Chromatin Immunoprecipitation (ChIP) is a research assay used to explore the direct interaction between DNA elements and various proteins. Specifically, this method can be used to study transcription factors, DNA binding elements and other proteins that associate with specific DNA domains. The process temporarily links protein to its associated DNA, which are then selectively immunoprecipitated using antibodies specific to the protein of interest. The DNA fragments are then unbound and analyzed using a variety of methods such as end point or real time PCR, sequencing, or microarrays. This technique has proven to be extremely useful for researchers studying in vivo interactions between particular proteins and unknown DNA regions.
Novus offers a range of support options for ChIP when you buy a product to help you with your ChIP experiment including: