Kappa
Epitope
Leucine zipper sequence (AEEQKLISEEDLL)
Immunogen
A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc. [UniProt# P01106]

Species Reactivity

Human, mouse and Drosophila.
Publications Read Publications using NB600-302C in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 0.5-2.0 ug/ml
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
This c-Myc antibody clone 9E10 is useful for Flow Cytometry (PMID: 21315712), Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Immunoprecipitation.
Positive Controls
Other Available
Formats
7C Labeled NB600-302V2
Allophycocyanin Labeled NB600-302APC
Biotin Labeled NB600-302B
DyLight 350 Labeled NB600-302UV
DyLight 405 Labeled NB600-302V
DyLight 405LS Labeled NB600-302V3
DyLight 488 Labeled NB600-302G
DyLight 550 Labeled NB600-302R
DyLight 680 Labeled NB600-302FR
DyLight 755 Labeled NB600-302IR
FITC Labeled NB600-302F
HRP Labeled NB600-302H
PE Labeled NB600-302PE
PerCP Labeled NB600-302PCP
Publications
Read a Publication using
NB600-302C in the following applications:

  • 1 publication

Contact Information

Product PDFs

Frequently Purchased Together

Reviews

Earn rewards points by submitting your review for c-Myc Antibody (NB600-302C). Be the first to review this product and earn double rewards points!

Publications

c-Myc Antibody (NB600-302C) has been mentioned in at least 1 publication.
Earn rewards if you have published using c-Myc Antibody (NB600-302C).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50 mM Sodium Borate
Unit Size
0.1 ml
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Preservative
No Preservative
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol MYC
Entrez
Uniprot

Alternate Names for c-Myc Antibody (9E10) [DyLight 650]

  • Myc
  • Myc2
  • Niard
  • Nird
  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • c-Myc
  • myc proto-oncogene protein
  • myc-related translation/localization regulatory factor
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)
Show More

Related Products by Gene

Background

The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.

Reviews for c-Myc Antibody (NB600-302C) (0)

There are no reviews for c-Myc Antibody (NB600-302C).
By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image
Have you used c-Myc Antibody (NB600-302C)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Points can be redeemed for discounts and gift cards:
$50/€35/£30/$50CAN Amazon Gift Card1000 Points
Items ships for Free, please disregard any shipping charges.
$50/€35/£30/$50CAN Off Purchase (credited after checkout)1000 Points
Discount is removed from total when the order is processed. The discount will not appear in your shopping cart on check out.

Publications for c-Myc Antibody (NB600-302C) (1)

Read what people are writing who have used c-Myc Antibody (NB600-302C).

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 applications: WB.

Have you published using c-Myc Antibody (NB600-302C)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Filter By Application
WB
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Product General Protocols

View specific protocols for c-Myc Antibody (NB600-302C): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

Ask a Scientist

Submit your question on below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.

FAQs for c-Myc (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Customers Who Viewed This Item Also Viewed...

NB200-103
Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications WB, ELISA, Flow...MORE

     2 Reviews

18 Publications
NB110-75039
Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NB110-75039] - Rat trigeminal using Rabbit antibody to cFos: whole serum at a 1:500 dilution.Immunohistochemistry-Paraffin: c-Fos Antibody [NB110-75039] - IHC on rat spinal cord (paraffin section) using Rabbit antibody to cFos (c-fos): whole serum at 1: 300 dilution incubated overnight at 4C and developed with ABC/DAB/Ni.Immunocytochemistry/Immunofluorescence: c-Fos Antibody [NB110-75039] - Rabbit antibody to cFos (c-fos): whole serum (NB110-75039) at a 1 : 500 dilution and DAPI counterstained appearing in blue.

Rabbit Polyclonal
Species Mouse, Rat
Applications WB, ICC/IF, IHC...MORE

NBP2-29463
Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - Formalin-fixed, paraffin-embedded human urinary transitional cell carcinoma stained with p21 antibody at 1:50 using peroxidase-conjugate and DAB chromogen.

Mouse Monoclonal
Species Human, Mouse, Rat...MORE
Applications WB, ELISA, Flow...MORE

8 Publications
NB110-55551
Western Blot: Bcl-2 Antibody (E17) [NB110-55551] -  Jurkat cell lysate.Immunohistochemistry: Bcl-2 Antibody (E17) [NB110-55551] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-BCL-2 Rab MabFlow Cytometry: Bcl-2 Antibody (E17) [NB110-55551] - Analysis of permeabilized Jurkat cells.

Rabbit Monoclonal
Species Human
Applications WB, Flow, ICC/IF...MORE

14 Publications
NB100-79920
Western Blot: Cyclin D1 Antibody (EPR2241) [NB100-79920] - Lane 1 : Mouse kidney. Lane 2 : Mouse spleen. Lane 3 : Rat heart.Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody (EPR2241) [NB100-79920] - MCF7 cells.Immunohistochemistry-Paraffin: Cyclin D1 Antibody (EPR2241) [NB100-79920] - Staining of paraffin embedded human endometrial adenocarcinoma. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC...MORE

     1 Review

1 Publication
NB110-55569
Western Blot: c-jun Antibody (E254) [NB110-55569] - NIH3T3 cell lysate using a 1:2,000 dilution.Immunocytochemistry/Immunofluorescence: c-jun Antibody (E254) [NB110-55569] - Staining c-Jun in HeLa cells.Immunohistochemistry: c-jun Antibody (E254) [NB110-55569] - Analysis of paraffin-embedded skin carcinoma using anti-c-Jun (N-term)

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF...MORE

     1 Review

2 Publications
NB200-109
Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Western blot analysis of n-Myc expression in IMR32 whole cell lysate.Immunocytochemistry/ Immunofluorescence: n-Myc Antibody (NMYC-1) [NB200-109] - The n-Myc (NMYC-1) antibody was tested in NTERA2 cells at a 1:50 dilution against Dylight 488 (Green). Actin and nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Western blot analysis of n-Myc expression in human heart tissue.

Mouse Monoclonal
Species Human, Mouse
Applications WB, ChIP, GS...MORE

2 Publications
NB200-106
Western Blot: p19ARF Antibody [NB200-106] - WB analysis of p19ARF in 1. NIH/3T3 and B. MEF cell lysate.Immunocytochemistry/Immunofluorescence: p19ARF Antibody [NB200-106] - p19ARF antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Rabbit Polyclonal
Species Mouse
Applications WB, ELISA, ICC/IF...MORE

39 Publications
AF4304
Western blot shows lysates of LNCaP human prostate cancer cell line and PC-3 human prostate cancer cell line. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human Max Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4304) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

Goat Polyclonal
Species Human
Applications WB

AF1095
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated<BR>(-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human Phospho-EGF R/ErbB1 (Y1173) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # <A class=NoLineLink href=Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human EGF (Catalog # <A class=NoLineLink href=A431 human epithelial carcinoma cells were untreated (open histogram), or treated for 5 minutes with 100 ng/mL Recombinant Human EGF (Catalog # <A class=NoLineLink href=

Rabbit Polyclonal
Species Human
Applications WB, Simple Western, IHC...MORE

2 Publications
NB100-2379
Western Blot: OCT4 Antibody [NB100-2379] - Detection of OCT4 in mouse brain lysate.Immunocytochemistry/Immunofluorescence: OCT4 Antibody [NB100-2379] - OCT4 staining in NTERA-2 cells detected with a Dylight 488 labeled secondary antibody.Flow Cytometry: OCT4 Antibody [NB100-2379] - Staining of NTERA-2  cells using NB100-2379 at a 1:50 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody.

Rabbit Polyclonal
Species Human, Mouse, Rat...MORE
Applications WB, Flow, ICC/IF

5 Publications
NB110-57027
Western Blot: vH RAS Antibody (Y132) [NB110-57027] A: MCF7 B: PC12 cell lysate using a 1:500 dilution.Immunocytochemistry/Immunofluorescence: vH RAS Antibody (Y132) [NB110-57027] - Immunocytochemical staining of MCF7 cells using anti-H-Ras (C-term) (catalog #110-57027).Flow Cytometry: vH RAS Antibody (Y132) [NB110-57027] - Overlay histogram showing HeLa cells stained with Anti-GTPase HRAS antibody [Y132]  (red line).

Rabbit Monoclonal
Species Human, Mouse, Rat
Applications WB, Flow, ICC/IF...MORE

3 Publications
NBP2-31674
Immunohistochemistry: TCEAL1 Antibody [NBP2-31674] - liver cancerImmunohistochemistry: TCEAL1 Antibody [NBP2-31674] - Immunohistochemical staining of human ovary shows strong cytoplasmic positivity in follicle cells.Immunohistochemistry: TCEAL1 Antibody [NBP2-31674] - ovary

Rabbit Polyclonal
Species Human
Applications IHC

NBP1-87425
Western Blot: D4S234E Antibody [NBP1-87425] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)<br/>Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)Immunocytochemistry/Immunofluorescence: D4S234E Antibody [NBP1-87425] - Staining of human cell line A-431 shows positivity in mitochondria.Immunohistochemistry-Paraffin: D4S234E Antibody [NBP1-87425] - Immunohistochemical staining of human bone marrow shows strong nuclear positivity in bone marrow poietic cells.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, ICC/IF, IHC...MORE

NBP1-19806
Western Blot: EGF Antibody [NBP1-19806] - Western blot (WB) analysis of EGF (R709) pAb in extracts from NIH-3T3 cells.Immunohistochemistry-Paraffin: EGF Antibody [NBP1-19806] - IHC staining of cells surrounding a follicle and the cytoplasm of the follicle using anti-EGF antibody.Immunohistochemistry: EGF Antibody [NBP1-19806] - Human brain tissue.

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-P

3 Publications
NBP1-49793
Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (SP3) [NBP1-49793] - IHC staining at 1:50 on HER+ human breast carcinoma, using a citrate antigen-retrieval buffer. Image from verified customer review.Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (SP3) [NBP1-49793] - c-erbB-2/HER-2Immunohistochemistry-Paraffin: ErbB2/HER2 Antibody (SP3) [NBP1-49793] Human Breast Carcinoma

Rabbit Monoclonal
Species Human, Mouse (Negative)
Applications IHC, IHC-P

     1 Review

NB120-3077
Western Blot: RB1 Antibody (1F8-Rb1) [NB120-3077] - Analysis of RB1 was performed by loading 25 ug of K562 (Lane 1), B-3 (Lane 2) and rat eye (Lane 3) cell lysates onto an SDS polyacrylamide gel.Immunocytochemistry/Immunofluorescence: RB1 Antibody (1F8-Rb1) [NB120-3077] - Analysis of RB1 (green) showing staining in the nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left).Immunohistochemistry-Paraffin: RB1 Antibody (1F8-Rb1) [NB120-3077] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with RB1 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.

Mouse Monoclonal
Species Human, Mouse
Applications WB, Flow, ICC/IF...MORE

2 Publications



NB100-56104
Western Blot: Bcl-Xl Antibody [NB100-56104] - Analysis of Bcl-X in lysates from isolated rat liver mitochondria using this antibody. The antibody detected Bcl-XL at ~30 kDa.Immunohistochemistry-Paraffin: Bcl-Xl Antibody [NB100-56104] - Formalin-fixed paraffin-embedded sections of human epidermis stained for Bcl-X expression using this antibody at the bottom of the images were negative for Bcl-X expression. The adjacent stratum spinosum and middle stratum granulosum layers were both very positive for Bcl-X expression. The top stratum corneum consisting mainly of dead or peeling cells were both negative for Bcl-X expression.Western Blot: Bcl-Xl Antibody [NB100-56104] - Analysis of Bcl-X in human breast carcinoma using this antibody at 1:2000. A. Formalin-fixed, paraffin-embedded tissue section from invasive ductal carcinoma. An organellar/mitochondrial pattern of Bcl-X staining is observed in the cytosol of tumor cells. B. analysis of tissue lysates from breast surgical tumor tissue biopsies (Lanes 1-7). Bcl-XS is not detected and variable amounts of Bcl-XL (~30 kDa) is is detected. Lane 8. Recombinant Bcl-XL (positive control).

Rabbit Polyclonal
Species Human, Mouse, Rat
Applications WB, IHC, IHC-Fr...MORE

1 Publication

Bioinformatics Tool for c-Myc

Discover related pathways, diseases and genes to c-Myc. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Blogs on c-Myc

Check out the latest blog posts on c-Myc.
c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2...  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

The Myc Epitope Tags Along...
Recombinant DNA technology allows researchers to fuse epitope tags to their protein of interest and then identify that protein using tag specific antibodies. The Myc Tag can be used to purify tagged proteins by affinity chromatography or detect them b...  Read full blog post.

The c-Myc Antibody: A Major Tool in Cancer Research
C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

Use Of c-Myc Antibodies In Non-Invasive Cancer Studies
Novus Biologicals specializes in offering top quality antibody reagents to many of the transcription factor proteins that are widely expressed in cancer cells.C-Myc is one of several transcription factor proteins covered by our antibody catalog. E...  Read full blog post.