Stem Cells eNews - 1st Edition

Spotlight on Core-ESC Like Genes
By comparing mouse and human ESC-like gene modules, a recent study has defined a panel of 335 genes as the "core ESC-like gene module" (Wong, D. J. et al. Module map of stem cell genes guides creation of epithelial cancer stem cells. Cell Stem Cell (2008). 10;2(4):333-44. [PMID: 18397753]). These genes were often induced in many aggressive human epithelial cancers. Tumors with the ESC-activated signature were significantly associated with poorer tumor differentiation, higher mortality rates and more frequently progressed to metastasis.

Check out these Novus antibodies and reagents for Core ESC-Like Genes.

Coming Soon: Customer Reviews Published on our Website
Survivin Antibody [NB500-201]

This antibody was given five stars in a customer review submitted on July 2nd, 2009.  The customer conducted a Western Blot analysis on cancer cell lines at a 1:1,000 dilution. The survivin antibody was incubated for 2 hours at 20 degrees Celsius in 1X TBS-Tween.  The secondary antibody used was goat anti-rabbit, conjugated to peroxidase at a dilution of 1:2,000.  The customer loaded 7-15ug of lysate on a 4-12% Bis-Tris gel.

  Customer review by Jenette Shoeneman jenkat@rams.colostate.edu

New Product Information
• 25% OFF Select Stem Cell Antibodies and Reagents. View the list here.

• DGCR8 Knockout Mouse Embryonic Stem Cells [NBA1-19349]
  Novus Biologicals now offers a novel a line of DGCR8 knockout mouse ESCs to study   the global role of microRNAs. ESCs provide a tool for the study of the molecular   mechanisms of early mammalian development. The DGCR8 cells have been   genetically altered at the locus of the dgcr8 gene such that no functional DGCR8   protein can be produced, resulting in the global, but specific, loss of micro   RNAs.Read more.

Publications
1. [NB100-74356 alpha Catenin] Bajpai S, Feng Y, Krishnamurthy R, et al. Loss of alpha-catenin decreases the strength of single E-cadherin bonds between human cancer cells. J Biol Chem 2009;284(27):18252-18259. [PMID: 19458087]

2. [NB100-101 APE1] Lam W, Bussom S, Cheng Y-C. Effect of hypoxia on the expression of phosphoglycerate kinase and antitumor activity of troxacitabine and gemcitabine in non-small cell lung carcinoma. Mol Cancer Ther. 2009;8(2):415-423. [PMID: 19208827]

3. [NB100-116 APE1] Batuello CN, Kelley MR, Dynlacht JR. Role of Ape1 and base excision repair in the radioresponse and heat-radiosensitization of HeLa Cells. Anticancer Res 2009;29(4):1319-1325 [PMID: 19414382]

4. [NB100-212 Aurora A] Gupta MK, Papay RS, Jurgens CWD, et al. Alpha 1-Adrenergic Receptors Regulate Neurogenesis and Gliogenesis. Mol Pharmacol 2009;76(2):314-326. [PMID: 19487244]