c-Myc Antibody (9E10) 0.1 ml

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Western Blot: c-Myc Antibody (9E10) [NB600-302] - Analysis of c-myc in Jurkat cell lysates using NB600-302.
Immunocytochemistry/Immunofluorescence: c-Myc Antibody (9E10) [NB600-302] - HeLa cells stained NB600-302 (Green) detected with DyLight Fluor 488 conjugated anti-mouse IgG secondary antibody.  Nuclei are counterstained with Hoechst 33258 (Blue).
Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate.  This experiment as performed under reducing conditions using the 12-230 kDa separation system.
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Ordering Information: NB600-302

  • Catalog Number
    NB600-302
  • Displayed Format
    Unconjugated
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c-Myc Antibody (9E10) Summary

Species Human, Mouse, Drosophila
Tested Applications WB, Simple Western, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
Clonality
Monoclonal
Host
Mouse
Gene
MYC
Purity
Protein G purified
Specificity
Specific for the c-myc protein in random coil configuration, not as a helix. 9E10 does not react with V-myc.
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c-Myc Antibody (9E10) Details

Clone
9E10
Isotype
IgG1 Kappa
Epitope
Leucine zipper sequence (AEEQKLISEEDLL)
Immunogen
A synthetic peptide corresponding to amino acids 408-439 (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) of human c-Myc. [UniProt# P01106]

Species Reactivity

Human, mouse and Drosophila.
Reviewed Species Read 3 rated 4.7
using NB600-302 in the following species:

Publications Read Publications using NB600-302 in the following species:


Applications/Dilutions

Dilutions
  • Western Blot 0.5-2.0 ug/ml
  • Simple Western 1:200
  • Chromatin Immunoprecipitation
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:50-1:200
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Frozen 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1:10-1:500
Application Notes
This c-Myc antibody clone 9E10 is useful for Flow Cytometry (PMID: 21315712), Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Immunoprecipitation.In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Positive Controls
Other Available
Formats
7C Labeled NB600-302V2
Alexa Fluor (R) 405 Labeled NB600-302AF405
Alexa Fluor (R) 488 Labeled NB600-302AF488
Alexa Fluor (R) 647 Labeled NB600-302AF647
Alexa Fluor (R) 700 Labeled NB600-302AF700
Allophycocyanin Labeled NB600-302APC
Biotin Labeled NB600-302B
DyLight 350 Labeled NB600-302UV
DyLight 405 Labeled NB600-302V
DyLight 405LS Labeled NB600-302V3
DyLight 488 Labeled NB600-302G
DyLight 550 Labeled NB600-302R
DyLight 650 Labeled NB600-302C
DyLight 680 Labeled NB600-302FR
DyLight 755 Labeled NB600-302IR
FITC Labeled NB600-302F
HRP Labeled NB600-302H
PE Labeled NB600-302PE
PerCP Labeled NB600-302PCP
Reviewed Applications
Read 3 Review rated 4.7
using
NB600-302 in the following applications:

Publications
Read Publications using
NB600-302 in the following applications:

Contact Information

Product PDFs

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Reviews

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Publications

c-Myc Antibody (NB600-302) has been mentioned in at least 19 publications.
Earn rewards if you have published using c-Myc Antibody (NB600-302).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-glycine, 150 mM NaCl
Unit Size
0.1 ml
Concentration
1 mg/ml
Preservative
0.05% Sodium Azide
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Gene Symbol MYC
Entrez
Uniprot

Alternate Names for c-Myc Antibody (9E10)

  • avian myelocytomatosis viral oncogene homolog
  • BHLHE39
  • bHLHe39MRTL
  • Class E basic helix-loop-helix protein 39
  • c-Myc
  • myc proto-oncogene protein
  • myc-related translation/localization regulatory factor
  • Proto-oncogene c-Myc
  • Transcription factor p64
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)
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Related Products by Gene

Background

Myc genes are a family of proto-oncogenes (L- Myc, N- Myc and C- Myc) that codes for Myc proteins which are transcriptor factors implicated in cellular proliferation, differentiation, apoptosis, metabolism, adhesion and self-renovation of tumor stem cells. Myc protein can act as transcriptional activator/repressor, and is activated via response to diverse mitogenic signals (including Wnt, Shh and EGF) and has been found to be up-regulated in several types of cancers. c-Myc participates gene transcription regulation and binds DNA in a non-specific manner, yet can specifically recognizes core sequence 5'-CAC[GA]TG-3' also. c-Myc heterodimerization with another bHLH protein namely Myc-associated factor X (MAX) is required for efficient c-Myc- DNA binding. c-Myc interacts with several proteins such as TAF1C, SPAG9, PARP10, KDM5A, KDM5B, NO66, PIM2 and with FBXW7 when phosphorylated at Thr-58/Ser-62. c-Myc activate the transcription of growth-related genes and c- Myc overexpression induce cell-cycle progression thereby implicating in a variety of cancers. Moreover, a chromosomal aberration involving c-Myc has been linked to a form of B-cell chronic lymphocytic leukemia and defective c-MYC is responsible for Burkitt lymphoma also.

Reviews for c-Myc Antibody (NB600-302) (3) 4.73

Read what people are saying who have used c-Myc Antibody (NB600-302).

We have 3 reviews tested in 1 species: Human.
We have 3 review tested in 1: Western Blot.
Average Rating: 4.7
(Based on 3 reviews)

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Sort 3 reviews for c-Myc Antibody (NB600-302) by:
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Excellent
(2)
Very Good
(1)
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WB
(3)
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Human
(3)
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Images Ratings Applications Species Reviewed By Date Details
  Excellent
WB Human 11/30/2011
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Summary

ApplicationWestern Blot
Sample TestedJurkat T-Cells
Sample PretreatmentRZPA Lysis
SpeciesHuman
CommentsGood!

Blocking

Blocking DetailsBlocking Buffer: 5% non-fat milk

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight

Details

Detection NotesDetection Method: ECL
  Very Good
WB Human 11/23/2010
Show

Summary

ApplicationWestern Blot
Sample TestedHeLa cell lysate, Sample Amount: 10 ul
Sample PretreatmentRIPA buffer
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: 20 degrees Celsius

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:500, Incubation Dilution Buffer: 5% Milk in PBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary Manufacturer Cat#NA9320

Details

Detection NotesDetection Method: ECL Plus, Exposure Time: 1 minute, Specific Bands: 53 kDa
  Excellent
WB Human 08/10/2010
Show

Summary

ApplicationWestern Blot
Sample Tested293T Cell Lysates overexpressing myc-tagged fusion proteins, Sample Amount: 50 ug
SpeciesHuman

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in PBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Publications for c-Myc Antibody (NB600-302) (19)

Read what people are writing who have used c-Myc Antibody (NB600-302).

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: WB, ELISA, Flow, ICC/IF, IP.

Have you published using c-Myc Antibody (NB600-302)? Submit your review and earn rewards points which can be used for merchandise & discounts.
Filter By Application
WB
(7)
ELISA
(1)
Flow
(1)
ICC/IF
(4)
IP
(6)
All Applications
Filter By Species
Human
(6)
Mouse
(2)
All Species
Publication Applications Species
Bello-Fernandez C, Packham G, ClevelJL. The ornithine decarboxylase gene is a transcriptional target of c-Myc. Proc Natl Acad Sci U S A;90(16):7804-8. 1993 Aug 15. [PMID: 8356088]
Silvestri, L et al. Furin-mediated release of soluble hemojuvelin: a new link between hypoxia and iron homeostasis. Blood;111(2):924-31. 2008 Jan 15. [PMID: 17938254]
Kim M-Y, Jung J, Mo J-S et al. The intracellular domain of Jagged-1 interacts with Notch1 intracellular domain and promotes its degradation through Fbw7 E3 ligase. Exp Cell Res. 2011 Jul 29. [PMID: 21820430]
Murri-Plesko MT, Hulikova A, Oosterwijk E et al. Antibody inhibiting enzymatic activity of tumour-associated carbonic anhydrase isoform IX. Eur J Pharmacol. 2011 Feb 9. [PMID: 21315712] (FLOW, ELISA) ELISA, Flow
Kari B et al. Characterization of monoclonal antibodies reactive to several biochemically distinct human cytomegalovirus glycoprotein complexes. J Virol;60(2):345-52. 1986 Nov. [PMID: 3021969] (IP, Human) IP Human
Hilpert K, Hansen G, Wessner H, Kuttner G, Welfle K, Seifert M, Hohne W. Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. Protein Eng;14(10):803-6. 2001 Oct. [PMID: 11739900]
Bergman, M et al. Cardiac matrix metalloproteinase-2 expression independently induces marked ventricular remodeling and systolic dysfunction. Am J Physiol Heart Circ Physiol;292(4):H1847-60. 2007 Apr. [PMID: 17158653] (WB, Mouse) WB Mouse
Trinkle-Mulcahy L, Chusainow J, Lam YW, Swift S, Lamond A. Visualization of intracellular PP1 targeting through transiently and stably expressed fluorescent protein fusions. Methods Mol Biol;365:133-54. 2007 [PMID: 17200560] (WB, IP, ICC/IF, Human) ICC/IF, IP, WB Human
Zhao Y, Wang Y, Hu J, Zhang X, Zhang YW. CutA divalent cation tolerance homolog (E. coli) (CUTA) regulates Beta-cleavage of Beta-amyloid precursor protein (APP) through interacting with Beta-Site APP cleaving protein 1 (BACE1) J Biol Chem 2012 Feb 17 [PMID: 22351782] (WB, Human) WB Human
Wang H, Shao Z, Shi LZ et al. CtIP Protein Dimerization Is Critical for Its Recruitment to Chromosomal DNA Double-stranded Breaks J Biol Chem 2012 Jun 15 [PMID: 22544744] (IP, WB, Human) IP, WB Human
Hubbi ME, Luo W, Baek JH et al. MCM proteins are negative regulators of hypoxia-inducible factor 1. Mol Cell. Jun. 2011 [PMID: 21658608] (IP, WB, Human) IP, WB Human
Santini E, Feyder M, Gangarossa G et al. Dopamine- and cAMP-regulated Phosphoprotein of 32-kDa (DARPP-32)-dependent Activation of Extracellular Signal-regulated Kinase (ERK) and Mammalian Target of Rapamycin Complex 1 (mTORC1) Signaling in Experimental Parkinsonism J Biol Chem 2012 Aug 10 [PMID: 22753408] (IP, Mouse) IP Mouse
Lu CS, Truong LN, Aslanian A et al. The RING finger protein RNF8 ubiquitinates Nbs1 to promote DNA double-strand break repair by homologous recombination J Biol Chem 2012 Oct 31 [PMID: 23115235] (IP) IP
Wang H, Shi LZ, Wong CC et al. The Interaction of CtIP and Nbs1 Connects CDK and ATM to Regulate HR-Mediated Double-Strand Break Repair. PLoS Genet 2013 Feb [PMID: 23468639] (WB) WB
Megill A, Lee T, Dibattista AM et al. A Tetra(Ethylene Glycol) Derivative of Benzothiazole Aniline Enhances Ras-Mediated Spinogenesis. J Neurosci 2013 May 29 [PMID: 23719799] (ICC/IF) ICC/IF
Hubbi ME, Hu H, Kshitiz NF et al. Sirtuin-7 Inhibits the Activity of Hypoxia-Inducible Factors J Biol Chem 2013 June 9 [PMID: 23750001]
Barnett TC, Liebl D, Seymour LM et al. The Globally Disseminated M1T1 Clone of Group A Streptococcus Evades Autophagy for Intracellular Replication. Cell Host Microbe 2013 Dec 11 [PMID: 24331465] (ICC/IF) ICC/IF
Pekkurnaz G, Trinidad JC, Wang X et al. Glucose Regulates Mitochondrial Motility via Milton Modification by O-GlcNAc Transferase. Cell 2014 Jul 03 [PMID: 24995978] (ICC/IF)

Details:
COS7 cells transfected with empty vector or myc-hMilton1 (Figure S3).
ICC/IF
Iampietro M, Morissette G, Gravel A, Flamand L. Inhibition of Interleukin-2 Gene Expression by Human Herpesvirus 6B U54 Tegument Protein. J Virol. 2014 Aug 13 [PMID: 25122797] (WB, Human) WB Human

Product General Protocols

View specific protocols for c-Myc Antibody (NB600-302): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

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FAQs for c-Myc (2)

  1. In the immunohistochemistry paraffin section protocol....the PBS buffer....should it be at certain pH?
    • For IHC application, different labs cites the pH of PBS buffer in the range of 7.1 - 7.6 but most commonly used pH is pH 7.4 (this is what we use in our lab). You may use this PBS (pH 7.4) for making permeablization buffer, antibody diluent buffers and for making wash buffer. For more on the protocol that we use in our lab and for IHC-P troubleshooting suggestions, you may visit: IHC-P Protocol and IHC-P Troubleshooting.
  2. I was wondering what does Format 7C mean as far as anitbodies go?
    • 7C is a fluorophore - it is useful for FLOW. The details of the fluor are (A=425, E=500)

Bioinformatics Tool for c-Myc

Discover related pathways, diseases and genes to c-Myc. Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Bioinformatics Tool

Blogs on c-Myc

Check out the latest blog posts on c-Myc.
c-Myc. See Myc Run Transcription Regulation
Myc genes (L-Myc, N-Myc and C-Myc) are a family of transcription factors. c-Myc is involved in transcription regulation, apoptosis and cell growth. Mutations in c-Myc have been tied to several cancers. Free sample bonus: Get a free sample...  Read full blog post.

Beta Catenin Implications for Signaling
The Wnt/beta Catenin signaling pathway plays a critical role in embryonic development, stem cell self-renewal and regeneration. Alterations in this signaling cascade have been implicated in the pathogenesis of cancer. Notably, chronic activation of Wn...  Read full blog post.

Cerebellar Degeneration-Related Protein 2 (CDR2): Cell-Cycle Regulated Tumor Antigen
CDR2 is a tumor antigen expressed in a high percentage of breast and ovarian tumors and is the target of a naturally occurring tumor immune response in patients with paraneoplastic cerebellar degeneration. CDR2 has also been shown to be a cell cycle r...  Read full blog post.

CIP2A: The Cancerous Inhibitor of Protein Phosphatase 2A
The autoantigen p90 is a recently discovered protein that binds to and inhibits Protein Phosphatase 2A (PP2A) activity, thereby playing a critical role in cancer progression. Thus, p90 was renamed the "cancerous inhibitory protein of PP2A" or CIP2...  Read full blog post.

Nucleolin Antibodies: Knowing When it's Time to Split
Nucleolin is an abundant, 106 kDa nucleolar phosphoprotein that is a major protein in actively dividing cells. The stability of nucleolin is heavily cell proliferation-dependent, as nucleolin antibody studies have shown that degraded forms are relativ...  Read full blog post.

The Myc Epitope Tags Along...
Recombinant DNA technology allows researchers to fuse epitope tags to their protein of interest and then identify that protein using tag specific antibodies. The Myc Tag can be used to purify tagged proteins by affinity chromatography or detect them b...  Read full blog post.

The c-Myc Antibody: A Major Tool in Cancer Research
C-Myc is a widely expressed transcription factor, regulating cellular differentiation, proliferation, cell cycle progression and pro-apoptotic gene expression. The c-Myc antibody is widely used in cancer research, as a number of human tumors have been...  Read full blog post.

Epitope Tags and the V5 Tag Antibody
We at Novus Biologicals have a large antibody catalog targeted to epitope tags. They include C-Myc, GFP, HA, DYKDDDDK, and V5 Tag antibody reagents. The V5 tag antibody recognizes the artificially engineered V5 epitope, which is formed of amino acid r...  Read full blog post.

Use Of c-Myc Antibodies In Non-Invasive Cancer Studies
Novus Biologicals specializes in offering top quality antibody reagents to many of the transcription factor proteins that are widely expressed in cancer cells.C-Myc is one of several transcription factor proteins covered by our antibody catalog. E...  Read full blog post.