Immunocytochemistry (ICC) is a common in vitro staining technique that uses antibodies to identify the presence and localization of specific protein antigens within cultured cells. The antibodies are typically visualized using either immunofluorescence (IF) or chromogenic reporters. ICC experiments can be carried out in static fixed cells, or in real time by using live cells. By using immunofluorescent reporters, researchers are able to multiplex antibodies within the sample by utilizing reporters of varying wavelengths.
Novus offers a range of support options for ICC/IF when you buy a product to help you with your ICC/IF experiment including: