Immunohistochemistry (IHC) is a common in vitro staining technique that uses antibodies to identify specific protein antigens within thinly sliced tissue samples. One of the most common forms of IHC uses tissues that are fixed with paraformaldehyde and then embedded in paraffin (IHC-P). However, because this preparation can mask the epitope that the antibody needs to detect, it is often recommended to perform antigen retrieval methods on paraffin embedded tissues. Specific antibodies are applied to the fixed tissue and typically visualized using either immunofluorescent (IF) or chromogenic reporters.
Novus offers a range of support options for IHC-P when you buy a product to help you with your IHC-P experiment including: