Jump to Information on c-Myc Antibody (NB600-302B)
c-Myc Antibody (9E10) [Biotin] Summary
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Species
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Human, Mouse, Drosophila |
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Tested Applications
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WB, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P |
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Clonality
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Monoclonal |
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Host
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Mouse |
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Gene
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MYC |
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Purity
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IgG purified |
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Specificity
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The recognizes the c-myc peptide in random coil configuration, not as a helix. 9E10 does not react with V-myc. |
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Guarantee Plus
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All of our products are backed by our 100% guarantee to work for stated and predicted species and applications. |
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Innovator's Reward
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Test in a species/application not listed above to receive a full credit towards a future purchase.
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c-Myc Antibody (9E10) [Biotin] Details
| Conjugate |
Biotin |
| Clone |
9E10 |
| Isotype |
IgG1 Kappa |
| Marker |
c-Myc Tag |
| Epitope |
Leucine zipper sequence (AEEQKLISEEDLL) |
| Immunogen |
Synthetic peptide CT32 (myc) sequence: AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA |
Applications/Dilutions
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Dilutions
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- Western Blot 0.5-2.0 ug/ml
- Flow Cytometry 1:50-1:200
- Immunocytochemistry/Immunofluorescence 1:50-1:200
- Immunohistochemistry 1:50-1:200
- Immunohistochemistry-Frozen 1:50-1:200
- Immunohistochemistry-Paraffin 1:50-1:200
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Application Notes
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This antibody has been tested in Western blot, Immunoprecipitation, ELISA, Immunohistochemistry (Formalin-fixed paraffin-embedded, acetone-fixed, and frozen sections), Immunofluorescence, and Flow Cytometry. |
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Positive Controls
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3 Positive Controls |
Directly Conjugated
Formats
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Learn about Custom Antibody Labeling
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Product PDFs
Reviews
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Publications
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Protocols and FAQs
Controls & Support Products
Research Tools
Packaging, Storage & Formulations
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Storage
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Store at 4C in the dark. |
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Buffer
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PBS |
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Unit Size
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0.1ml |
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Concentration
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1.0 mg/ml |
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Preservative
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No Preservative |
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Limitations
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This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt. |
Bioinformatics
Alternate Names for c-Myc
- avian myelocytomatosis viral oncogene homolog slid
- BHLHE39
- bHLHe39MRTL
- c-Myc
- Class E basic helix-loop-helix protein 39
- myc proto-oncogene protein
- myc-related translation/localization regulatory fa
- Proto-oncogene c-Myc
- Transcription factor p64
- v-myc avian myelocytomatosis viral oncogene homolo
- v-myc myelocytomatosis viral oncogene homolog (avi
Related Products by Gene
TP53,
FOS,
CDKN1A,
BCL2,
CCND1,
JUN,
RAS,
MYCN,
CDKN2A,
MAX,
EGFR,
POU5F1,
HRAS,
TCEAL1,
NSG1,
EGF,
ERBB2,
RB1,
TNF,
BCL2L1
Background
The c-Myc protein is a transcription factor, which is encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin dependent kinase, ERK2 and C-Jun N terminal Kinase (JNK) in cell proliferation and cell cycle regulation. Studies also have shown that c-Myc is essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells, and which brought extensive attention in the development of new therapeutic approach for cancer treatment.
Images for c-Myc (1)
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Product Specific and General Protocols
View specific protocols for c-Myc:
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
ICC/IF Video Protocol
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WB Video Protocol
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Ask a Scientist
Submit your question on NB600-302B below.
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FAQs for c-Myc (0)
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Bioinformatics Tool for c-Myc
Discover related pathways, diseases and genes to c-Myc. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Diseases for c-Myc
Discover more about diseases related to c-Myc.
- Neoplasms
- Malignant Neoplasms
- Cell Transformation, Neoplastic
- Carcinoma
- Lymphoma
- Leukemia
- Malignant Paraganglionic Neoplasm
- Malignant Neoplasm Of Breast
- Mammary Neoplasms
- Burkitt Lymphoma
- Carcinogenesis
- Chromosomal Translocation
- Neoplasm Metastasis
- Hyperplasia
- B-cell Lymphomas
- Adenocarcinoma
- Liver Carcinoma
- Retinoblastoma
- Lung Neoplasms
- Myeloid Leukemia
Pathways for c-Myc
View related products by pathway.
- Cell Cycle
- Cell Proliferation
- Cell Growth
- Cell Death
- Pathogenesis
- Localization
- Translation
- Cell Differentiation
- S Phase
- Cell Cycle Arrest
- Oncogenesis
- Methylation
- Induction Of Apoptosis
- G1 Phase
- Senescence
- Angiogenesis
- Programmed Cell Death
- Dna Replication
- Secretion
- Regeneration
Research Areas for c-Myc
Find related products by research area.
PTMs for c-Myc
Learn more about PTMs related to c-Myc.
- Phosphorylation
- Methylation
- Cleavage
- Acetylation
- Ubiquitination
- Dephosphorylation
- Reduction
- Phosphorothioation
- Demethylation
- Deacetylation
- Glycosylation
- Biotinylation
- Oxidation
- Deamination
- Sumoylation
- Ribosylation
- Farnesylation
- Prenylation
- Palmitoylation
- Amidation
Blogs on c-Myc
Check out the latest blog posts on c-Myc.
![Western Blot: c-Myc Antibody (9E10) [Biotin] [NB600-302B] - Analysis of c-myc in Jurkat cell lysates using NB600-302.](http://images.novusbio.com/fullsize2/c-Myc Antibody (9E10) [Biotin]-Western Blot-NB600-302B-img0003.jpg)
-Western-Blot-NB200-103-img0001.jpg "Western Blot: p53 Antibody (PAb 240) [NB200-103] - WB analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.")
-Western-Blot-NB500-303-img0004.jpg "Western Blot: p21 Antibody (WA-1) [NB500-303] - WB analysis of p21 in horse skin cells. Image courtesy of prodcut review submitted by Karine Deschene.")
-Immunocytochemistry-Immunofluorescence-NB500-303-img0003.jpg "Immunocytochemistry/Immunofluorescence: p21 Antibody (WA-1) [NB500-303] - IF analysis of p21 in horse skin cells. Image courtesy of prodcut review submitted by Karine Deschene.")
-Western Blot-NB110-55551-img0004.jpg "Western Blot: Bcl2 Antibody (E17) [NB110-55551] Jurkat cell lysate.")
-Immunohistochemistry-NB110-55551-img0002.jpg "Immunohistochemistry: Bcl2 Antibody (E17) [NB110-55551] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-BCL-2 Rab Mab")
-Western Blot-NB110-56901-img0004.jpg "Western Blot: Cyclin D1 Antibody (EP272Y) [NB110-56901] - MCF-7 cell lysate.")
-Immunocytochemistry-Immunofluorescence-NB110-56901-img0005.jpg "Immunocytochemistry/Immunofluorescence: Cyclin D1 Antibody (EP272Y) [NB110-56901] MCF-7 cells.")
-Western Blot-NB110-55569-img0003.jpg "Western Blot: c-jun Antibody (E254) [NB110-55569] NIH3T3 cell lysate using a 1:2,000 dilution.")
-Immunohistochemistry-NB110-55569-img0002.jpg "Immunohistochemistry: c-jun Antibody (E254) [NB110-55569] - Immunohistochemical analysis of paraffin-embedded skin carcinoma using anti-c-Jun (N-term)")
-Western-Blot-NB200-109-img0002.jpg "Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Western blot analysis of n-Myc expression in IMR32 whole cell lysate.")
-Western-Blot-NB200-109-img0001.jpg "Western Blot: n-Myc Antibody (NMYC-1) [NB200-109] - Western blot analysis of n-Myc expression in human heart tissue.")
-Western Blot-NB110-56948-img0004.jpg "Western Blot: EGFR [p Tyr1173] Antibody (E124) [NB110-56948] - (1) A431 and (2) A431 + EGF cell lysates, 1:1,000 dilution.")
-Immunocytochemistry-Immunofluorescence-NB110-56948-img0005.jpg "Immunocytochemistry/Immunofluorescence: EGFR [p Tyr1173] Antibody (E124) [NB110-56948] Phosphatase-treated A431 cells using Phospho-EGFR.")
-Western Blot-NB110-57027-img0003.jpg "Western Blot: vH RAS Antibody (Y132) [NB110-57027] A: MCF7 B: PC12 cell lysate using a 1:500 dilution.")
-Immunocytochemistry-Immunofluorescence-NB110-57027-img0004.jpg "Immunocytochemistry/Immunofluorescence: vH RAS Antibody (Y132) [NB110-57027] - Immunocytochemical staining of MCF7 cells using anti-H-Ras (C-term) (catalog #110-57027).")
-Western Blot-NBP2-01201-img0002.jpg "Western Blot: ErbB2/HER2 Antibody (3F11) [NBP2-01201] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ErbB2/HER2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ErbB2/HER2.")
-Immunocytochemistry-Immunofluorescence-NBP2-01201-img0009.jpg "Immunocytochemistry/Immunofluorescence: ErbB2/HER2 Antibody (3F11) [NBP2-01201] Staining of COS7 cells transiently transfected by pCMV6-ENTRY ErbB2/HER2.")
-Western-Blot-NB120-3077-img0004.jpg "Western Blot: Retinoblastoma 1 Antibody (1F8-Rb1) [NB120-3077] - Human Cells-17.")
-Immunohistochemistry-Paraffin-NB120-3077-img0001.jpg "Immunohistochemistry-Paraffin: Retinoblastoma 1 Antibody (1F8-Rb1) [NB120-3077] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with Retinoblastoma (Rb) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.")
The autoantigen p90 i...