The Survivin antibody is widely used in apoptosis studies, and as a cellular marker. Today, biochemists have a wide range of Survivin antibody products to choose from, suitable for use in Western Blot, Chromatin Immunoprecipitation (ChIP), Immunofluorescence and similar assays.
ELISA (Enzyme-linked immunosorbent assay) is a popular tool in Survivin antibody research. In simple terms, ELISA involves binding of an antigen to an enzyme-linked antibody, which must be highly specific. Various formats of this basic technique have been developed, the most powerful of which is the Sandwich ELISA assay. Sandwich ELISA is dependent upon antigens with at least two binding sites, as it uses both a capture and a detection antibody, between which the sample is “sandwiched.” This has several benefits over standard ELISA, including increased sensitivity and the fact samples do not need to be purified prior to analysis.
The one disadvantage with sandwich ELISA is that antibody optimization can be difficult. It’s essential each antibody reacts with a specific epitope on the target protein and does not cross-react with its partner, and so only products specifically tested for sandwich ELISA should be used. We at Novus Biologicals have achieved this with our sandwich ELISA kits, such as our human Survivin antibody ELISA kit.
The capture antibody of this quality assured kit is polyclonal human Survivin antibody, coated onto the 96-well strip plates, which are provided. Having added human sera or test standards to these plates, a biotinylated detection antibody is added, completing the sandwich. Survivin detection is achieved via an ABC-complex enzyme, which binds to the detection antibody. A peroxidase substrate induces a quantitative coloured reaction. This kit is ideal when Survivin antibody testing of human tissue samples is required.