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c-Myc Antibody (9E10) (NB600-302) (0.1 ml)

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Catalog NumberNB600-302 ( Sample Size Available )
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View All c-Myc Primary Antibodies
(see alternate names)
anti-BHLHE39 antibody, anti-Proto-oncogene c-Myc antibody, anti-myc-related translation/localization regulatory factor antibody, anti-Class E basic helix-loop-helix protein 39 antibody, anti-myc proto-oncogene protein antibody, anti-v-myc avian myelocytomatosis viral oncogene homolog antibody, anti-avian myelocytomatosis viral oncogene homolog antibody, anti-c-Myc antibody, anti-bHLHe39MRTL antibody, anti-Transcription factor p64 antibody, anti-v-myc myelocytomatosis viral oncogene homolog (avian) antibody
c-Myc Antibody (9E10) Summary:
Species: Hu, Mu, Dr Gene: MYC
Applications: WB, ELISA, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP Purity: Protein G purified
Clonality: Monoclonal Host: Mouse
Specificity: Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
Publications: NB600-302 has been mentioned in at least 14 Publications
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Peptides and Proteins Primary Antibodies
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c-Myc Antibody (9E10) Details:
Clone: 9E10
Isotype: IgG1 Kappa
Immunogen: A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRN SCA) of human c-Myc. [UniProt# P01106]
Marker: c-Myc Tag
Epitope: Leucine zipper sequence (AEEQKLISEEDLL)
  Species Reactivity:
Human, mouse and Drosophila.
  Applications:
Uses: This c-Myc antibody clone 9E10 is useful for Flow Cytometry (PMID: 21315712), Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Immunoprecipitation.
Dilutions: ELISA 1:100-1:2000
Flow Cytometry 1:50-1:200
Immunocytochemistry/Immunofluorescence 1:50-1:200
Immunohistochemistry 1:50-1:200
Immunohistochemistry-Frozen 1:50-1:200
Immunohistochemistry-Paraffin 1:50-1:200
Immunoprecipitation 1:10-1:500
Western Blot 0.5-2.0 ug/ml
Unit Size: 0.1 ml (also available: 0.025 ml (sample size) )
Concentration: 1 mg/ml
Positive Controls: 2 Positive Controls
Conjugated Antibodies: 5 Conjugated Antibodies
Packaging:
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Buffer: Tris-glycine, 150 mM NaCl
Preservative: 0.05% Sodium Azide
Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis. Products are guaranteed for 6 months from date of receipt, except for peptides and proteins which are guaranteed for 3 months.
 
Gene Symbol: MYC
Entrez 4609 (Human)
Uniprot P01106 (Human)
Related Genes
Background:

Myc genes are a family of proto-oncogenes (L- Myc, N- Myc and C- Myc) that codes for Myc proteins which are transcriptor factors implicated in cellular proliferation, differentiation, apoptosis, metabolism, adhesion and self-renovation of tumor stem cells. Myc protein can act as transcriptional activator/repressor, and is activated via response to diverse mitogenic signals (including Wnt, Shh and EGF) and has been found to be up-regulated in several types of cancers. c-Myc participates gene transcription regulation and binds DNA in a non-specific manner, yet can specifically recognizes core sequence 5'-CAC[GA]TG-3' also. c-Myc heterodimerization with another bHLH protein namely Myc-associated factor X (MAX) is required for efficient c-Myc- DNA binding. c-Myc interacts with several proteins such as TAF1C, SPAG9, PARP10, KDM5A, KDM5B, NO66, PIM2 and with FBXW7 when phosphorylated at Thr-58/Ser-62. c-Myc activate the transcription of growth-related genes and c- Myc overexpression induce cell-cycle progression thereby implicating in a variety of cancers. Moreover, a chromosomal aberration involving c-Myc has been linked to a form of B-cell chronic lymphocytic leukemia and defective c-MYC is responsible for Burkitt lymphoma also.

Images (2)
Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302. Immunocytochemistry/Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells stained NB600-302 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).
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Reviews (3)
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Ju-Pi Li on 11/30/2011
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Related Products (695)
2 Positive Controls
5 Conjugated Antibodies
1 Antibody Pairs
1 Lysates
3 Peptides and Proteins
65 Primary Antibodies
1 RNAi
Secondary Antibodies
 
Exploring c-Myc
10   Related Research Areas
 
Working with c-Myc
14   Publications
3   Reviews from researchers like you
1   Protocols and Procedures
Jump to: Publications, Images, Reviews, Protocols/Procedures, Ask a Question
Working with c-Myc  

c-Myc Antibody (9E10) Images (2)

Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.
 

(view larger) 		Immunocytochemistry/Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells stained NB600-302 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).
 
 

Reviews (3)  Have you used this product? Review this product to earn Novus Rewards.

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Reviewed by:
Application: Western Blot
Sample Tested: Jurkat T-Cells
Sample Pretreatment: RZPA Lysis
Species: Human
Blocking Details: 5% non-fat milk
Dilution Ratio: 1:1000
Secondary Description: sigma anti-mouse
Detection Notes: ECL
 
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Reviewed by:
Application: Western Blot
Sample Tested: HeLa cell lysate
Sample Pretreatment: 10 µl
Sample Pretreatment: RIPA buffer
Species: Human
Blocking Details: 5% Milk in PBST
Dilution Ratio: 1:500
Secondary Description: anti-mouse IgG
Secondary Manufacturer Cat#: NA9320
Detection Notes: ECL plus
 
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Reviewed by:
Application: Western Blot
Sample Tested: 293T Cell Lysates overexpressing myc-tagged fusion proteins
Sample Pretreatment: 50 ug
Species: Human
Blocking Details: 5% Milk in PBST
Dilution Ratio: 1:1000
 

Publications (14)

 
c-Myc Antibody (9E10) Product Specific:
  1. Wang H, Shi LZ, Wong CC et al. The Interaction of CtIP and Nbs1 Connects CDK and ATM to Regulate HR–Mediated Double-Strand Break Repair. PLoS Genet 2013 Feb [PMID: 23468639] (WB)
  2. Lu CS, Truong LN, Aslanian A et al The RING finger protein RNF8 ubiquitinates Nbs1 to promote DNA double-strand break repair by homologous recombination J Biol Chem 2012 Oct 31 [PMID: 23115235] (IP)

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  1. Santini E, Feyder M, Gangarossa G et al Dopamine- and cAMP-regulated Phosphoprotein of 32-kDa (DARPP-32)-dependent Activation of Extracellular Signal-regulated Kinase (ERK) and Mammalian Target of Rapamycin Complex 1 (mTORC1) Signaling in Experimental Parkinsonism J Biol Chem 2012 Aug 10 [PMID: 22753408] (IP, Mouse)
  2. Hubbi ME, Luo W, Baek JH, et al. MCM proteins are negative regulators of hypoxia-inducible factor 1. Mol Cell. Jun. 2011 [PMID: 21658608] (IP, WB, Human)
  3. Wang H, Shao Z, Shi LZ et al. CtIP Protein Dimerization Is Critical for Its Recruitment to Chromosomal DNA Double-stranded Breaks J Biol Chem 2012 Jun 15 [PMID: 22544744] (IP, WB, Human)
  4. Zhao Y, Wang Y, Hu J, Zhang X, Zhang YW CutA divalent cation tolerance homolog (E. coli) (CUTA) regulates Beta-cleavage of Beta-amyloid precursor protein (APP) through interacting with Beta-Site APP cleaving protein 1 (BACE1) J Biol Chem 2012 Feb 17 [PMID: 22351782] (WB, Human)
  5. Trinkle-Mulcahy L, Chusainow J, Lam YW, Swift S, Lamond A. Visualization of intracellular PP1 targeting through transiently and stably expressed fluorescent protein fusions. Methods Mol Biol;365:133-54. 2007. [PMID: 17200560]
  6. Bergman, M., et al. Cardiac matrix metalloproteinase-2 expression independently induces marked ventricular remodeling and systolic dysfunction. Am J Physiol Heart Circ Physiol;292(4):H1847-60. 2007 Apr. [PMID: 17158653]
  7. Hilpert K, Hansen G, Wessner H, Küttner G, Welfle K, Seifert M, Höhne W. Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. Protein Eng;14(10):803-6. 2001 Oct. [PMID: 11739900]
  8. Kari B et al. Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes. J Virol;60(2):345-52. 1986 Nov. [PMID: 3021969]
  9. Murri-Plesko MT, Hulikova A, Oosterwijk E, et al. Antibody inhibiting enzymatic activity of tumour-associated carbonic anhydrase isoform IX. Eur J Pharmacol. 2011 Feb 9. [PMID: 21315712]
  10. Kim M-Y, Jung J, Mo J-S, et al. The intracellular domain of Jagged-1 interacts with Notch1 intracellular domain and promotes its degradation through Fbw7 E3 ligase. Exp Cell Res. 2011 Jul 29. [PMID: 21820430]
  11. Silvestri, L, et al. Furin-mediated release of soluble hemojuvelin: a new link between hypoxia and iron homeostasis. Blood;111(2):924-31. 2008 Jan 15. [PMID: 17938254]
  12. Bello-Fernandez C, Packham G, Cleveland JL. The ornithine decarboxylase gene is a transcriptional target of c-Myc. Proc Natl Acad Sci U S A;90(16):7804-8. 1993 Aug 15. [PMID: 8356088]

Protocols

Product Specific (1)

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Jump to: Conjugated Antibodies, Positive Controls, Antibody Pairs, Lysates, Peptides and Proteins, Primary Antibodies, RNAi, Secondary Antibodies
  Conjugated Antibodies (5)
Name Catalog# Reactivity Applications Images Publications Reviews
c-Myc Antibody (9E10) [DyLight 650] NB600-302C Hu, Mu, Dr WB, ELISA, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP 1 0 0

c-Myc Antibody (9E10) [DyLight 550] NB600-302R Hu, Mu, Dr WB, ELISA, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP 1 0 0

c-Myc Antibody (9E10) [DyLight 488] NB600-302G Hu, Mu, Dr WB, ELISA, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP 1 0 0

c-Myc Antibody (9E10) [Biotin] NB600-302B Hu, Mu, Dr WB, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P 1 0 0

c-Myc Antibody (9E10) [HRP] NB600-302H Hu, Mu, Dr WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P, IP 1 0 0

  Positive Controls (2)
Name Catalog# Reactivity Applications Images Publications Reviews
Jurkat Whole Cell Lysate NB800-PC2 2 1 0

c-Myc Overexpression Lysate NBL1-13414 WB 1 0 0

  Antibody Pairs (1)
Name Catalog# Reactivity Applications Images Publications Reviews
c-Myc Antibody Pair H00004609-PW1 Hu WB, IP 0 0 0

  Lysates (1)
Name Catalog# Reactivity Applications Images Publications Reviews
c-Myc Overexpression Lysate NBL1-13414 WB 1 0 0

  Peptides and Proteins (3)
Name Catalog# Reactivity Applications Images Publications Reviews
c-Myc Recombinant Protein H00004609-P01 Hu WB, ELISA, PA 1 0 0

c-Myc Peptide NB600-336PEP NA 0 0 0

c-Myc Partial Recombinant Protein H00004609-Q01 Hu WB, ELISA, PA 1 0 0

  Primary Antibodies (65)
Name Catalog# Reactivity Applications Images Publications Reviews
c-Myc Antibody (9E11) NB200-108 Hu, Mu, Ch, Ye WB, ChIP, ELISA, FLOW, IHC, IHC-Fr, IHC-P, IP 1 9 3

c-Myc Antibody NB600-336 Hu, Mu WB, ChIP, ELISA, ICC/IF, IHC, IHC-P, IP 3 9 0

c-Myc Antibody NB600-335 NA WB, ELISA, ICC/IF 1 10 0

c-Myc Antibody NB600-338 NA WB, ELISA, ICC/IF 1 2 0

c-Myc Antibody (3F2) NBP1-47861 Hu WB, ICC/IF, IHC, IHC-P 14 0 0

... and 60 more. See All »
  RNAi (1)
Name Catalog# Reactivity Applications Images Publications Reviews
c-Myc RNAi H00004609-R01 Hu 1 0 0

  Secondary Antibodies (617)
Name Catalog# Reactivity Applications Images Publications Reviews
Rabbit anti-Mouse IgG Antibody [Alkaline Phosphatase] NB720-AP Mu WB, ELISA, ICC/IF, IHC, IHC-P 0 1 0

Rabbit anti-Mouse IgG Antibody [Biotin] NB720-B Mu WB, ELISA, IHC, IHC-P 0 0 0

Rabbit anti-Mouse IgG Antibody [HRP] NB720-H Mu WB, ELISA, ICC/IF, IHC, IHC-P 1 1 1

Goat anti-Mouse IgG Antibody [FITC] NB720-F Mu ICC/IF, IHC, IHC-P 0 3 0

Goat anti-Mouse IgG Antibody [HRP] NB7570 Mu WB, ELISA, ICC/IF, IHC, IHC-P 0 1 0

See All »
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c-Myc in Context

 
Research Areas related to c-Myc (10):
 
Novus Explorer for c-Myc gene