AKT Products

AKT Antibodies

AF887
Flow Cytometry: AKT [p Ser473] Pan Specific Antibody [AF887] - Akt phosphorylated at S473 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human/Mouse/Rat Phospho-Akt (S473) Antigen Affinity-purified Polyclonal Antibody (R&D Systems catalog # AF887) at 10 ug/mL overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; R&D Systems catalog # CTS005) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.Flow Cytometry: AKT [p Ser473] Pan Specific Antibody [AF887] - Lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (R&D Systems catalog # 120-HD) for 15 minutes and MCF-7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (R&D Systems catalog # 291-G1) for 15 minutes. PVDF membrane was probed with 0.5 ug/mL of Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (R&D Systems catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (R&D Systems catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions.
Species: Human, Mouse, Rat
Applications: WB, Simple Western, IHC, Flow
Host: Rabbit Polyclonal
MAB2055
Western Blot: AKT Antibody (281046) [MAB2055] - Lysates of MCF-7 human breast cancer cell line, MBA-MB-123 human breast cancer cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.2 ug/mL Mouse Anti-Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody (R&D Systems catalog # MAB2055) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (R&D Systems catalog # HAF007). This experiment was conducted under reducing conditions.Immunocytochemistry/Immunofluorescence: AKT Antibody (281046) [MAB2055] - Akt was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human/ Mouse/Rat Akt Monoclonal Antibody (R&D Systems catalog # MAB2055) at 10 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; R&D Systems catalog # NL007) and counterstained with DAPI (blue).
Species: Human, Mouse, Rat
Applications: WB, Simple Western, ICC, Flow
Host: Mouse Monoclonal
MAB887
Western Blot: AKT Antibody (545007) [MAB887] - Lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 nm Calyculin for 30 minutes, 1 ug/mL insulin for 5 minutes, or 100 ng/mL Recombinant Human PDGF-AB (R&D Systems catalog # 222-AB) for 20 minutes. PVDF membrane was probed with 0.2 ug/mL of Mouse Anti-Human Phospho-Akt (S473) Pan Specific Monoclonal Antibody (R&D Systems catalog # MAB887) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (R&D Systems catalog # HAF007). A specific band was detected for Phospho-Akt (S473) at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions.Simple Western: AKT [p Ser473] Antibody (545007) [MAB887] - Simple Western lane view shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 65 kDa (as indicated) using 2 ug/mL of Mouse Anti-Human/Mouse Phospho-Akt (S473) Pan Specific Monoclonal Antibody (Catalog # MAB887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Species: Human, Mouse
Applications: WB, Simple Western
Host: Mouse Monoclonal

Bioinformatics

Product By Gene ID 207

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