c-Myc Antibody (9E10) 0.1 ml

c-Myc NB600-302

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  • c-Myc Primary Antibodies
  • All c-Myc Products
  • 3 reviews  
  • 18 Publications
  • Alternate Names
     

Ordering Information: NB600-302

Catalog NumberNB600-302
  • FormUnconjugated
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c-Myc Antibody (9E10) Summary

 
Species Human, Mouse, Drosophila
Tested Applications WB, ChIP, ELISA, FLOW, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality Monoclonal
Host Mouse
Gene MYC
Purity Protein G purified
Specificity Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
Guarantee Plus All of our products are backed by our 100% guarantee to work for stated and predicted species and applications.
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c-Myc Antibody (9E10) Details

 
Clone 9E10
Isotype IgG1 Kappa
Epitope Leucine zipper sequence (AEEQKLISEEDLL)
Immunogen A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc. [UniProt# P01106]

Species Reactivity

 
Human, mouse and Drosophila.
Reviewed Species Read 3 rated 4.7  using NB600-302 in the following species:
 
Publications Read Publications using NB600-302 in the following species:
 

Applications/Dilutions

 
Dilutions
  • Western Blot 0.5-2.0 ug/ml
  • Chromatin Immunoprecipitation
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
Application Notes

This c-Myc antibody clone 9E10 is useful for Flow Cytometry (PMID: 21315712), Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Immunoprecipitation.

Positive Controls

2 Positive Controls

Other Available
Formats
7C Labeled NB600-302V2
Alexa Fluor (R) 405 Labeled NB600-302AF405
Alexa Fluor (R) 488 Labeled NB600-302AF488
Alexa Fluor (R) 647 Labeled NB600-302AF647
Alexa Fluor (R) 700 Labeled NB600-302AF700
Allophycocyanin Labeled NB600-302APC
Biotin Labeled NB600-302B
DyLight 350 Labeled NB600-302UV
DyLight 405 Labeled NB600-302V
DyLight 405LS Labeled NB600-302V3
DyLight 488 Labeled NB600-302G
DyLight 550 Labeled NB600-302R
DyLight 650 Labeled NB600-302C
DyLight 680 Labeled NB600-302FR
DyLight 755 Labeled NB600-302IR
FITC Labeled NB600-302F
HRP Labeled NB600-302H
PE Labeled NB600-302PE
PerCP Labeled NB600-302PCP
Learn about Custom Antibody Labeling
Reviewed Applications
Read 3 reviews rated 4.7  using
NB600-302 in the following applications:
Publications
Read publications using
NB600-302 in the following applications:

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Publications

c-Myc Antibody (NB600-302) has been mentioned in at least 18 publications.
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Protocols and FAQs

Controls & Support Products

Research Tools

View all c-Myc Research Tools.

Packaging, Storage & Formulations

 
Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer Tris-glycine, 150 mM NaCl
Unit Size 0.1 ml (also available in 0.025 ml (sample size))
Concentration 1 mg/ml
Preservative 0.05% Sodium Azide
Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

 
Gene Symbol MYC
Entrez
Uniprot

Alternate Names for c-Myc

 
  • BHLHE39
  • Proto-oncogene c-Myc
  • myc-related translation/localization regulatory factor
  • Class E basic helix-loop-helix protein 39
  • myc proto-oncogene protein
  • v-myc avian myelocytomatosis viral oncogene homolog
  • avian myelocytomatosis viral oncogene homolog
  • c-Myc
  • bHLHe39MRTL
  • Transcription factor p64
  • v-myc myelocytomatosis viral oncogene homolog (avian)

Related Products by Gene

Background

 
Myc genes are a family of proto-oncogenes (L- Myc, N- Myc and C- Myc) that codes for Myc proteins which are transcriptor factors implicated in cellular proliferation, differentiation, apoptosis, metabolism, adhesion and self-renovation of tumor stem cells. Myc protein can act as transcriptional activator/repressor, and is activated via response to diverse mitogenic signals (including Wnt, Shh and EGF) and has been found to be up-regulated in several types of cancers. c-Myc participates gene transcription regulation and binds DNA in a non-specific manner, yet can specifically recognizes core sequence 5'-CAC[GA]TG-3' also. c-Myc heterodimerization with another bHLH protein namely Myc-associated factor X (MAX) is required for efficient c-Myc- DNA binding. c-Myc interacts with several proteins such as TAF1C, SPAG9, PARP10, KDM5A, KDM5B, NO66, PIM2 and with FBXW7 when phosphorylated at Thr-58/Ser-62. c-Myc activate the transcription of growth-related genes and c- Myc overexpression induce cell-cycle progression thereby implicating in a variety of cancers. Moreover, a chromosomal aberration involving c-Myc has been linked to a form of B-cell chronic lymphocytic leukemia and defective c-MYC is responsible for Burkitt lymphoma also.